产品简要
公司名称 :
QED Bioscience
产品类型 :
抗体
产品名称 :
抗beta肌动蛋白抗体
目录 :
56574
规格 :
100微克
价格 :
436美元
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
反应物种 :
人类
更多信息或购买 :
图像
图像 1 :
QED Bioscience 56574 图像 1
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Beta Actin Polyclonal Antibody (56574). Tissue: Cervical cancer cell line (HeLa). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-Beta Actin Polyclonal Antibody (56574) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Cytoplasm, Cytoskeleton. Magnification: 40X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Beta Actin Antibody (D) Composite.
图像 2 :
QED Bioscience 56574 图像 2
Western blot analysis of Human HeLa and HEK293T cell lysates showing detection of ~41.7kDa beta Actin protein using Rabbit Anti-beta Actin Polyclonal Antibody (56574). Lane 1: MW Ladder. Lane 2: Human HeLa (20 µg). Lane 3: Human 293T (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-beta Actin Polyclonal Antibody (56574) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~41.7kDa.
产品信息
目录号 :
56574
产品名称 :
抗beta肌动蛋白抗体
NAME+SKU :
抗beta肌动蛋白抗体(56574)
产品_TYPE :
抗体
描述 :
抗beta肌动蛋白兔多克隆抗体
SPECIFICITY SHORT :
beta肌动蛋白
物种活性 :
人类
靶标名 :
beta肌动蛋白
UniProt数据库 :
P60709
LOCATION :
Cytoplasm, cytoskeleton , Nucleus
蛋白名 :
Actin, cytoplasmic 1
别称 :
Beta-actin [Cleaved into: Actin, cytoplasmic 1, N-terminally processed]
基因名称 :
ACTB
克隆性 :
多克隆
形式 :
液体
浓度 :
1.0毫克/毫升
规格1 :
100微克
标价规格1 :
436美元
DISTIRBUTOR价格 :
305.2
SKU号规格1 :
56574
应用 :
免疫印迹, IF
应用INSTRUCTIONS :
Immunoblotting: use at 1ug/ml. Immunofluorescence: use at 10ug/ml. These are recommended concentrations; endusers should determine optimal concentrations for their applications.
宿主 :
格式 :
纯化
纯化 :
Purified by immuno-affinity chromatography on the peptide immunogen.
免疫原 :
Synthetic peptide corresponding to mid-protein amino acids of human beta-actin.
研究领域 :
神经科学
配方 :
PBS, pH值7.4, 50% 甘油, 0.09% 叠氮化钠
缓冲液 :
磷酸盐缓冲液
缓冲液pH值 :
pH值7.4
缓冲液抗MICROBIAL :
0.09% 叠氮化钠
缓冲液CRYOPRESERVATIVE :
50% 甘油
靶标背景 :
Actins are essential components of the cytoskeleton with critical roles in a wide range of cellular processes including cell migration, cell division, and the regulation of gene expression. Beta-actin, product of the Actb gene, is one of six different actin isoforms and is one of the two nonmuscle cytoplasmic actins (gamma-actin is the other); both are ubiquitously expressed and highly conserved from birds to mammals. Mutations in the Actb gene have been associated with diffuse large B-cell lymphoma. Beta-actin (molecular weight ~42kDa) is often used as a loading control in Western blots.
FUNCTION :
Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells (PubMed:29581253). Actin exists in both monomeric (G-actin) and polymeric (F-actin) forms, both forms playing key functions, such as cell motility and contraction (PubMed:29581253). In addition to their role in the cytoplasmic cytoskeleton, G- and F-actin also localize in the nucleus, and regulate gene transcription and motility and repair of damaged DNA (PubMed:29925947). {PubMed:29581253, PubMed:29925947}.
稀释INSTRUCTIONS :
Dilute in PBS or medium that is identical to that used in the assay system.
STORAGE空调 :
This antibody is stable for at least one (1) year at -20C.
特异性 :
这抗体识别人类beta肌动蛋白
图像Name_1 :
qed-bioscience-anti-beta-actin-antibody-56574-1.png
应用1 :
免疫细胞化学
图像Name_2 :
qed-bioscience-anti-beta-actin-antibody-56574-2.png
应用2 :
免疫印迹
更多信息或购买 :
公司信息
QED Bioscience
10919 Technology Place, Suite C San Diego, CA 92127
info@qedbio.com
http://www.qedbio.com
800-929-2114 858-675-2405
公司总部: 美国