抗体

NOTCH 2抗体

100-401-408

200

https://www.rockland.com/search/?searchString=100-401-408

多克隆

domestic rabbit

未共轭

免疫印迹, 酶联免疫吸附测定, 免疫组化

Anti-NOTCH 2 (Cleaved N terminal) (Human specific) (RABBIT) Antibody - 100-401-408

NOTCH 2抗体

Anti-Notch2 has been tested in ELISA, WB, and IHC. Anti-NOTCH-2 has a strong response was detected by ELISA against the immunizing peptide. This product was assayed against the peptide immunogen in a standard capture ELISA using Peroxidase conjugated anti-Rabbit IgG [H&L] (Goat) (code #611-1302) and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) (code # ABTS-100) as a substrate for 30 minutes at room temperature.  A working dilution of 1:30,000 to 1:90,000 is suggested in ELISA this product.

0.02 M钾磷酸盐, 0.15 M氯化钠, pH值7.2

多克隆

75毫克/毫升

by Refractometry

(None)

200

微升

1:20, 000 - 1:100, 000

1:500

1:400 - 1:2, 000

Expiration date is one (1) year from date of receipt.

抗血清

NOTCH2

兔

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

This whole rabbit serum was prepared by repeated immunizations with a synthetic peptide corresponding to amino acid residues of human Notch 2 located near the N-terminal sequence of the cleaved N intracellular domain (NICD).

干冰

Liquid (sterile filtered)

0.01%(w/v)叠氮化钠

This antiserum is directed against human NOTCH2.  The immunogen epitope is only exposed after gamma secretase cleavage and is not accessible in the uncleaved form. Based on sequences, we expect this antibody to react as well with rat and mouse NOTCH 2. Only one amino acid difference (conservative) occurs in mouse and rat.

人类

酶联免疫吸附测定;免疫组化;免疫印迹

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

rabbit anti-Notch2 antibody, AGS2 antibody, hN2 antibody, Notch homolog 2 antibody, neurogenic locus notch homolog protein 2

人类

Anti-Notch 2 Antibody recognizes Notch2 that is synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase (S1 cleavage) in the trans-golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved (S2 cleavage) by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin-dependent gamma-secretase (S3 cleavage) to release the intracellular domain (NICD) from the membrane. Anti-NOTCH2 Antibody is useful for researchers interested in Notch1, Jagged1, Jagged2, and Delta1, as well as neuroscience, transcription and cancer research.

共轭标签肽

No

No

N末端

酶联免疫吸附测定, 免疫组化, 免疫印迹

User Optimized

一抗

未共轭

$475.00

Q04721

24041035

抗NOTCH 2抗体-免疫组化