产品简要
公司名称 :
Rockland Immunochemicals
产品类型 :
抗体
产品名称 :
PERK抗体
目录 :
100-401-962
规格 :
100微升
价格 :
369.00美元
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
反应物种 :
小鼠
应用 :
免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫沉淀
更多信息或购买 :
文章摘录数: 5
出版应用/物种/样本/稀释参考文献
  • 免疫印迹; 小鼠; 图 4c
Mukhopadhyay C, Triplett A, Bargar T, HECKMAN C, Wagner K, Naramura M. Casitas B-cell lymphoma (Cbl) proteins protect mammary epithelial cells from proteotoxicity of active c-Src accumulation. Proc Natl Acad Sci U S A. 2016;113:E8228-E8237 pubmed 出版商
  • 免疫印迹; 小鼠; 1:1000; 图 2e
Xu Z, Bu Y, Chitnis N, Koumenis C, Fuchs S, Diehl J. miR-216b regulation of c-Jun mediates GADD153/CHOP-dependent apoptosis. Nat Commun. 2016;7:11422 pubmed 出版商
Srinivasan M, Bhopale K, Amer S, Wan J, Kaphalia L, Ansari G, et al. Linking Dysregulated AMPK Signaling and ER Stress in Ethanol-Induced Liver Injury in Hepatic Alcohol Dehydrogenase Deficient Deer Mice. Biomolecules. 2019;9: pubmed 出版商
Maekawa T, Liu B, Nakai D, Yoshida K, Nakamura K, Yasukawa M, et al. ATF7 mediates TNF-α-induced telomere shortening. Nucleic Acids Res. 2018;46:4487-4504 pubmed 出版商
Howley B, Link L, Grelet S, El Sabban M, Howe P. A CREB3-regulated ER-Golgi trafficking signature promotes metastatic progression in breast cancer. Oncogene. 2018;37:1308-1325 pubmed 出版商
产品信息
目录号 :
100-401-962
名称 :
Anti-PKR-like Endoplasmic Reticulum Kinase (PERK) (RABBIT) Antibody - 100-401-962
显示名称 :
PERK抗体
应用注释 :
This antiserum has been tested for use in western blotting, immunoprecipitation and immunohistochemistry.  Specific conditions for reactivity should be optimized by the end user.  Expect bands approximately 150kDa by western blotting in the appropriate cell lysate or extract.
缓冲液 :
0.02 M钾磷酸盐, 0.15 M氯化钠, pH值7.2
克隆性 :
多克隆
浓度值 :
80毫克/毫升
浓度定义 :
by Refractometry
共轭 :
(None)
规格 :
100微升
默认单元 :
微升
ELISA稀释浓度 :
1:4, 000 - 1:20, 000
免疫组化稀释度 :
1:1,000
免疫荧光显微镜稀释浓度 :
User Optimized
免疫沉淀稀释度 :
10-30微升
免疫印迹稀释浓度 :
1:500 – 1:3000
有效期 :
Expiration date is one (1) year from date of opening.
格式 :
抗血清
基因名称 :
Eif2ak3
宿主动物 :
一般免责声明 :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
免疫原 :
This whole rabbit serum was prepared by repeated immunizations with a recombinant fusion protein from amino acids 601-1115 of mouse deltaN PERK.
包装类型 :
干冰
物理状态 :
Liquid (sterile filtered)
防腐剂 :
0.01%(w/v)叠氮化钠
纯度和特异性 :
This antiserum is directed against PERK and reacts with the PERK from mouse tissues. Reactivity to other species is unknown.
物种活性 :
小鼠
储存 :
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
同义词 :
rabbit anti-PKR-like Endoplasmic Reticulum Kinase Antibody, rabbit anti-PERK Antibody, PKR-like endoplasmic reticulum kinase, PERK, Eukaryotic translation initiation factor 2-alpha kinase 3
靶物种 :
小鼠
背景 :
The PKR-like endoplasmic reticulum kinase (PERK, also know as Eukaryotic translation initiation factor 2-alpha kinase 3) is a type I transmembrane protein localized to the endoplasmic reticulum (ER). PERK consists of an N-terminal ER luminal domain, a membrane-spanning region, and a cytosolic C-terminal serine/threonine kinase domain (1). The luminal domain of PERK is bound to the ER chaperone GRP78 in unstressed cells (2). PERK activation occurs upon accumulation of misfolded proteins and the ER lumen, which triggers GRP78 dissociation from PERK thereby allowing PERK dimerization and autophosphorylation (3, 4). PERK phosphorylates two established targets: the eukaryotic translation initiation factor 2 alpha (eIF2, (1)) and the Nrf2 transcription factor (5). Phosphorylation of eIF2 results in attenuation of translation initiation (6). The translational block also contributes to cell cycle arrest due to loss of the G1 regulatory protein, cyclin D1 (7). PERK-dependent phosphorylation of Nrf2 promotes transcription of phase II detoxifying enzymes which is critically important for elimination of intracellular reactive oxygen species (8). Thus, while inhibiting new protein synthesis and thereby decreasing the ER protein load PERK simultaneously induces expression of genes that help restore cellular redox homeostasis and promote survival.
免疫原类型 :
重组蛋白
低内毒素 :
No
样品尺寸 :
No
靶活性结构域 :
内部
应用文本 :
酶联免疫吸附测定, 免疫组化, IF显微镜, 免疫沉淀, 免疫印迹
其他 :
User Optimized
类别 :
一抗
共轭名称 :
未共轭
2020价格 :
369.00美元
UniProt数据库 :
Q9Z2B5
NCBI :
32451785
最初图像名称 :
抗PERK抗体-免疫印迹
更多信息或购买 :
公司信息
Rockland Immunochemicals
321 Jones Blvd
Pottstown, PA 19464
orders@rockland-inc.com
https://rockland-inc.com
800-656-7625
公司总部: 美国
Rockland Immunochemicals, Inc.位于宾夕法尼亚州的费城西部,为基础研究、应用研究及临床研究提供磷酸化位点特异性抗体及抗体相关研究工具。公司的技术平台是由一批经验丰富的科学家建立,利用In vitro及In vivo方法制备并纯化单抗及多抗,这些抗体应用于免疫印迹、ELISA、免疫组化、荧光检测、高通量筛选及诊断试剂盒生产等。常用一抗主要应用于以下研究:Akt通路、载脂蛋白、细胞凋亡/细胞周期、细胞因子、细胞信号、酶类、胞外基质、转录因子及泛素相关领域。