抗p53抗体
QED Bioscience
目录: 3347
domestic rabbit 多克隆
反应物种: 人类

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-p53 Polyclonal Antibody (3347). Tissue: Cervical cancer cell line (HeLa). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-p53 Polyclonal Antibody (3347) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Cytoplasm, PML body, Endoplasmic Reticulum. Magnification: 40X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) p53 Antibody (D) Composite.

Western blot analysis of Human HeLa and HEK293T cell lysates showing detection of ~43.7kDa p53 protein using Rabbit Anti-p53 Polyclonal Antibody (3347). Lane 1: MW Ladder. Lane 2: Human HeLa (20 µg). Lane 3: Human 293T (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-p53 Polyclonal Antibody (3347) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~43.7kDa.

Western blot analysis of Human A431 showing detection of ~43.7kDa p53 protein using Rabbit Anti-p53 Polyclonal Antibody (3347). Lane 1: MW Ladder. Lane 2: Human A431 (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-p53 Polyclonal Antibody (3347) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~43.7kDa.
规格: 100微克
价格: 436美元
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