产品简要
公司名称 :
QED Bioscience
产品类型 :
抗体
产品名称 :
抗GFAP抗体
目录 :
56566
规格 :
100微克
价格 :
436美元
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
S206A-8
反应物种 :
人类, 小鼠, 大鼠
更多信息或购买 :
图像
图像 1 :
QED Bioscience 56566 图像 1
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GFAP Monoclonal Antibody, Clone S206A-8 (56566). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-GFAP Monoclonal Antibody (56566) at 1:50 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) GFAP Antibody (D) Composite.
图像 2 :
QED Bioscience 56566 图像 2
Western Blot analysis of Rat Brain Membrane showing detection of GFAP protein using Mouse Anti-GFAP Monoclonal Antibody, Clone S206A-8 (56566). Primary Antibody: Mouse Anti-GFAP Monoclonal Antibody (56566) at 1:250.
图像 3 :
QED Bioscience 56566 图像 3
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GFAP Monoclonal Antibody, Clone S206A-8 (56566). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-GFAP Monoclonal Antibody (56566) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm . Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) GFAP Antibody. (D) Composite.
产品信息
目录号 :
56566
产品名称 :
抗GFAP抗体
NAME+SKU :
抗GFAP抗体(56566)
产品_TYPE :
抗体
描述 :
抗GFAP小鼠单克隆抗体
SPECIFICITY SHORT :
GFAP
物种活性 :
人类, 小鼠, 大鼠
靶标名 :
GFAP
UniProt数据库 :
P14136
LOCATION :
细胞质
蛋白名 :
胶质纤维酸性蛋白
别称 :
GFAP
基因名称 :
GFAP
克隆性 :
单克隆
形式 :
液体
浓度 :
1.0毫克/毫升
规格1 :
100微克
标价规格1 :
436美元
DISTIRBUTOR价格 :
305.2
SKU号规格1 :
56566
应用 :
免疫印迹, IF
应用INSTRUCTIONS :
Immunoblotting: use at 1-4ug/ml. A band of ~50kDa is detected. Immunofluorescence: use at 10ug/ml. These are recommended concentrations. Endusers should determine optimal concentrations for their application.
宿主 :
小鼠
格式 :
纯化
纯化 :
Purified by Protein G affinity chromatography.
抗体亚型 :
IgG1
克隆编号 :
S206A-8
免疫原 :
Synthetic peptide corresponding to aa 411-422 (KTVEMRDGEVIK) of human GFAP. This sequence is 100
研究领域 :
神经科学
配方 :
PBS, pH值7.4, 0.1% 叠氮化钠, 50% 甘油
缓冲液 :
磷酸盐缓冲液
缓冲液pH值 :
pH值7.4
缓冲液抗MICROBIAL :
0.1% 叠氮化钠
缓冲液CRYOPRESERVATIVE :
50% 甘油
靶标背景 :
Glial fibrillary acidic protein (GFAP) is a major structural component of astrocytes. Phosphorylation of GFAP, and its association with annexin II-p2 and S-100, regulates GFAP polymerization. One of the first events that occurs during astrocyte proliferation is increased GFAP expression. Antibodies to GFAP have been detected in individuals with dementia, although the significance of this has not been conclusively determined.
FUNCTION :
GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
稀释INSTRUCTIONS :
Dilute in PBS or medium that is identical to that used in the assay system.
STORAGE空调 :
This product is stable for at least one (1) year at -20C.
特异性 :
This antibody recognizes human, mouse and rat GFAP. It cross-reacts with GFAP- R416W and other GFAP mutant proteins.
图像Name_1 :
qed-bioscience-anti-gfap-antibody-56566-1.png
应用1 :
免疫细胞化学
图像Name_2 :
qed-bioscience-anti-gfap-antibody-56566-2.png
应用2 :
免疫印迹
图像Name_3 :
qed-bioscience-anti-gfap-antibody-56566-3.png
应用3 :
免疫细胞化学
更多信息或购买 :
公司信息
QED Bioscience
10919 Technology Place, Suite C San Diego, CA 92127
info@qedbio.com
http://www.qedbio.com
800-929-2114 858-675-2405
公司总部: 美国