免疫印迹, 免疫组化, proximity ligation assay
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ER alpha/NR3A1 in Human Endometrial Cancer Tissue. ER alpha/NR3A1 was detected in immersion fixed paraffin-embedded sections of human endometrial cancer tissue using Sheep Anti-Human ER alpha/NR3A1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5715) at 10 ╡g/mL overnight at 4 ░C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013 ). Tissue was stained using the Anti-Sheep HRP-DAB Cell Tissue Staining Kit (brown; Catalog # CTS019 ) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections .
The Human ER alpha/NR3A1 Antibody from R&D Systems is a sheep polyclonal antibody to ER alpha/NR3A1. This antibody reacts with human. The Human ER alpha/NR3A1 Antibody has been validated for the following applications: Western Blot, Simple Western, Immunohistochemistry.
Lyophilized from a 0.2 ╡m filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 ╡m filtered solution in PBS.
E. coli -derived recombinant human ER alpha , Met1-Gln116, Accession # P03372
Detects human ER alpha
Western Blot, Simple Western, Immunohistochemistry
ER, ER alpha, Era, ER-alpha, ESR1, ESRESRA, Estradiol receptor, estrogen receptor, estrogen receptor 1, estrogen receptor alpha, estrogen receptor alpha delta 3*4,56,7*/819-2 isoform, estrogen receptor alpha delta 4 +49 isoform, estrogen receptor alpha delta 4*5,6,7*/654 isoform, NR3A1, NR3A1DKFZp686N23123, Nuclear receptor subfamily 3 group A member 1
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
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