抗体

抗HER2(磷酸化Tyr1221/Try1222)抗体

43076

100微升

345美元

多克隆

domestic rabbit

未共轭

抗HER2(磷酸化Tyr1221/Try1222)抗体

抗HER2(磷酸化Tyr1221/Try1222)抗体(43076)

抗体

抗HER2(磷酸化Tyr1221/Try1222)兔多克隆抗体

HER2(磷酸化Tyr1221/Try1222)

人类

HER2(磷酸化Tyr1221/Try1222)

磷酸化tyr1221

P04626

[Isoform 1]: Cell membrane , Cytoplasm, perinuclear region. Nucleus. Note=Translocation to the nucleus requires endocytosis, probably endosomal sorting and is mediated by importin beta-1/KPNB1. Also detected in VPS35-positive endosome-to-TGN retrograde vesicles (PubMed:31138794).

Receptor tyrosine-protein kinase erbB-2

v-erb-b2成红细胞白血病病毒致瘤基因2

多克隆

液体

1.0毫克/毫升

100微升

345美元

241.5

43076

免疫印迹, IF

Immunoblotting: use at dilution of 1:500-1:1,000. A band of ~185kDa is detected. Immunofluorescence: use at dilution of 1:100-1:200. These are recommended working dilutions. Endusers should determine optimal dilutions for their applications.

兔

纯化

经免疫亲和层析纯化

Peptide sequence that includes phosphorylation site of tyrosine 1221/1222 (N-L-Y(p)- Y(p)-W) derived from human HER2 and conjugated to KLH.

磷酸化特异性抗体

PBS (without Mg2 and Ca2 ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

磷酸盐缓冲液

pH值7.4

0.02% 叠氮化钠

50% 甘油

HER2 is a member of the human epidermal growth factor receptor (HER/EGFR/ERBB) family. Amplification or over-expression of this oncogene has been shown to play an important role in the development and progression of certain aggressive types of breast cancer. In recent years HER2 has become an important biomarker and target of therapy for approximately 30% of breast cancer patients.

Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization. {PubMed:10358079, PubMed:15380516, PubMed:21555369}.

Dilute in PBS or medium that is identical to that used in the assay system.

This antibody is stable for at least one (1) year at -70C. Avoid multiple freeze- thaw cycles.

This antibody detects endogenous human HER2 only when phosphorylated at tyrosine 1221/1222.Accession no.: P04626, NP_001005862.1