产品简要
信息索取 :
公司名称 :
安迪生物R&D
产品类型 :
抗体
产品名称 :
Human/Mouse/Rat Phospho-Akt (S473) Pan Specific Antibody
目录 :
AF887
规格 :
100微克
价格 :
289美元
克隆性 :
多克隆
宿主 :
兔
共轭标签 :
nonconjugated
抗原修饰 :
磷酸化
反应物种 :
人类, 小鼠, 大鼠
应用 :
免疫印迹, 免疫组化
更多信息或购买 :
安迪生物R&D 产品网页
文章摘录数: 26
已发表的文献中的应用方法／种属／稀释度参考文献
  • 免疫印迹; 小鼠; 1:400
Dicay M, Hirota C, Ronaghan N, Peplowski M, Zaheer R, Carati C, et al. Interferon-γ suppresses intestinal epithelial aquaporin-1 expression via Janus kinase and STAT3 activation. PLoS ONE. 2015;10:e0118713 pubmed 出版商
  • 免疫印迹; 人类; 1:4000
Dudakovic A, Camilleri E, Lewallen E, McGee-Lawrence M, Riester S, Kakar S, et al. Histone deacetylase inhibition destabilizes the multi-potent state of uncommitted adipose-derived mesenchymal stromal cells. J Cell Physiol. 2015;230:52-62 pubmed 出版商
  • 免疫印迹; 人类
von Roemeling C, Radisky D, Marlow L, Cooper S, Grebe S, ANASTASIADIS P, et al. Neuronal pentraxin 2 supports clear cell renal cell carcinoma by activating the AMPA-selective glutamate receptor-4. Cancer Res. 2014;74:4796-810 pubmed 出版商
  • 免疫印迹; 人类; 1:2000
Wu G, Dickerson E. Frequent and increased expression of human METCAM/MUC18 in cancer tissues and metastatic lesions is associated with the clinical progression of human ovarian carcinoma. Taiwan J Obstet Gynecol. 2014;53:509-17 pubmed 出版商
  • 免疫印迹; 人类; 1:50000
Hamakawa T, Sasaki S, Shibata Y, Imura M, Kubota Y, Kojima Y, et al. Interleukin-18 may lead to benign prostatic hyperplasia via thrombospondin-1 production in prostatic smooth muscle cells. Prostate. 2014;74:590-601 pubmed 出版商
  • 免疫印迹; 小鼠; 1:500
Brun C, Périé L, Baraige F, Vernus B, Bonnieu A, Blanquet V. Absence of hyperplasia in Gasp-1 overexpressing mice is dependent on myostatin up-regulation. Cell Physiol Biochem. 2014;34:1241-59 pubmed 出版商
Budai M, Tőzsér J, Benko S. Different dynamics of NLRP3 inflammasome-mediated IL‑1β production in GM-CSF- and M-CSF-differentiated human macrophages.. J Leukoc Biol. 2017;0: pubmed 出版商
Muhamed I, Wu J, Sehgal P, Kong X, Tajik A, Wang N, et al. E-cadherin-mediated force transduction signals regulate global cell mechanics. J Cell Sci. 2016;129:1843-54 pubmed 出版商
Werling D, Reglodi D, Banks W, Salameh T, Kovacs K, Kvarik T, et al. Ocular Delivery of PACAP1-27 Protects the Retina From Ischemic Damage in Rodents. Invest Ophthalmol Vis Sci. 2016;57:6683-6691 pubmed 出版商
Divakar S, Ramana Reddy M, Cosenza S, Baker S, Perumal D, Antonelli A, et al. Dual inhibition of CDK4/Rb and PI3K/AKT/mTOR pathways by ON123300 induces synthetic lethality in mantle cell lymphomas. Leukemia. 2016;30:86-93 pubmed 出版商
Thomsen J, Hjortebjerg R, Espelund U, Ørtoft G, Vestergaard P, Magnusson N, et al. PAPP-A proteolytic activity enhances IGF bioactivity in ascites from women with ovarian carcinoma. Oncotarget. 2015;6:32266-78 pubmed 出版商
He J, de la Monte S, Wands J. The p85beta regulatory subunit of PI3K serves as a substrate for PTEN protein phosphatase activity during insulin mediated signaling. Biochem Biophys Res Commun. 2010;397:513-9 pubmed 出版商
Buqué A, Aresti U, Calvo B, Sh Muhialdin J, Munoz A, Carrera S, et al. Thymidylate synthase expression determines pemetrexed targets and resistance development in tumour cells. PLoS ONE. 2013;8:e63338 pubmed 出版商
Mao Y, Xu J, Li Z, Zhang N, Yin H, Liu Z. The role of nuclear β-catenin accumulation in the Twist2-induced ovarian cancer EMT. PLoS ONE. 2013;8:e78200 pubmed 出版商
Deng J, Han Y, Yan C, Tian X, Tao J, Kang J, et al. Overexpressing cellular repressor of E1A-stimulated genes protects mesenchymal stem cells against hypoxia- and serum deprivation-induced apoptosis by activation of PI3K/Akt. Apoptosis. 2010;15:463-73 pubmed 出版商
Wang G, Haile S, Comuzzi B, Tien A, Wang J, Yong T, et al. Osteoblast-derived factors induce an expression signature that identifies prostate cancer metastasis and hormonal progression. Cancer Res. 2009;69:3433-42 pubmed 出版商
Han Y, Cui J, Tao J, Guo L, Guo P, Sun M, et al. CREG inhibits migration of human vascular smooth muscle cells by mediating IGF-II endocytosis. Exp Cell Res. 2009;315:3301-11 pubmed 出版商
Nevo I, Sagi-Assif O, Meshel T, Ben-Baruch A, Johrer K, Greil R, et al. The involvement of the fractalkine receptor in the transmigration of neuroblastoma cells through bone-marrow endothelial cells. Cancer Lett. 2009;273:127-39 pubmed 出版商
Maddigan A, Truitt L, Arsenault R, Freywald T, Allonby O, Dean J, et al. EphB receptors trigger Akt activation and suppress Fas receptor-induced apoptosis in malignant T lymphocytes. J Immunol. 2011;187:5983-94 pubmed 出版商
Han Y, Wu G, Deng J, Tao J, Guo L, Tian X, et al. Cellular repressor of E1A-stimulated genes inhibits human vascular smooth muscle cell apoptosis via blocking P38/JNK MAP kinase activation. J Mol Cell Cardiol. 2010;48:1225-35 pubmed 出版商
He J, Kang L, Wu T, Zhang J, Wang H, Gao H, et al. An elaborate regulation of Mammalian target of rapamycin activity is required for somatic cell reprogramming induced by defined transcription factors. Stem Cells Dev. 2012;21:2630-41 pubmed 出版商
Brumbaugh K, Johnson W, Liao W, Lin M, Houchins J, Cooper J, et al. Overview of the generation, validation, and application of phosphosite-specific antibodies. Methods Mol Biol. 2011;717:3-43 pubmed 出版商
Schoephoerster J, Frisch J, Grahek M, Wu C, He Y, Wang W, et al. Absorption control in immunohistochemistry using phospho-peptides immobilized on magnetic beads. Methods Mol Biol. 2011;717:291-300 pubmed 出版商
Haim K, Weitzenfeld P, Meshel T, Ben-Baruch A. Epidermal growth factor and estrogen act by independent pathways to additively promote the release of the angiogenic chemokine CXCL8 by breast tumor cells. Neoplasia. 2011;13:230-43 pubmed
Bifulco K, Longanesi-Cattani I, Franco P, Pavone V, Mugione P, Di Carluccio G, et al. Single amino acid substitutions in the chemotactic sequence of urokinase receptor modulate cell migration and invasion. PLoS ONE. 2012;7:e44806 pubmed 出版商
Wang X, Yue P, Kim Y, Fu H, Khuri F, Sun S. Enhancing mammalian target of rapamycin (mTOR)-targeted cancer therapy by preventing mTOR/raptor inhibition-initiated, mTOR/rictor-independent Akt activation. Cancer Res. 2008;68:7409-18 pubmed 出版商
产品信息
SKU号 :
AF887
供货商号码 :
333
类别 :
一抗
单位尺寸 :
100微克
产品名称 :
Human/Mouse/Rat Phospho-Akt (S473) Pan Specific Antibody
缓冲液 :
Lyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
克隆性 :
多克隆
浓度 :
LYOPH
共轭标签 :
未共轭
dilution :
Western Blot 0.5 ug/mL, Simple Western 5 ug/mL, Immunohistochemistry 5-15 ug/mL, Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells, CyTOF-ready
宿主 :
兔
免疫原 :
Phosphopeptide containing human, mouse, and rat Akt1 S473 site
抗体亚型 :
IgG
修饰 :
p Ser473
防腐剂 :
无防腐剂
纯度 :
抗原和蛋白质A亲和纯化
物种 :
人类, 小鼠, 大鼠
特异性 :
Detects human, mouse and rat Akt1, Akt2 and Akt3, when phosphorylated at S473, S474 and S472, respectively.
储存 :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
用途 :
In Simple Western only 10-15 uL of the recommended dilution is used per data point.
基因符号 :
AKT1
species abr :
Hu, Mu, Rt
reconstitution instructions :
Reconstitute at 0.2 mg/mL in sterile PBS.
disabled countries :
例如, KP, KR, MX, MY, TR, TW, VN
catalog number base :
AF887
display host clonality :
兔多克隆
状态 :
活性的
Appended Catalog Number :
AF887
品牌 :
R&D Systems
top caption :
Detection of Mouse and Human Phospho-Akt (S473) Pan Specific by Western Blot. Western blot shows lysates of NIH-3T3 mouse embryonic fibroblast cell line untreated (-) or treated (+) with 100 ng/mL Human PDGF (Catalog # 120-HD ) for 15 minutes and MCF-7 human breast cancer cell line untreated or treated with 100 ng/mL Recombinant Human IGF-1 (Catalog # 291-G1 ) for 15 minutes. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-Akt (S473) Pan Specific Antigen Affinity-purified Polyclonal Antibody (Catalog # AF887), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008 ). A specific band was detected for Phospho-Akt (S473) Pan Specific at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1 .
靶标 :
AKT
应用 :
Western Blot, Simple Western, Immunohistochemistry, Intracellular Staining by Flow Cytometry, CyTOF-ready
产品关键词 :
AF887, pAKT, Phospho Akt S473 Aff Pur PAb, Rabbit Anti-Human/Mouse/Rat Phospho-Akt (S473) Pan Specific Polyclonal Antibody, Anti-Akt, Rabbit Anti Human/Mouse/Rat Phospho-Akt (S473) Pan Specific Antigen Affinity purified Polyclonal Antibody, Anti-hAkt, Anti-hAkt1, Anti-hAkt3, Anti-hAkt2, Anti-mAkt, Anti-mAkt1, Anti-mAkt3, Anti-mAkt2, Anti-rAkt, Anti-rAkt1, Anti-rAkt2, Anti-rAkt3, sc-7985-R
美元 :
289美元
GBP :
220
欧元 :
290
CAN :
318
SEK :
2891
NOK :
2702
CHF :
470
DKK :
2241
applications abr :
WB, Simple Western, IHC, ICFlow, CyTOF-ready
default size :
TRUE
top image title :
P align=left Detection of Mouse and Human Phospho-Akt (S473) Pan Specific by Western Blot.
toxin :
FALSE
controlled canada :
FALSE
controlled uk :
FALSE
controlled usa :
FALSE
nfs usa :
FALSE
铅时间天 :
3
PubMedIds :
18778890, 18794129, 19351846, 19769965, 19997978, 20060003, 22039307, 22471963, 23049759, 24244294, 25793528, 26174628, 27936470, 28232386
铅时间描述 :
1-3 Business Days
CZK :
7830
HUF :
91060
更多信息或购买 :
安迪生物R&D 产品网页
公司信息
安迪生物R&D
上海市长宁区延安西路726号华敏翰尊国际大厦24A1
info@rndsystemschina.com.cn
www.rndsystemschina.com.cn
21 52380373
公司总部: 美国