Isayeva T, Chanda D, Kallman L, Eltoum I, Ponnazhagan S. Effects of sustained antiangiogenic therapy in multistage prostate cancer in TRAMP model. Cancer Res. 2007;67:5789-97 pubmed
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HIF-1 alpha in Human Kidney Cancer Tissue. HIF-1 alpha was detected in immersion fixed paraffin-embedded sections of human kidney cancer tissue using Mouse Anti-Human/Mouse HIF-1 alpha Monoclonal Antibody (Catalog # MAB1935) at 15 ╡g/mL overnight at 4 ░C. Tissue was stained using the Anti-Mouse HRP-DAB Cell Tissue Staining Kit (brown; Catalog # CTS002 ) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections .
The Human/Mouse HIF-1 alpha Antibody from R&D Systems is a mouse monoclonal antibody to HIF-1 alpha. This antibody reacts with human, mouse. The Human/Mouse HIF-1 alpha Antibody has been validated for the following applications: Immunohistochemistry, Intracellular Staining by Flow Cytometry, CyTOF-ready.
ARNT-interacting protein, Basic-helix-loop-helix-PAS protein MOP1, BHLHE78, Class E basic helix-loop-helix protein 78, HIF 1A, HIF1 alpha, HIF-1 alpha, HIF-1a, HIF1A, HIF-1-alpha, HIF1-alpha, hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcriptionfactor), hypoxia-inducible factor 1-alpha, Member of PAS protein 1, member of PAS superfamily 1, MOP1HIF1-ALPHA, PAS domain-containing protein 8, PASD8alpha subunit (basic helix-loop-helix transcriptionfactor)
Lyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
E. coli -derived recombinant human HIF-1 alpha , Arg575-Asn826, Accession # Q16665.1
Protein A or G purified from hybridoma culture supernatant
Recognizes human and mouse HIF-1 alpha in direct ELISAs.
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Details of Functionality :
ActivityAssaywCitation not found None None
Immunohistochemistry, Intracellular Staining by Flow Cytometry, CyTOF-ready
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