产品简要
公司名称 :
QED Bioscience
产品类型 :
抗体
产品名称 :
抗HIF1甲抗体
目录 :
34039
规格 :
100微克
价格 :
413美元
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
ESEE122
反应物种 :
人类, 小鼠
更多信息或购买 :
图像
图像 1 :
Immunohistochemistry analysis using Mouse Anti-HIF1 alpha Monoclonal Antibody, Clone ESEE122 (34039). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-HIF1 alpha Monoclonal Antibody (34039) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Membranous and cytoplasmic localization in the epidermis, positive hair follicles, muscle and dermis.
图像 2 :
Western Blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of HIF1 alpha protein using Mouse Anti-HIF1 alpha Monoclonal Antibody, Clone ESEE122 (34039). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-HIF1 alpha Monoclonal Antibody (34039) at 1:500 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
产品信息
目录号 :
34039
产品名称 :
抗HIF1甲抗体
NAME+SKU :
抗HIF1甲抗体(34039)
产品_TYPE :
抗体
描述 :
抗HIF1甲小鼠单克隆抗体
SPECIFICITY SHORT :
HIF1甲
物种活性 :
人类
靶标名 :
HIF1甲
UniProt数据库 :
Q61221
LOCATION :
Cytoplasm , Nucleus , Nucleus speckle
蛋白名 :
缺氧诱导因子甲
别称 :
HIF-1-alpha, HIF1-alpha, ARNT-interacting protein
基因名称 :
缺氧诱导因子1A
克隆性 :
单克隆
形式 :
液体
浓度 :
Lot Specific
规格1 :
100微克
标价规格1 :
413美元
DISTIRBUTOR价格 :
289.1
SKU号规格1 :
34039
应用 :
免疫印迹, 免疫组化, 免疫细胞化学
应用INSTRUCTIONS :
Immunoblotting: use at 2ug/ml. Predicted molecular weight ~116kDa.
This is a recommended concentration. Endusers should determine optimal concentrations for their applications.
宿主 :
小鼠
格式 :
纯化
纯化 :
经免疫亲和层析纯化
抗体亚型 :
IgG1
克隆编号 :
ESEE122
免疫原 :
Recombinant protein corresponding to aa 329-530 of human HIF-1alpha.
研究领域 :
Cancer research
配方 :
PBS, pH 7.4; 50% glycerol, 0.09% sodium azide.
Purified by Protein G affinity chromatography.
缓冲液 :
磷酸盐缓冲液
缓冲液pH值 :
pH值7.4
缓冲液抗MICROBIAL :
0.09% 叠氮化钠
缓冲液CRYOPRESERVATIVE :
50% 甘油
靶标背景 :
Hypoxia-inducible factor 1 (HIF-1) is a heterodimeric transcription factor that regulates the transcription of a broad range of genes that facilitate responses to hypoxia including genes that regulate angiogenesis, erythropoiesis, cell cycle, metabolism, and apoptosis. HIF-1 is comprised of two subunits, HIF-1alpha and HIF-1beta. The widely expressed HIF-1alpha is degraded rapidly in normoxic cells by the ubiquitin/proteasomal pathway. HIF-1alpha is proline hydroxylated leading to a conformational change that promotes binding to the von Hippel Lindau protein (VLH)-E3 ligase complex; ubiquitination and proteasomal degradation follows.
FUNCTION :
Functions as a master transcriptional regulator of the adaptive response to hypoxia (PubMed:15225651, PubMed:17981124, PubMed:22009797). Under hypoxic conditions, activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia (PubMed:15225651, PubMed:17981124, PubMed:22009797). Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease (PubMed:22009797). Heterodimerizes with ARNT; heterodimer binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters (PubMed:26245371). Activation requires recruitment of transcriptional coactivators such as CREBBP and EP300. Activity is enhanced by interaction with NCOA1 and/or NCOA2. Interaction with redox regulatory protein APEX1 seems to activate CTAD and potentiates activation by NCOA1 and CREBBP. Involved in the axonal distribution and transport of mitochondria in neurons during hypoxia (By similarity). {UniProtKB:Q16665, PubMed:15225651, PubMed:17981124, PubMed:22009797, PubMed:26245371}.
稀释INSTRUCTIONS :
Dilute in PBS or medium that is identical to that used in the assay system.
STORAGE空调 :
This antibody is stable for at least one (1) year at -20C. Avoid multiple freeze- thaw cycles.
特异性 :
这抗体识别人类, 小鼠, 大鼠, 和牛HIF-1alpha
图像Name_1 :
qed-bioscience-anti-hif1-alpha-antibody-34039-1.png
应用1 :
免疫组化
图像Name_2 :
qed-bioscience-anti-hif1-alpha-antibody-34039-2.png
应用2 :
免疫印迹
更多信息或购买 :
公司信息
QED Bioscience
10919 Technology Place, Suite C San Diego, CA 92127
info@qedbio.com
http://www.qedbio.com800-929-2114 858-675-2405
公司总部: 美国