产品简要
公司名称 :
Rockland Immunochemicals
产品类型 :
抗体
产品名称 :
血管生成素1抗体
目录 :
100-401-403
规格 :
200
价格 :
https://www.rockland.com/search/?searchString=100-401-403
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
反应物种 :
人类, 小鼠
应用 :
免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫沉淀
更多信息或购买 :
文章摘录数: 2
参考文献
Egginton S, Hussain A, Hall Jones J, Chaudhry B, Syeda F, Glen K. Shear stress-induced angiogenesis in mouse muscle is independent of the vasodilator mechanism and quickly reversible. Acta Physiol (Oxf). 2016;218:153-166 pubmed 出版商
Dallabrida S, Ismail N, Pravda E, Parodi E, Dickie R, Durand E, et al. Integrin binding angiopoietin-1 monomers reduce cardiac hypertrophy. FASEB J. 2008;22:3010-23 pubmed 出版商
产品信息
目录号 :
100-401-403
名称 :
抗血管生成素1(兔)抗体-100-401
显示名称 :
血管生成素1抗体
应用注释 :
Anti-Antiopoietin-1 Antiserum has been tested in ELISA, western blot, immunohistochemistry, and immunofluorescence and is suitable for other antibody based assays.  A 1:500 dilution is recommended for western blotting.  The reaction of this antiserum directly with cell supernatants may result in high background due to reactivity of components in the serum.  This can be alleviated by first immunoprecipitating the antibody:antigen complex and then detecting the antigen. This method results in a very clean and strong signal.  Both Ang-1 and Ang-2 proteins have predicted molecular weights of approximately 57 kDa and appear on western blots close to their predicted molecular weights.  In some instances additional bands may be seen at approximately 75 kDa which represent highly glycosylated forms of the protein that migrate at a higher apparent molecular weight.
缓冲液 :
0.02 M钾磷酸盐, 0.15 M氯化钠, pH值7.2
克隆性 :
多克隆
浓度值 :
80毫克/毫升
浓度定义 :
by Refractometry
共轭 :
(None)
规格 :
200
默认单元 :
微升
ELISA稀释浓度 :
1:5, 000 - 1:25, 000
免疫组化稀释度 :
1:200-1:800
免疫沉淀稀释度 :
User Optimized
免疫印迹稀释浓度 :
1:500-1:2,000
有效期 :
Expiration date is one (1) year from date of receipt.
格式 :
抗血清
基因名称 :
Angpt1
宿主动物 :
一般免责声明 :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
免疫原 :
This whole rabbit serum was prepared by repeated immunizations with a synthetic peptide corresponding to a N-terminus region near aa 15-45 of mouse angiopoietin 1 protein conjugated to KLH using maleimide. A residue of cysteine was added to the amino terminal end to facilitate coupling.
包装类型 :
干冰
物理状态 :
Liquid (sterile filtered)
防腐剂 :
0.01%(w/v)叠氮化钠
纯度和特异性 :
Anti-Angiopoietin 1 is directed against mouse angiopoietin-1 and shows no reactivity with mouse angiopoietin-2.  This reagent cross-reacts with human angiopoietin 1.  Partially cross reactivity is noted with human angiopoietin 2. This product was prepared from monospecific antiserum by a delipidation and defibrination.
物种活性 :
人类;小鼠
检测过的应用 :
酶联免疫吸附测定;IF;免疫组化;免疫印迹
储存 :
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
同义词 :
兔抗血管生成素1抗体, 兔抗ANG1抗体, AGP 1抗体, AGP1抗体, AGPT抗体, ANG 1抗体, Angiopoietin1抗体, ANGPT 1抗体, ANGPT1抗体, KIAA0003抗体
靶物种 :
小鼠
背景 :
Anti-Antiopoietin-1 Antibody, which recognizes Angiopoietin-1 (Ang-1), has importance in the development of the endothelium by regulating tyrosine phosphorylation of the membrane receptor Tie-2/Tek. Ang-1 binding to Tie-2/Tek causes phosphorylation of the receptor. Ang-2 competes for this binding, and thus blocks receptor phosphorylation. Ang-1 has a potential fibrinogen-like domain at the carboxyl terminus and coiled-coil regions in the amino terminus. Ang-1 is prominently expressed in the myocardium of atrium and ventricle, mesenchymal and smooth muscle cells surrounding most blood vessels, and lung. In the adult, Ang-1 is also expressed in the heart and liver. Anti-Antiopoietin-1 Antiserum antibody is suitable for cardiovascular research.
免疫原类型 :
共轭标签肽
低内毒素 :
No
样品尺寸 :
No
靶活性结构域 :
N末端
应用文本 :
酶联免疫吸附测定, 免疫组化, 免疫沉淀, 免疫印迹
其他 :
User Optimized
类别 :
一抗
共轭名称 :
未共轭
2024价格 :
$475.00
UniProt数据库 :
O08538
NCBI :
46048213
最初图像名称 :
Anti-Ang-1 Antibody - Western Blot
更多信息或购买 :
公司信息
Rockland Immunochemicals
321 Jones Blvd
Pottstown, PA 19464
orders@rockland-inc.com
https://www.rockland.com/
800-656-7625
公司总部: 美国
Rockland Immunochemicals, Inc.位于宾夕法尼亚州的费城西部,为基础研究、应用研究及临床研究提供磷酸化位点特异性抗体及抗体相关研究工具。公司的技术平台是由一批经验丰富的科学家建立,利用In vitro及In vivo方法制备并纯化单抗及多抗,这些抗体应用于免疫印迹、ELISA、免疫组化、荧光检测、高通量筛选及诊断试剂盒生产等。常用一抗主要应用于以下研究:Akt通路、载脂蛋白、细胞凋亡/细胞周期、细胞因子、细胞信号、酶类、胞外基质、转录因子及泛素相关领域。