产品简要
公司名称 :
QED Bioscience
产品类型 :
抗体
产品名称 :
抗PSD95抗体
目录 :
56457
规格 :
100微克
价格 :
427美元
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
7E3-1B8
反应物种 :
小鼠, 大鼠,
更多信息或购买 :
图像
图像 1 :
QED Bioscience 56457 图像 1
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-PSD95 Monoclonal Antibody, Clone 7E3 (56457). Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20°C. Primary Antibody: Mouse Anti-PSD95 Monoclonal Antibody (56457) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Filamentous-like staining.
图像 2 :
QED Bioscience 56457 图像 2
Immunohistochemistry analysis using Mouse Anti-PSD95 Monoclonal Antibody, Clone 7E3 (56457). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-PSD95 Monoclonal Antibody (56457) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Basal cell staining in the epidermis, some hair follicle staining, dermal staining. Backskin obtained from transgenic mice.
图像 3 :
QED Bioscience 56457 图像 3
Immunohistochemistry analysis using Mouse Anti-PSD95 Monoclonal Antibody, Clone 7E3 (56457). Tissue: Neocortex. Species: Rat. Primary Antibody: Mouse Anti-PSD95 Monoclonal Antibody (56457) at 1:1000.
产品信息
目录号 :
56457
产品名称 :
抗PSD95抗体
NAME+SKU :
抗PSD95抗体(56457)
产品_TYPE :
抗体
描述 :
抗PSD95小鼠单克隆抗体
SPECIFICITY SHORT :
PSD95
物种活性 :
小鼠, 大鼠, 牛
靶标名 :
PSD95
UniProt数据库 :
P31016
LOCATION :
Cell membrane , Cell junction, synapse, postsynaptic density , Cell junction, synapse , Cytoplasm , Cell projection, axon , Cell projection, dendritic spine , Cell projection, dendrite , Cell junction, synapse, presynapse
蛋白名 :
Disks large homolog 4
别称 :
Postsynaptic density protein 95, PSD-95, Synapse-associated protein 90, SAP-90, SAP90
基因名称 :
Dlg4
克隆性 :
单克隆
形式 :
液体
浓度 :
Lot Specific
规格1 :
100微克
标价规格1 :
427美元
DISTIRBUTOR价格 :
298.9
SKU号规格1 :
56457
应用 :
免疫印迹
应用INSTRUCTIONS :
Immunoblotting: use at 1ug/ml. A band of ~100 kDa is detected. Immunocytochemistry: use at 1-10ug/ml. Immunofluorescence: use at10ug/ml. These are recommended concentrations. Endusers should determine optimal concentrations for their application.
宿主 :
小鼠
格式 :
纯化
纯化 :
Purified by Protein G affinity chromatography.
抗体亚型 :
IgG2a
克隆编号 :
7E3-1B8
免疫原 :
重组大鼠PSD95
研究领域 :
神经科学
配方 :
PBS, pH值7.4, 50% 甘油, 0.09% 叠氮化钠
缓冲液 :
磷酸盐缓冲液
缓冲液pH值 :
pH值7.4
缓冲液抗MICROBIAL :
0.09% 叠氮化钠
缓冲液CRYOPRESERVATIVE :
50% 甘油
靶标背景 :
Postsynaptic Density Protein 95 (PSD95), also known as Synapse Associated Protein 90kDa, is a member of the membrane-associated guanylate kinase family of proteins. PSD95 is a scaffolding protein involved in the assembly and function of the postsynaptic density complex. This family of proteins consists of N-terminal variable segments followed by three amino-terminal PDZ domains, an upstream SH3 domain, and an inactive carboxy-terminal guanylate kinase domain. The first and second PDZ domains localize NMDA receptors and K+ channels to synapses, and the third binds to neuroligins, neuronal cell adhesion molecules. PSD95 also binds to neuronal nitric oxide synthase. PSD95 is implicated in experience-dependent plasticity and plays an indispensable role in learning. Mutations in PSD95 are associated with autism.
FUNCTION :
Postsynaptic scaffolding protein that plays a critical role in synaptogenesis and synaptic plasticity by providing a platform for the postsynaptic clustering of crucial synaptic proteins (PubMed:15317815, PubMed:15358863, PubMed:19596852, PubMed:23300088, PubMed:26679993). Interacts with the cytoplasmic tail of NMDA receptor subunits and shaker-type potassium channels. Required for synaptic plasticity associated with NMDA receptor signaling. Overexpression or depletion of DLG4 changes the ratio of excitatory to inhibitory synapses in hippocampal neurons. May reduce the amplitude of ASIC3 acid-evoked currents by retaining the channel intracellularly. May regulate the intracellular trafficking of ADR1B (By similarity). Also regulates AMPA-type glutamate receptor (AMPAR) immobilization at postsynaptic density keeping the channels in an activated state in the presence of glutamate and preventing synaptic depression (PubMed:19596852).Under basal conditions, cooperates with FYN to stabilize palmitoyltransferase ZDHHC5 at the synaptic membrane through FYN-mediated phosphorylation of ZDHHC5 and its subsequent inhibition of association with endocytic proteins (By similarity). {UniProtKB:P78352, UniProtKB:Q62108, PubMed:15317815, PubMed:15358863, PubMed:19596852, PubMed:23300088, PubMed:26679993}.
稀释INSTRUCTIONS :
Dilute in PBS or medium which is identical to that used in the assay system.
STORAGE空调 :
This antibody is stable for 1 month at 4°C or one year at -20°C. Avoid multiple freeze-thaw cycles.
特异性 :
这抗体识别小鼠, 大鼠, 猪, 和人类PSD95
图像Name_1 :
qed-bioscience-anti-psd95-antibody-56457-1.png
应用1 :
免疫细胞化学
图像Name_2 :
qed-bioscience-anti-psd95-antibody-56457-2.png
应用2 :
免疫组化
图像Name_3 :
qed-bioscience-anti-psd95-antibody-56457-3.png
应用3 :
免疫组化
图像Name_4 :
qed-bioscience-anti-psd95-antibody-56457-4.png
图像Caption_4 :
Western Blot analysis of Rat brain membrane lysate showing detection of PSD95 protein using Mouse Anti-PSD95 Monoclonal Antibody, Clone 7E3 (56457). Primary Antibody: Mouse Anti-PSD95 Monoclonal Antibody (56457) at 1:1000.
应用4 :
免疫印迹
更多信息或购买 :
公司信息
QED Bioscience
10919 Technology Place, Suite C San Diego, CA 92127
info@qedbio.com
http://www.qedbio.com
800-929-2114 858-675-2405
公司总部: 美国