产品简要
公司名称 :
QED Bioscience
产品类型 :
抗体
产品名称 :
抗磷酸酶张力蛋白同源基因(磷酸化Ser380)抗体
目录 :
43009
规格 :
100微升
价格 :
345美元
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
反应物种 :
人类, 小鼠, 大鼠
更多信息或购买 :
产品信息
目录号 :
43009
产品名称 :
抗磷酸酶张力蛋白同源基因(磷酸化Ser380)抗体
NAME+SKU :
抗磷酸酶张力蛋白同源基因(磷酸化Ser380)抗体(43009)
产品_TYPE :
抗体
描述 :
抗磷酸酶张力蛋白同源基因(磷酸化Ser380)兔多克隆抗体
SPECIFICITY SHORT :
磷酸酶张力蛋白同源基因(磷酸化Ser380)
物种活性 :
人类, 小鼠, 大鼠
靶标名 :
磷酸酶张力蛋白同源基因(磷酸化Ser380)
靶抗原修饰 :
磷酸化ser380
UniProt数据库 :
P60484
LOCATION :
Cytoplasm , Nucleus , Nucleus, PML body
蛋白名 :
Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN
基因名称 :
磷酸酶张力蛋白同源基因
克隆性 :
多克隆
形式 :
液体
浓度 :
1.0毫克/毫升
规格1 :
100微升
标价规格1 :
345美元
DISTIRBUTOR价格 :
241.5
SKU号规格1 :
43009
应用 :
IF, 免疫组化
应用INSTRUCTIONS :
Immunofluorescence: use at dilution of 1:100- 1:200. Immunohistochemistry: use at dilution of 1:50- 1:100. These are recommended working dilutions. Enduser should determine optimal dilutions for their application.
宿主 :
格式 :
纯化
纯化 :
经免疫亲和层析纯化
免疫原 :
Peptide sequence that includes phosphorylation site of serine 380 derived from human PTEN and conjugated to KLH.
研究领域 :
磷酸化特异性抗体
配方 :
PBS (without Mg2 and Ca2 ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
缓冲液 :
磷酸盐缓冲液
缓冲液pH值 :
pH值7.4
缓冲液抗MICROBIAL :
0.02% 叠氮化钠
缓冲液CRYOPRESERVATIVE :
50% 甘油
靶标背景 :
Phosphatase and tensin homolog (PTEN) was identified as a tumor suppressor that is mutated in many cancers. PTEN is a phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase. It contains a tensin-like domain as well as a catalytic domain similar to the dual specificity protein tyrosine phosphatases. Unlike most protein tyrosine phosphatases, PTEN preferentially dephosphorylates phosphoinositide substrates. It negatively regulates intracellular levels of phosphatidylinositol- 3,4,5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating Akt/PKB signaling pathway.
FUNCTION :
Tumor suppressor. Acts as a dual-specificity protein phosphatase, dephosphorylating tyrosine-, serine- and threonine-phosphorylated proteins. Also acts as a lipid phosphatase, removing the phosphate in the D3 position of the inositol ring from phosphatidylinositol 3,4,5-trisphosphate, phosphatidylinositol 3,4-diphosphate, phosphatidylinositol 3-phosphate and inositol 1,3,4,5-tetrakisphosphate with order of substrate preference in vitro PtdIns(3,4,5)P3 > PtdIns(3,4)P2 > PtdIns3P > Ins(1,3,4,5)P4 (PubMed:26504226, PubMed:16824732). The lipid phosphatase activity is critical for its tumor suppressor function. Antagonizes the PI3K-AKT/PKB signaling pathway by dephosphorylating phosphoinositides and thereby modulating cell cycle progression and cell survival. The unphosphorylated form cooperates with MAGI2 to suppress AKT1 activation. Dephosphorylates tyrosine-phosphorylated focal adhesion kinase and inhibits cell migration and integrin-mediated cell spreading and focal adhesion formation. Plays a role as a key modulator of the AKT-mTOR signaling pathway controlling the tempo of the process of newborn neurons integration during adult neurogenesis, including correct neuron positioning, dendritic development and synapse formation. May be a negative regulator of insulin signaling and glucose metabolism in adipose tissue. The nuclear monoubiquitinated form possesses greater apoptotic potential, whereas the cytoplasmic nonubiquitinated form induces less tumor suppressive ability. In motile cells, suppresses the formation of lateral pseudopods and thereby promotes cell polarization and directed movement. {PubMed:16824732, PubMed:26504226}.; [Isoform alpha]: Functional kinase, like isoform 1 it antagonizes the PI3K-AKT/PKB signaling pathway. Plays a role in mitochondrial energetic metabolism by promoting COX activity and ATP production, via collaboration with isoform 1 in increasing protein levels of PINK1.
稀释INSTRUCTIONS :
Dilute in PBS or medium that is identical to that used in the assay system.
STORAGE空调 :
This antibody is stable for at least one (1) year at -20C. Can be stored at 4C for short-term.
特异性 :
This antibody detects endogenous human, mouse, and rat PTEN only when phosphorylated at serine 380.
更多信息或购买 :
公司信息
QED Bioscience
10919 Technology Place, Suite C San Diego, CA 92127
info@qedbio.com
http://www.qedbio.com
800-929-2114 858-675-2405
公司总部: 美国