产品简要
公司名称 :
QED Bioscience
产品类型 :
抗体
产品名称 :
Anti-Shank 1 Antibody
目录 :
56469
规格 :
100微克
价格 :
436美元
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
S22-21
反应物种 :
人类, 小鼠, 大鼠
更多信息或购买 :
QED Bioscience 产品网页
图像
图像 1 :
QED Bioscience 56469 图像 1
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-SHANK1 Monoclonal Antibody, Clone S22-21 (56469). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-SHANK1 Monoclonal Antibody (56469) at 1:50 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) SHANK1 Antibody (D) Composite.
图像 2 :
QED Bioscience 56469 图像 2
Immunohistochemistry analysis using Mouse Anti-SHANK1 Monoclonal Antibody, Clone S22-21 (56469). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-SHANK1 Monoclonal Antibody (56469) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Filaggrin-like staining (upper layer aggregations of staining).
图像 3 :
QED Bioscience 56469 图像 3
Western Blot analysis of Rat brain membrane lysate showing detection of SHANK1 protein using Mouse Anti-SHANK1 Monoclonal Antibody, Clone S22-21 (56469). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-SHANK1 Monoclonal Antibody (56469) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
产品信息
目录号 :
56469
产品名称 :
Anti-Shank 1 Antibody
NAME+SKU :
Anti-Shank 1 Antibody (56469)
产品_TYPE :
抗体
描述 :
抗Shank1小鼠单克隆抗体
SPECIFICITY SHORT :
Shank1
物种活性 :
人类, 小鼠, 大鼠
靶标名 :
Shank 1
UniProt数据库 :
Q9WV48
LOCATION :
Cytoplasm , Cell junction, synapse , Cell junction, synapse, postsynaptic density
蛋白名 :
SH3和多个锚蛋白重复区域蛋白1
别称 :
Shank1, GKAP/SAPAP-interacting protein, SPANK-1, Somatostatin receptor-interacting protein, SSTR-interacting protein, SSTRIP, Synamon
基因名称 :
Shank1
克隆性 :
单克隆
形式 :
液体
浓度 :
Lot Specific
规格1 :
100微克
标价规格1 :
436美元
DISTIRBUTOR价格 :
305.2
SKU号规格1 :
56469
应用 :
免疫印迹, 免疫组化, 免疫细胞化学, IF
应用INSTRUCTIONS :
Immunoblotting: use at 1-10ug/ml. Bands of ~190-220kDa (alternative splice variants) are detected. Immunohistochemistry and Immunocytochemistry: use at 0.1-1ug/ml Immunofluorescence: use at 1-10ug/ml These are recommended concentrations. User should determine optimal concentrations for their application. Positive control: Adult rat brain.
宿主 :
小鼠
格式 :
纯化
纯化 :
经免疫亲和层析纯化
抗体亚型 :
IgG1
克隆编号 :
S22-21
免疫原 :
Fusion protein corresponding to aa 469-691 (SH3/PDZ domains) of rat Shank1 (accession no. Q9WV48). This sequence is 96
研究领域 :
神经科学
配方 :
PBS, pH值7.4;50% 甘油, 0.09% 叠氮化钠
缓冲液 :
磷酸盐缓冲液
缓冲液pH值 :
pH值7.4
缓冲液抗MICROBIAL :
0.09% 叠氮化钠
缓冲液CRYOPRESERVATIVE :
50% 甘油
靶标背景 :
Shank proteins are a family of scaffold proteins identified through their interaction with a variety of membrane and cyto- plasmic proteins. Shank proteins at postsynaptic sites of excitatory synapses play roles in signal transmission into the postsynaptic neuron. Shank proteins are crucial in receptor tyrosine kinase signaling. Recent studies suggest that disruption of glutamate receptors at the Shank-postsynaptic platform could contribute to the destruction of post- synaptic density which underlies synaptic dysfunction and loss in Alzheimer’s disease. Shank1 might be relevant to human autism spectrum disorders due to its differential role in specific cognitive processes along with its importance in synapse structure and function.
FUNCTION :
Seems to be an adapter protein in the postsynaptic density (PSD) of excitatory synapses that interconnects receptors of the postsynaptic membrane including NMDA-type and metabotropic glutamate receptors, and the actin-based cytoskeleton. Plays a role in the structural and functional organization of the dendritic spine and synaptic junction. Overexpression promotes maturation of dendritic spines and the enlargement of spine heads via its ability to recruit Homer to postsynaptic sites, and enhances presynaptic function. {PubMed:11498055, PubMed:18287537}.
稀释INSTRUCTIONS :
Dilute in PBS or medium which is identical to that used in the assay system.
STORAGE空调 :
This antibody is stable for at least one (1) year at -20C. Avoid repeated freezing and thawing.
特异性 :
This antibody recognizes human, mouse, and rat Shank 1. It does not react with Shank2 or 3.
图像Name_1 :
qed-bioscience-anti-shank-1-antibody-56469-1.png
应用1 :
免疫细胞化学
图像Name_2 :
qed-bioscience-anti-shank-1-antibody-56469-2.png
应用2 :
免疫组化
图像Name_3 :
qed-bioscience-anti-shank-1-antibody-56469-3.png
应用3 :
免疫印迹
更多信息或购买 :
QED Bioscience 产品网页
公司信息
QED Bioscience
10919 Technology Place, Suite C San Diego, CA 92127
info@qedbio.com
http://www.qedbio.com
800-929-2114 858-675-2405
公司总部: 美国