小鼠 单克隆 (D01)
反应物种: 人类
反应物种: 人类
Untreated (–) and treated (+) HCT116 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 antibody [DO1] (3339) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Immunoprecipitation of p53 protein from 293T whole cell extracts using 5 ug of p53 antibody [DO1] (3339). Western blot analysis was performed using p53 antibody [D01] (3339). EasyBlot anti-Mouse IgG was used as a secondary reagent.
Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 antibody [DO1] (3339) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
规格: 100微克
价格: 461美元
至产商
小鼠 单克隆 (240)
反应物种: 人类
反应物种: 人类
Sample (30 ug of whole cell lysate) A: HCT116 cells with mock treatment for 24 hr B: HCT116 cells with 30 uM cisplatin treatment for 24 hr 10% SDS PAGE 3340 diluted at 1:1000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using p53(3340) antibody at 1:200 dilution.
Sample (30 ug of whole cell lysate) A: 293T B: A431 10% SDS PAGE 3340 diluted at 1:1000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
规格: 100微克
价格: 461美元
至产商
domestic rabbit 多克隆
反应物种: 人类
反应物种: 人类
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-p53 Polyclonal Antibody (3347). Tissue: Cervical cancer cell line (HeLa). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-p53 Polyclonal Antibody (3347) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Cytoplasm, PML body, Endoplasmic Reticulum. Magnification: 40X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) p53 Antibody (D) Composite.
Western blot analysis of Human HeLa and HEK293T cell lysates showing detection of ~43.7kDa p53 protein using Rabbit Anti-p53 Polyclonal Antibody (3347). Lane 1: MW Ladder. Lane 2: Human HeLa (20 µg). Lane 3: Human 293T (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-p53 Polyclonal Antibody (3347) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~43.7kDa.
Western blot analysis of Human A431 showing detection of ~43.7kDa p53 protein using Rabbit Anti-p53 Polyclonal Antibody (3347). Lane 1: MW Ladder. Lane 2: Human A431 (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-p53 Polyclonal Antibody (3347) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~43.7kDa.
规格: 100微克
价格: 436美元
至产商
小鼠 单克隆 (G053.1)
反应物种: 人类
反应物种: 人类
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