产品简要
信息索取 :
公司名称 :
安迪生物R&D
产品类型 :
抗体
产品名称 :
人类/小鼠胱氨酸-天冬氨酸蛋白酶3抗体
目录 :
AF-605-NA
规格 :
100微克
价格 :
369美元
克隆性 :
多克隆
宿主 :
山羊
共轭标签 :
nonconjugated
反应物种 :
人类, 小鼠, 大鼠
应用 :
免疫印迹, 免疫沉淀
更多信息或购买 :
安迪生物R&D 产品网页
文章摘录数: 9
已发表的文献中的应用方法／种属／稀释度参考文献
  • 其他; 人类; 图 8
Pfister C, Pfrommer H, Tatagiba M, Roser F. Detection and quantification of apoptosis in primary cells using Taqman® protein assay. Methods Mol Biol. 2015;1219:57-73 pubmed 出版商
  • 免疫印迹; 人类
Koschny R, Ganten T, Sykora J, Haas T, Sprick M, Kolb A, et al. TRAIL/bortezomib cotreatment is potentially hepatotoxic but induces cancer-specific apoptosis within a therapeutic window. Hepatology. 2007;45:649-58 pubmed
Einsele-Scholz S, Malmsheimer S, Bertram K, Stehle D, Johänning J, Manz M, et al. Bok is a genuine multi-BH-domain protein that triggers apoptosis in the absence of Bax and Bak. J Cell Sci. 2016;129:2213-23 pubmed 出版商
Luyet C, Schulze K, Sayar B, Howald D, Muller E, Galichet A. Preclinical studies identify non-apoptotic low-level caspase-3 as therapeutic target in pemphigus vulgaris. PLoS ONE. 2015;10:e0119809 pubmed 出版商
Knievel J, Schulz W, Greife A, Hader C, Lübke T, Schmitz I, et al. Multiple mechanisms mediate resistance to sorafenib in urothelial cancer. Int J Mol Sci. 2014;15:20500-17 pubmed 出版商
Tian E, Landowski T, Stephens O, Yaccoby S, Barlogie B, Shaughnessy J. Ellipticine derivative NSC 338258 represents a potential new antineoplastic agent for the treatment of multiple myeloma. Mol Cancer Ther. 2008;7:500-9 pubmed 出版商
Chae J, Wood G, Masters S, Richard K, Park G, Smith B, et al. The B30.2 domain of pyrin, the familial Mediterranean fever protein, interacts directly with caspase-1 to modulate IL-1beta production. Proc Natl Acad Sci U S A. 2006;103:9982-7 pubmed
Deng J, Han Y, Yan C, Tian X, Tao J, Kang J, et al. Overexpressing cellular repressor of E1A-stimulated genes protects mesenchymal stem cells against hypoxia- and serum deprivation-induced apoptosis by activation of PI3K/Akt. Apoptosis. 2010;15:463-73 pubmed 出版商
Engesaeter B, Bonsted A, Lillehammer T, Engebraaten O, Berg K, Maelandsmo G. Photochemically mediated delivery of AdhCMV-TRAIL augments the TRAIL-induced apoptosis in colorectal cancer cell lines. Cancer Biol Ther. 2006;5:1511-20 pubmed
产品信息
SKU号 :
AF-605-NA
供货商号码 :
333
类别 :
一抗
单位尺寸 :
100微克
产品名称 :
人类/小鼠胱氨酸-天冬氨酸蛋白酶3抗体
别名 :
Apopain, apoptosis-related cysteine protease, CASP3, CASP-3, caspase 3, apoptosis-related cysteine peptidase, caspase-3, Caspase3, CPP32, CPP-32, CPP32B, CPP32SREBP cleavage activity 1, Cysteine protease CPP32, EC 3.4.22, EC 3.4.22.56, LICE-1, PARP cleavage protease, procaspase3, Protein Yama, SCA-1, Yama
缓冲液 :
Lyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
克隆性 :
多克隆
浓度 :
LYOPH
共轭标签 :
未共轭
dilution :
Western Blot 0.5 ug/mL, Simple Western 5 ug/mL, Immunoprecipitation 1 ug/10^6 cells
宿主 :
山羊
免疫原 :
E. coli -derived recombinant human Caspase-3, Met1-His277, Accession # AAA65015
抗体亚型 :
IgG
防腐剂 :
无防腐剂
纯度 :
免疫原亲和纯化
物种 :
人类, 小鼠
特异性 :
Detects human Caspase-3.
储存 :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
用途 :
In Simple Western only 10-15 uL of the recommended dilution is used per data point.
基因符号 :
CASP3
species abr :
Hu, Mu
reconstitution instructions :
Reconstitute at 0.2 mg/mL in sterile PBS.
disabled countries :
例如, KP, KR, MX, MY, TR, TW, VN
catalog number base :
AF-605-NA
display host clonality :
山羊多克隆
状态 :
活性的
Appended Catalog Number :
AF-605-NA
品牌 :
R&D Systems
top caption :
Detection of Human and Mouse Caspase-3 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line and DA3 mouse myeloma cell line untreated (-) or treated (+) with 1 µg/mL Staurosporine (STS) for 12 hours. PVDF Membrane was probed with 0.5 µg/mL of Goat Anti-Human Caspase-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-605-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017 ). Specific bands were detected for Caspase-3 precursor at approximately 34 kDa (as indicated) and Caspase-3 (cleaved) at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2 .
靶标 :
胱氨酸-天冬氨酸蛋白酶3
accessionNumbers :
AAA65015
应用 :
Western Blot, Simple Western, Immunoprecipitation
产品关键词 :
AF-605, h/mCaspase-3 Aff Pur PAb, Goat Anti-Human Caspase-3 Antigen Affinity-purified Polyclonal Antibody, Anti-Caspase-3, Goat Anti Human/Mouse Caspase-3 Antigen Affinity purified Polyclonal Antibody, Anti-hCaspase-3, Anti-mCaspase-3, SC-7148, sc-7272
美元 :
369美元
GBP :
280
欧元 :
360
CAN :
406
SEK :
3647
NOK :
3408
CHF :
595
DKK :
2826
applications abr :
WB, Simple Western, IP
default size :
TRUE
top image title :
P align=left Detection of Human and Mouse Caspase-3 by Western Blot.
toxin :
FALSE
controlled canada :
FALSE
controlled uk :
FALSE
controlled usa :
FALSE
nfs usa :
FALSE
铅时间天 :
3
PubMedIds :
16785446, 17012835, 18319333, 19997978, 27076518
铅时间描述 :
1-3 Business Days
CZK :
9720
HUF :
113040
更多信息或购买 :
安迪生物R&D 产品网页
公司信息
安迪生物R&D
上海市长宁区延安西路726号华敏翰尊国际大厦24A1
info@rndsystemschina.com.cn
www.rndsystemschina.com.cn
21 52380373
公司总部: 美国