抗体

抗TATA结合蛋白抗体

70102

200微克

408美元

单克隆

小鼠

未共轭

1TBP18

人类, 小鼠, 大鼠

抗TATA结合蛋白抗体

抗TATA结合蛋白抗体(70102)

抗体

抗TATA-结合蛋白小鼠单克隆抗体

TATA-结合蛋白

人类, 小鼠, 大鼠

TATA结合蛋白

P20226

Nucleus

TATA盒蛋白

TATA sequence-binding protein, TATA-binding factor, TATA-box factor, Transcription initiation factor TFIID TBP subunit

TBP

单克隆

液体

Lot Specific

200微克

408美元

285.6

70102

WB, IP, Gel Shift

This antibody may be used in Western blot and electrophoretic mobility (gel shift) assays to detect mammalian TBP. It may also be used to immunoprecipitate a truncated TBP molecule containing only the N-terminal domain.

小鼠

纯化

Purified by Protein G affinity chromatography.

IgG1

1TBP18

重组人类TBP

蛋白转录

PBS, pH值7.4

磷酸盐缓冲液

pH值7.4

The TATA-binding protein (TBP) plays a central role in the assembly of most eukaryotic transcription initiation complexes. TBP assembles with other proteins to form unique multimeric complexes for each of the three different nuclear RNA polymerases. TBP has been cloned from a variety of species and consists of two distinct domains. The C-terminal domain (~180 amino acids) is highly conserved among species, whereas the N-terminal domain varies considerably in length and sequence among different species. The conserved C-terminal domain contains the DNA-binding region as well as regions that interact with positive and negative regulatory proteins. In human TBP, the non-conserved N-terminal domain includes a stretch of glutamine residues ranging from 26 to 42. Several research groups have found an association between expansion of this polyglutamine tract with rare forms of spinocerebellar ataxia and other neurodegenerative diseases including Huntington’s disease.

General transcription factor that functions at the core of the DNA-binding multiprotein factor TFIID (PubMed:2374612, PubMed:2363050, PubMed:2194289, PubMed:9836642, PubMed:27193682). Binding of TFIID to the TATA box is the initial transcriptional step of the pre-initiation complex (PIC), playing a role in the activation of eukaryotic genes transcribed by RNA polymerase II (PubMed:2374612, PubMed:2363050, PubMed:2194289, PubMed:9836642, PubMed:27193682). Component of a BRF2-containing transcription factor complex that regulates transcription mediated by RNA polymerase III (PubMed:26638071). Component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC (pre-initiation complex) during RNA polymerase I-dependent transcription (PubMed:15970593). The rate of PIC formation probably is primarily dependent on the rate of association of SL1 with the rDNA promoter. SL1 is involved in stabilization of nucleolar transcription factor 1/UBTF on rDNA. {PubMed:15970593, PubMed:2194289, PubMed:2363050, PubMed:2374612, PubMed:26638071, PubMed:27193682, PubMed:9836642, ECO:0000305}.

Dilute in PBS or medium which is identical to that used in the assay system.

These antibodies are stable for at least one (1) year at -20C to -70C. Store product in appropriate aliquots to avoid multiple freeze-thaw cycles.

This antibody recognizes an epitope within amino acid residues 1-20 of human, mouse and rat TBP. It does not react with TBP from Drosophila, yeast, silk worm, or Xenopus. Other species not investigated.

qed-bioscience-anti-tata-binding-protein-antibody-70102-1.jpg

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