产品简要
公司名称 :
QED Bioscience
产品类型 :
抗体
产品名称 :
Anti-pan-Shank Antibody
目录 :
56468
规格 :
100微克
价格 :
436美元
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
S23b-49
反应物种 :
人类, 小鼠, 大鼠
更多信息或购买 :
QED Bioscience 产品网页
图像
图像 1 :
QED Bioscience 56468 图像 1
Western Blot analysis of Rat brain membrane lysate showing detection of SHANK protein using Mouse Anti-SHANK Monoclonal Antibody, Clone S23b-49 (56468). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-SHANK Monoclonal Antibody (56468) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
图像 2 :
QED Bioscience 56468 图像 2
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-SHANK (pan) Monoclonal Antibody, Clone S23b-49 (56468). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-SHANK (pan) Monoclonal Antibody (56468) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm . Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) SHANK (pan) Antibody. (D) Composite.
图像 3 :
QED Bioscience 56468 图像 3
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-SHANK (pan) Monoclonal Antibody, Clone S23b-49 (56468). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-SHANK (pan) Monoclonal Antibody (56468) at 1:50 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) SHANK (pan) Antibody (D) Composite.
产品信息
目录号 :
56468
产品名称 :
Anti-pan-Shank Antibody
NAME+SKU :
Anti-pan-Shank Antibody (56468)
产品_TYPE :
抗体
描述 :
Anti-pan-Shank Mouse Monoclonal Antibody
SPECIFICITY SHORT :
pan-Shank
物种活性 :
人类, 小鼠, 大鼠
靶标名 :
pan-Shank
UniProt数据库 :
Q9QX74
LOCATION :
Apical cell membrane , Cytoplasm , Cell junction, synapse , Cell junction, synapse, postsynaptic density , Cell projection, growth cone , Cell projection, dendritic spine
蛋白名 :
SH3和多个锚蛋白重复区域蛋白2
别称 :
Shank2, Cortactin-binding protein 1, CortBP1, GKAP/SAPAP-interacting protein, Proline-rich synapse-associated protein 1, ProSAP1, SPANK-3
基因名称 :
Shank2
克隆性 :
单克隆
形式 :
液体
浓度 :
Lot Specific
规格1 :
100微克
标价规格1 :
436美元
DISTIRBUTOR价格 :
305.2
SKU号规格1 :
56468
应用 :
免疫印迹, 免疫组化, 免疫细胞化学, IF
应用INSTRUCTIONS :
Immunoblotting: use at 1-10ug/ml. A band of ~160kDa is detected. Immunohistochemistry and Immunocytochemistry: use at 0.1-1ug/ml Immunofluorescence: use at 1-10ug/ml These are recommended concentrations. User should determine optimal concentrations for their application. Positive control: Adult rat brain lysate.
宿主 :
小鼠
格式 :
纯化
纯化 :
经免疫亲和层析纯化
抗体亚型 :
IgG1
克隆编号 :
S23b-49
免疫原 :
Fusion protein corresponding to aa 84-309 (SH3/PDZ domains) of rat Shank2 (accession no. Q9QX74). This sequence is 94
研究领域 :
神经科学
配方 :
PBS, pH值7.4;50% 甘油, 0.09% 叠氮化钠
缓冲液 :
磷酸盐缓冲液
缓冲液pH值 :
pH值7.4
缓冲液抗MICROBIAL :
0.09% 叠氮化钠
缓冲液CRYOPRESERVATIVE :
50% 甘油
靶标背景 :
Shank proteins are a family of scaffold proteins identified through their interaction with a variety of membrane and cyto- plasmic proteins. Shank proteins at postsynaptic sites of excitatory synapses play roles in signal transmission into the postsynaptic neuron. Shank proteins are crucial in receptor tyrosine kinase signaling. Recent studies suggest that disruption of glutamate receptors at the Shank-postsynaptic platform could contribute to the destruction of post- synaptic density which underlies synaptic dysfunction and loss in Alzheimer’s disease.
FUNCTION :
Seems to be an adapter protein in the postsynaptic density (PSD) of excitatory synapses that interconnects receptors of the postsynaptic membrane including NMDA-type and metabotropic glutamate receptors, and the actin-based cytoskeleton. May play a role in the structural and functional organization of the dendritic spine and synaptic junction. {PubMed:10506216}.
稀释INSTRUCTIONS :
Dilute in PBS or medium which is identical to that used in the assay system.
STORAGE空调 :
This antibody is stable for at least one (1) year at -20C. Avoid repeated freezing and thawing.
特异性 :
This antibody recognizes human, mouse, and rat Shank 1, 2, and 3.
图像Name_1 :
qed-bioscience-anti-pan-shank-antibody-56468-1.png
应用1 :
免疫印迹
图像Name_2 :
qed-bioscience-anti-pan-shank-antibody-56468-2.png
应用2 :
免疫细胞化学
图像Name_3 :
qed-bioscience-anti-pan-shank-antibody-56468-3.png
应用3 :
免疫细胞化学
更多信息或购买 :
QED Bioscience 产品网页
公司信息
QED Bioscience
10919 Technology Place, Suite C San Diego, CA 92127
info@qedbio.com
http://www.qedbio.com
800-929-2114 858-675-2405
公司总部: 美国