产品简要
公司名称 :
QED Bioscience
产品类型 :
抗体
产品名称 :
抗视紫红质抗体
目录 :
56474
规格 :
100微克
价格 :
436美元
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
1D4
反应物种 :
牛
更多信息或购买 :
QED Bioscience 产品网页
图像
图像 1 :
QED Bioscience 56474 图像 1
Immunohistochemistry analysis using Mouse Anti-Rhodopsin Monoclonal Antibody, Clone 1D4 (56474). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Rhodopsin Monoclonal Antibody (56474) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Dull epidermal staining.
图像 2 :
QED Bioscience 56474 图像 2
Western Blot analysis of Human Cell lysates showing detection of Rhodopsin protein using Mouse Anti-Rhodopsin Monoclonal Antibody, Clone 1D4 (56474). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Rhodopsin Monoclonal Antibody (56474) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
产品信息
目录号 :
56474
产品名称 :
抗视紫红质抗体
NAME+SKU :
抗视紫红质抗体(56474)
产品_TYPE :
抗体
描述 :
抗视紫红质小鼠单克隆抗体
SPECIFICITY SHORT :
视紫红质
物种活性 :
Vertebrate
靶标名 :
视紫红质
UniProt数据库 :
P02699
LOCATION :
Membrane , Cell projection, cilium, photoreceptor outer segment
蛋白名 :
视紫红质
基因名称 :
RHO
克隆性 :
单克隆
形式 :
液体
浓度 :
Lot Specific
规格1 :
100微克
标价规格1 :
436美元
DISTIRBUTOR价格 :
305.2
SKU号规格1 :
56474
应用 :
免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀
应用INSTRUCTIONS :
Immunoblotting: use at 1-10ug/ml. A band of ~40kDa is detected. Immunohistochemistry/immunocyto- chemistry: use at 1-10ug/ml Immunoprecipitation: use at 1-2ug per 100-500ug of protein. These are recommended concentrations. User should determine optimal concentrations for their application. Positive control: Rat eye lysate
宿主 :
小鼠
格式 :
纯化
纯化 :
经免疫亲和层析纯化
抗体亚型 :
IgG1
克隆编号 :
1D4
免疫原 :
牛视紫红质
研究领域 :
神经科学
配方 :
PBS, pH值7.4;50% 甘油, 0.09% 叠氮化钠
缓冲液 :
磷酸盐缓冲液
缓冲液pH值 :
pH值7.4
缓冲液抗MICROBIAL :
0.09% 叠氮化钠
缓冲液CRYOPRESERVATIVE :
50% 甘油
靶标背景 :
Rhodopsin is comprised of the protein opsin and a reversibly covalently bound cofactor, retinal. Opsin, made up of seven membrane-embedded alpha helices, binds retinal, a photoreactive chromophore, in a central pocket. In addition to being the pigment of the retina that is responsible for the formation of photoreceptor cells, rhodopsin conveys information stored in the chromophore to the surface of the molecule upon light absorption. Mutations in the rhodopsin gene lead to retinitis pigmentosa.
FUNCTION :
Photoreceptor required for image-forming vision at low light intensity. Required for photoreceptor cell viability after birth (By similarity). Light-induced isomerization of 11-cis to all-trans retinal triggers a conformational change that activates signaling via G-proteins (PubMed:10926528, PubMed:12044163, PubMed:11972040, PubMed:16908857, PubMed:16586416, PubMed:17060607, PubMed:17449675, PubMed:18818650, PubMed:21389983, PubMed:22198838, PubMed:23579341, PubMed:25205354, PubMed:27458239). Subsequent receptor phosphorylation mediates displacement of the bound G-protein alpha subunit by the arrestin SAG and terminates signaling (PubMed:1396673, PubMed:15111114). {UniProtKB:P08100, PubMed:1396673, PubMed:16586416, PubMed:16908857, PubMed:17060607, PubMed:17449675, PubMed:18818650, PubMed:21389983, PubMed:22198838, PubMed:23579341, PubMed:25205354, PubMed:27458239, PubMed:10926528, PubMed:11972040, PubMed:12044163, PubMed:15111114, PubMed:26526852}.
稀释INSTRUCTIONS :
Dilute in PBS or medium which is identical to that used in the assay system.
STORAGE空调 :
This antibody is stable for 1 month at 4°C or one year at -20°C. Avoid multiple freeze-thaw cycles.
特异性 :
This antibody recognizes the C-terminal aa TETSQVAPA(COOH) of native and recombinant vertebrate rhodopsin.
图像Name_1 :
qed-bioscience-anti-rhodopsin-antibody-56474-1.png
应用1 :
免疫组化
图像Name_2 :
qed-bioscience-anti-rhodopsin-antibody-56474-2.png
应用2 :
免疫印迹
更多信息或购买 :
QED Bioscience 产品网页
公司信息
QED Bioscience
10919 Technology Place, Suite C San Diego, CA 92127
info@qedbio.com
http://www.qedbio.com
800-929-2114 858-675-2405
公司总部: 美国