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Tau in Human Brain. Tau was detected in immersion fixed paraffin-embedded sections of human Alzheimer's brain using Mouse Anti-Tau Monoclonal Antibody (Catalog # MAB3494) at 15 ╡g/mL overnight at 4 ░C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013 ). Tissue was stained using the Anti-Mouse HRP-DAB Cell Tissue Staining Kit (brown; Catalog # CTS002 ) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies and processes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections .
The Tau Antibody from R&D Systems is a mouse monoclonal antibody to Tau. This antibody reacts with multi-species. The Tau Antibody has been validated for the following applications: Western Blot, Immunohistochemistry.
Lyophilized from a 0.2 ╡m filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 ╡m filtered solution in PBS.
E. coli -derived recombinant human Tau, Gln624-Gln756, Accession # P10636
Protein A or G purified from hybridoma culture supernatant
Detects human Tau in direct ELISAs and Western blots
DDPAC, FLJ31424, FTDP-17, G protein beta1/gamma2 subunit-interacting factor 1, MAPT, MGC138549, microtubule-associated protein tau, MSTD, MSTDMAPTL, MTBT1, MTBT1Neurofibrillary tangle protein, MTBT2, Neurofibrillary tangle protein, PHF-tau, PPND, tau, TAUPaired helical filament-tau
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.