产品简要
信息索取 :
公司名称 :
安迪生物R&D
产品类型 :
抗体
产品名称 :
Human/Mouse/Rat Akt Pan Specific Antibody
目录 :
MAB2055
规格 :
100微克
价格 :
269美元
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
nonconjugated
克隆名称 :
281046
反应物种 :
人类, 小鼠, 大鼠
应用 :
免疫印迹, 免疫细胞化学
更多信息或购买 :
安迪生物R&D 产品网页
文章摘录数: 8
已发表的文献中的应用方法／种属／稀释度参考文献
  • 免疫印迹; 小鼠; 1:5000
Brun C, Périé L, Baraige F, Vernus B, Bonnieu A, Blanquet V. Absence of hyperplasia in Gasp-1 overexpressing mice is dependent on myostatin up-regulation. Cell Physiol Biochem. 2014;34:1241-59 pubmed 出版商
Liu Y, Takahashi Y, Desai N, Zhang J, Serfass J, Shi Y, et al. Bif-1 deficiency impairs lipid homeostasis and causes obesity accompanied by insulin resistance. Sci Rep. 2016;6:20453 pubmed 出版商
Milone M, Pucci B, Bifulco K, Iannelli F, Lombardi R, Ciardiello C, et al. Proteomic analysis of zoledronic-acid resistant prostate cancer cells unveils novel pathways characterizing an invasive phenotype. Oncotarget. 2015;6:5324-41 pubmed
Tolvanen T, Dash S, Polianskyte-Prause Z, Dumont V, Lehtonen S. Lack of CD2AP disrupts Glut4 trafficking and attenuates glucose uptake in podocytes. J Cell Sci. 2015;128:4588-600 pubmed 出版商
Han Y, Wu G, Deng J, Tao J, Guo L, Tian X, et al. Cellular repressor of E1A-stimulated genes inhibits human vascular smooth muscle cell apoptosis via blocking P38/JNK MAP kinase activation. J Mol Cell Cardiol. 2010;48:1225-35 pubmed 出版商
Salmeen A, Park B, Meyer T. The NADPH oxidases NOX4 and DUOX2 regulate cell cycle entry via a p53-dependent pathway. Oncogene. 2010;29:4473-84 pubmed 出版商
Deng J, Han Y, Yan C, Tian X, Tao J, Kang J, et al. Overexpressing cellular repressor of E1A-stimulated genes protects mesenchymal stem cells against hypoxia- and serum deprivation-induced apoptosis by activation of PI3K/Akt. Apoptosis. 2010;15:463-73 pubmed 出版商
Bifulco K, Longanesi-Cattani I, Franco P, Pavone V, Mugione P, Di Carluccio G, et al. Single amino acid substitutions in the chemotactic sequence of urokinase receptor modulate cell migration and invasion. PLoS ONE. 2012;7:e44806 pubmed 出版商
产品信息
SKU号 :
MAB2055
供货商号码 :
333
类别 :
一抗
单位尺寸 :
100微克
产品名称 :
Human/Mouse/Rat Akt Pan Specific Antibody
缓冲液 :
Lyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
克隆性 :
单克隆
克隆 :
281046
浓度 :
LYOPH
共轭标签 :
未共轭
dilution :
Western Blot 0.2 ug/mL, Simple Western 2 ug/mL, Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells, Immunocytochemistry 8-25 ug/mL, CyTOF-ready
宿主 :
小鼠
免疫原 :
E. coli -derived recombinant human Akt1, Ser2-Ala480, Accession # P31749
抗体亚型 :
IgG2b
防腐剂 :
无防腐剂
纯度 :
Protein A or G purified from hybridoma culture supernatant
物种 :
人类, 小鼠, 大鼠
特异性 :
Detects human, mouse and rat Akt in direct ELISAs and Western blots.
储存 :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
用途 :
In Simple Western only 10-15 uL of the recommended dilution is used per data point.
基因符号 :
AKT1
species abr :
Hu, Mu, Rt
reconstitution instructions :
Reconstitute at 0.5 mg/mL in sterile PBS.
disabled countries :
例如, KP, KR, MX, MY, TR, TW, VN
catalog number base :
MAB2055
display host clonality :
小鼠单克隆
状态 :
活性的
Appended Catalog Number :
MAB2055
品牌 :
R&D Systems
top caption :
Detection of Human/Mouse Akt by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line, MBA-MB-123 human breast cancer cell line, and TS1 mouse helper T cell line. PVDF membrane was probed with 0.2 µg/mL Mouse Anti-Human/Mouse/Rat Akt Pan Specific Monoclonal Antibody (Catalog # MAB2055) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007 ). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4 .
靶标 :
AKT
accessionNumbers :
P31749
应用 :
Western Blot, Simple Western, Intracellular Staining by Flow Cytometry, Immunocytochemistry, CyTOF-ready
产品关键词 :
MAB2055, h/m/rAkt Pan MAb Cl 281046, Mouse Anti-Human/Mouse/Rat Akt Pan Specific Monoclonal Antibody, Anti-Akt, Mouse Anti Human/Mouse/Rat Akt Pan Specific Monoclonal Antibody, Anti-hAkt, Anti-hAkt1, Anti-hAkt3, Anti-hAkt2, Anti-mAkt, Anti-mAkt1, Anti-mAkt3, Anti-mAkt2, Anti-rAkt, Anti-rAkt1, Anti-rAkt2, Anti-rAkt3, sc-8312
美元 :
269美元
GBP :
203
欧元 :
263
CAN :
296
SEK :
2702
NOK :
2525
CHF :
415
DKK :
2094
applications abr :
WB, Simple Western, ICFlow, ICC, CyTOF-ready
default size :
TRUE
top image title :
Detection of Human/Mouse Akt by Western Blot.
toxin :
FALSE
controlled canada :
FALSE
controlled uk :
FALSE
controlled usa :
FALSE
nfs usa :
FALSE
铅时间天 :
3
PubMedIds :
19997978, 20060003, 23049759, 26546360
铅时间描述 :
1-3 Business Days
CZK :
7101
HUF :
82582
更多信息或购买 :
安迪生物R&D 产品网页
公司信息
安迪生物R&D
上海市长宁区延安西路726号华敏翰尊国际大厦24A1
info@rndsystemschina.com.cn
www.rndsystemschina.com.cn
21 52380373
公司总部: 美国