抗体

抗CD74抗体

56451

100微克

371美元

单克隆

小鼠

未共轭

PIN1

抗CD74抗体

抗CD74抗体(56451)

抗体

抗CD74小鼠单克隆抗体

CD74

人类

CD74

P04233

Cell membrane , Endoplasmic reticulum membrane. Golgi apparatus, trans-Golgi network. Endosome. Lysosome. Note=Transits through a number of intracellular compartments in the endocytic pathway. It can either undergo proteolysis or reach the cell membrane.; [Isoform p41]: Late endosome , Lysosome

HLA组织相容性抗原II类丙链

HLA-DR antigens-associated invariant chain, Ia antigen-associated invariant chain, Ii, CD antigen CD74 [Cleaved into: Class-II-associated invariant chain peptide

CD74

单克隆

液体

Lot Specific

100微克

371美元

259.7

56451

免疫印迹, 免疫沉淀, 免疫细胞化学, 流式细胞仪

Immunoblotting: use at 1ug/ml. A doublet band of ~33-35 kDa is detected. Immunoprecipitation: use at 12ug/ml. Immunocytochemistry: working concentration is sample dependent. Flow cytometry: working concentration is sample dependent. These are recommended concentrations. User should determine optimal concentrations for their application. Positive control: PALA cell lysate.

小鼠

纯化

经免疫亲和层析纯化

IgG1

PIN1

Synthetic peptide corresponding to the human CD74 invariant chain (aa 12-28) conjugated to KLH.

神经科学

PBS, pH值7.4

磷酸盐缓冲液

pH值7.4

CD74 is a non-polymorphic type II integral membrane protein. It has a short N-terminal cytoplasmic tail of 28 amino acids followed by a single 24-aa transmembrane region and an approximately 150-aa lumenal domain. CD74 functions mainly as an MHC class II chaperone which promotes ER exit of MHC class II molecules by directing them to endocytic compartments, preventing peptide binding in the ER, and contributing to peptide “editing” in the MHC class II compartment. CD74 has recently been shown to have a role as an accessory signaling molecule because of its high- affinity binding to the pro-inflammatory cytokine macrophage migration inhibitory factor (MIF).

Plays a critical role in MHC class II antigen processing by stabilizing peptide-free class II alpha/beta heterodimers in a complex soon after their synthesis and directing transport of the complex from the endoplasmic reticulum to the endosomal/lysosomal system where the antigen processing and binding of antigenic peptides to MHC class II takes place. Serves as cell surface receptor for the cytokine MIF.; [Class-II-associated invariant chain peptide]: Binds to the peptide-binding site of MHC class II alpha/beta heterodimers forming an alpha-beta-CLIP complex, thereby preventing the loading of antigenic peptides to the MHC class II complex until its release by HLA-DM in the endosome. {PubMed:1448172}.; [Isoform p41]: Stabilizes the conformation of mature CTSL by binding to its active site and serving as a chaperone to help maintain a pool of mature enzyme in endocytic compartments and extracellular space of antigen-presenting cells (APCs). Has antiviral activity by stymieing the endosomal entry of Ebola virus and coronaviruses, including SARS-CoV-2 (PubMed:32855215). Disrupts cathepsin-mediated Ebola virus glycoprotein processing, which prevents viral fusion and entry. This antiviral activity is specific to p41 isoform (PubMed:32855215). {UniProtKB:P04441, PubMed:32855215}.

Dilute in PBS or medium which is identical to that used in the assay system.

This antibody is stable for at least one (1) year at -20C. Avoid repeated freezing and thawing.

这抗体识别人类CD74

qed-bioscience-anti-cd74-antibody-56451-1.png

免疫细胞化学

qed-bioscience-anti-cd74-antibody-56451-2.png

免疫细胞化学

qed-bioscience-anti-cd74-antibody-56451-3.png

免疫组化

qed-bioscience-anti-cd74-antibody-56451-4.png

Western Blot analysis of Human N87 cell lysates showing detection of CD74 protein using Mouse Anti-CD74 Monoclonal Antibody, Clone PIN 1.1 (56451). Primary Antibody: Mouse Anti-CD74 Monoclonal Antibody (56451) at 1:1000. Lysates treated with macrophage inhibitory factor (MIF). Courtesy of: Victor E. Reyes, University of Texas Medical Branch, USA.

免疫印迹