抗体

抗GSK3乙(磷酸化Ser9)抗体

43002

100微升

345美元

多克隆

domestic rabbit

未共轭

人类, 小鼠, 大鼠

抗GSK3乙(磷酸化Ser9)抗体

抗GSK3乙(磷酸化Ser9)抗体(43002)

抗体

抗GSK3乙(磷酸化Ser9)兔多克隆抗体

GSK3乙(磷酸化Ser9)

人类, 小鼠, 大鼠

GSK3乙(磷酸化Ser9)

磷酸化ser9

P49841

Cytoplasm , Nucleus , Cell membrane

糖原合酶激酶3乙

GSK3B

多克隆

液体

1.0毫克/毫升

100微升

345美元

241.5

43002

免疫印迹, 免疫组化

Western blotting: use at dilution of 1:500- 1:1,000. A band of ~46kDa is detected. Immunohistochemistry: use at dilution of 1:50- 1:100. These are recommended working dilutions. Enduser should determine optimal dilutions for their applications.

兔

纯化

经免疫亲和层析纯化

Peptide sequence that includes phosphorylation site of serine 9 (T-T-S(p)-F-A) derived from Human GSK3beta and conjugated to KLH.

磷酸化特异性抗体

PBS (without Mg2 and Ca2 ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

磷酸盐缓冲液

pH值7.4

0.02% 叠氮化钠

50% 甘油

GSK3beta participates in the Wnt signaling pathway and is implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN. GSK3beta phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA. It also phosphorylates CTNNB1/beta-catenin and MUC1 in breast cancer cells and decreases the interaction of MUC1 with CTNNB1/beta-catenin.

Constitutively active protein kinase that acts as a negative regulator in the hormonal control of glucose homeostasis, Wnt signaling and regulation of transcription factors and microtubules, by phosphorylating and inactivating glycogen synthase (GYS1 or GYS2), EIF2B, CTNNB1/beta-catenin, APC, AXIN1, DPYSL2/CRMP2, JUN, NFATC1/NFATC, MAPT/TAU and MACF1 (PubMed:1846781, PubMed:9072970, PubMed:14690523, PubMed:20937854, PubMed:12554650, PubMed:11430833, PubMed:16484495). Requires primed phosphorylation of the majority of its substrates (PubMed:11430833, PubMed:16484495). In skeletal muscle, contributes to insulin regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity and hence glycogen synthesis (PubMed:8397507). May also mediate the development of insulin resistance by regulating activation of transcription factors (PubMed:8397507). Regulates protein synthesis by controlling the activity of initiation factor 2B (EIF2BE/EIF2B5) in the same manner as glycogen synthase (PubMed:8397507). In Wnt signaling, GSK3B forms a multimeric complex with APC, AXIN1 and CTNNB1/beta-catenin and phosphorylates the N-terminus of CTNNB1 leading to its degradation mediated by ubiquitin/proteasomes (PubMed:12554650). Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA (PubMed:1846781). Phosphorylates NFATC1/NFATC on conserved serine residues promoting NFATC1/NFATC nuclear export, shutting off NFATC1/NFATC gene regulation, and thereby opposing the action of calcineurin (PubMed:9072970). Phosphorylates MAPT/TAU on 'Thr-548', decreasing significantly MAPT/TAU ability to bind and stabilize microtubules (PubMed:14690523). MAPT/TAU is the principal component of neurofibrillary tangles in Alzheimer disease (PubMed:14690523). Plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex (PubMed:20937854). Phosphorylates MACF1, inhibiting its binding to microtubules which is critical for its role in bulge stem cell migration and skin wound repair (By similarity). Probably regulates NF-kappa-B (NFKB1) at the transcriptional level and is required for the NF-kappa-B-mediated anti-apoptotic response to TNF-alpha (TNF/TNFA) (By similarity). Negatively regulates replication in pancreatic beta-cells, resulting in apoptosis, loss of beta-cells and diabetes (By similarity). Through phosphorylation of the anti-apoptotic protein MCL1, may control cell apoptosis in response to growth factors deprivation (By similarity). Phosphorylates MUC1 in breast cancer cells, decreasing the interaction of MUC1 with CTNNB1/beta-catenin (PubMed:9819408). Is necessary for the establishment of neuronal polarity and axon outgrowth (PubMed:20067585). Phosphorylates MARK2, leading to inhibit its activity (By similarity). Phosphorylates SIK1 at 'Thr-182', leading to sustain its activity (PubMed:18348280). Phosphorylates ZC3HAV1 which enhances its antiviral activity (PubMed:22514281). Phosphorylates SNAI1, leading to its BTRC-triggered ubiquitination and proteasomal degradation (PubMed:15448698, PubMed:15647282). Phosphorylates SFPQ at 'Thr-687' upon T-cell activation (PubMed:20932480). Phosphorylates NR1D1 st 'Ser-55' and 'Ser-59' and stabilizes it by protecting it from proteasomal degradation. Regulates the circadian clock via phosphorylation of the major clock components including ARNTL/BMAL1, CLOCK and PER2 (PubMed:19946213, PubMed:28903391). Phosphorylates CLOCK AT 'Ser-427' and targets it for proteasomal degradation (PubMed:19946213). Phosphorylates ARNTL/BMAL1 at 'Ser-17' and 'Ser-21' and primes it for ubiquitination and proteasomal degradation (PubMed:28903391). Phosphorylates OGT at 'Ser-3' or 'Ser-4' which positively regulates its activity. Phosphorylates MYCN in neuroblastoma cells which may promote its degradation (PubMed:24391509). Regulates the circadian rhythmicity of hippocampal long-term potentiation and ARNTL/BMLA1 and PER2 expression (By similarity). Acts as a regulator of autophagy by mediating phosphorylation of KAT5/TIP60 under starvation conditions, leading to activate KAT5/TIP60 acetyltransferase activity and promote acetylation of key autophagy regulators, such as ULK1 and RUBCNL/Pacer (PubMed:30704899). Negatively regulates extrinsic apoptotic signaling pathway via death domain receptors. Promotes the formation of an anti-apoptotic complex, made of DDX3X, BRIC2 and GSK3B, at death receptors, including TNFRSF10B. The anti-apoptotic function is most effective with weak apoptotic signals and can be overcome by stronger stimulation (PubMed:18846110). Phosphorylates E2F1, promoting the interaction between E2F1 and USP11, leading to stabilize E2F1 and promote its activity (PubMed:17050006, PubMed:28992046). {UniProtKB:P18266, UniProtKB:Q9WV60, PubMed:11430833, PubMed:12554650, PubMed:14690523, PubMed:15448698, PubMed:15647282, PubMed:16484495, PubMed:17050006, PubMed:18348280, PubMed:1846781, PubMed:18846110, PubMed:19946213, PubMed:20067585, PubMed:20932480, PubMed:20937854, PubMed:22514281, PubMed:24391509, PubMed:28903391, PubMed:28992046, PubMed:30704899, PubMed:8397507, PubMed:9072970, PubMed:9819408}.

Dilute in PBS or medium that is identical to that used in the assay system.

This antibody is stable for at least one (1) year at -20C. Can be stored at 4C for short-term.

This antibody detects endogenous human, mouse, and rat GSK3 beta only when phosphorylated at serine 9.