抗体

抗Raf1(磷酸化Ser259)抗体

43006

100微升

345美元

多克隆

domestic rabbit

未共轭

人类, 小鼠, 大鼠

抗Raf1(磷酸化Ser259)抗体

抗Raf1(磷酸化Ser259)抗体(43006)

抗体

抗Raf1(磷酸化Ser259)兔多克隆抗体

Raf1(磷酸化Ser259)

人类, 小鼠, 大鼠

Raf1(磷酸化Ser259)

磷酸化ser259

P04049

Cytoplasm. Cell membrane. Mitochondrion. Nucleus. Note=Colocalizes with RGS14 and BRAF in both the cytoplasm and membranes. Phosphorylation at Ser-259 impairs its membrane accumulation. Recruited to the cell membrane by the active Ras protein. Phosphorylation at Ser-338 and Ser-339 by PAK1 is required for its mitochondrial localization. Retinoic acid-induced Ser-621 phosphorylated form of RAF1 is predominantly localized at the nucleus.

RAF原癌基因丝氨酸/苏氨酸蛋白激酶

RAF1

多克隆

液体

1.0毫克/毫升

100微升

345美元

241.5

43006

IF, 免疫组化

Western blotting: use at dilution of 1:500- 1:1,000. A band of ~73kDa is detected. Immunohistochemistry: use at dilution of 1:50- 1:100. These are recommended working dilutions. Enduser should determine optimal dilutions for their applications.

兔

纯化

经免疫亲和层析纯化

Peptide sequence that includes phosphorylation site of serine 259 (S-T-S(p)-T-P) derived from human Raf1 and conjugated to KLH.

磷酸化特异性抗体

PBS (without Mg2 and Ca2 ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

磷酸盐缓冲液

pH值7.4

0.02% 叠氮化钠

50% 甘油

RAF proto-oncogene serine/threonine-protein kinase, also known as Raf1, is part of the ERK1/2 pathway as a MAP kinase kinase kinase (MAP3K) that functions downstream of the Ras subfamily of membrane associated GTPases. Raf1 is a member of the Raf kinase family of serine/threonine-specific protein kinases from the TKL (Tyrosine-kinase-like) group of kinases.

Serine/threonine-protein kinase that acts as a regulatory link between the membrane-associated Ras GTPases and the MAPK/ERK cascade, and this critical regulatory link functions as a switch determining cell fate decisions including proliferation, differentiation, apoptosis, survival and oncogenic transformation. RAF1 activation initiates a mitogen-activated protein kinase (MAPK) cascade that comprises a sequential phosphorylation of the dual-specific MAPK kinases (MAP2K1/MEK1 and MAP2K2/MEK2) and the extracellular signal-regulated kinases (MAPK3/ERK1 and MAPK1/ERK2). The phosphorylated form of RAF1 (on residues Ser-338 and Ser-339, by PAK1) phosphorylates BAD/Bcl2-antagonist of cell death at 'Ser-75'. Phosphorylates adenylyl cyclases: ADCY2, ADCY5 and ADCY6, resulting in their activation. Phosphorylates PPP1R12A resulting in inhibition of the phosphatase activity. Phosphorylates TNNT2/cardiac muscle troponin T. Can promote NF-kB activation and inhibit signal transducers involved in motility (ROCK2), apoptosis (MAP3K5/ASK1 and STK3/MST2), proliferation and angiogenesis (RB1). Can protect cells from apoptosis also by translocating to the mitochondria where it binds BCL2 and displaces BAD/Bcl2-antagonist of cell death. Regulates Rho signaling and migration, and is required for normal wound healing. Plays a role in the oncogenic transformation of epithelial cells via repression of the TJ protein, occludin (OCLN) by inducing the up-regulation of a transcriptional repressor SNAI2/SLUG, which induces down-regulation of OCLN. Restricts caspase activation in response to selected stimuli, notably Fas stimulation, pathogen-mediated macrophage apoptosis, and erythroid differentiation. {PubMed:11427728, PubMed:11719507, PubMed:15385642, PubMed:15618521, PubMed:15849194, PubMed:16892053, PubMed:16924233, PubMed:9360956}.

Dilute in PBS or medium that is identical to that used in the assay system.

This antibody is stable for 1 month at 4°C or one year at -20°C. Avoid multiple freeze-thaw cycles.

This antibody detects endogenous human, mouse, and rat Raf1 only when phosphorylated at serine 259.