Domestic guinea pig, 人类, 小鼠, 大鼠
免疫印迹, 免疫细胞化学, 免疫沉淀, 染色质免疫沉淀 , 免疫组化-冰冻切片
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Detection of Human HIF-1 alpha by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line untreated (-) or treated (+) with 150 ╡M CoCl2 for 16 hours. PVDF membrane was probed with 0.5 ╡g/mL of Goat Anti-Human/Mouse/Rat HIF-1 alpha Antigen-affinity Purified Polyclonal Antibody, followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109 ). A specific band was detected for HIF-1 alpha at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1 .
The Human/Mouse/Rat HIF-1 alpha Antibody from R&D Systems is a goat polyclonal antibody to HIF-1 alpha. This antibody reacts with human, mouse, rat. The Human/Mouse/Rat HIF-1 alpha Antibody has been validated for the following applications: Western Blot, Simple Western, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP), Immunocytochemistry, Knockout Validated.
ARNT-interacting protein, Basic-helix-loop-helix-PAS protein MOP1, BHLHE78, Class E basic helix-loop-helix protein 78, HIF 1A, HIF1 alpha, HIF-1 alpha, HIF-1a, HIF1A, HIF-1-alpha, HIF1-alpha, hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcriptionfactor), hypoxia-inducible factor 1-alpha, Member of PAS protein 1, member of PAS superfamily 1, MOP1HIF1-ALPHA, PAS domain-containing protein 8, PASD8alpha subunit (basic helix-loop-helix transcriptionfactor)
Lyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
E. coli -derived recombinant human HIF-1 alpha , Arg575-Asn826, Accession # Q16665.1
人类, 小鼠, 大鼠
Detects human, mouse and rat HIF-1 alpha .
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Western Blot, Simple Western, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP), Immunocytochemistry, Knockout Validated
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.