产品简要
公司名称 :
Rockland Immunochemicals
产品类型 :
抗体
产品名称 :
HIF-1-alpha hydroxy P564 Antibody
目录 :
100-401-A25
规格 :
100微升
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
反应物种 :
人类
应用 :
免疫印迹, 酶联免疫吸附测定, 免疫组化
更多信息或购买 :
产品信息
目录号 :
100-401-A25
名称 :
Anti-Hif-1α hydroxy P564 (RABBIT) Antibody - 100-401-A25
显示名称 :
HIF-1-alpha hydroxy P564 Antibody
应用注释 :
This antibody has been tested for use in ELISA and western blotting.  This antibody is specific for HIF-1a hydroxylated at P564.  Minimal cross-reactivity occurs with non-hydroxylated HIF-1a.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 110 kDa in size corresponding to HIF-1a hydroxyl P564 by western blotting in the appropriate cell lysate or extract.
缓冲液 :
0.02 M钾磷酸盐, 0.15 M氯化钠, pH值7.2
克隆性 :
多克隆
浓度值 :
65毫克/毫升
浓度定义 :
by Refractometry
共轭 :
(None)
规格 :
100微升
默认单元 :
微升
ELISA稀释浓度 :
1:5, 000 - 1:20, 000
免疫组化稀释度 :
User Optimized
免疫印迹稀释浓度 :
1:500 - 1:2, 000
有效期 :
Expiration date is one (1) year from date of opening.
格式 :
抗血清
基因名称 :
缺氧诱导因子1A
宿主动物 :
一般免责声明 :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
免疫原 :
This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region surrounding the P564 of human HIF-1a.
包装类型 :
干冰
物理状态 :
Liquid (sterile filtered)
防腐剂 :
0.01%(w/v)叠氮化钠
纯度和特异性 :
This antibody is directed against human HIF-1a hydroxyP564 and is specific for the hydroxylated form of the protein.  Minimal reactivity occurs with the non-hydroxylated form of the protein.  A BLAST analysis was used to suggest cross-reactivity with HIF-1a from human, monkey, mouse, rat, dog, bovine and Xenopus sources based on a 100% homology with the immunizing sequence.  Reactivity against homologues from other sources is not known.
物种活性 :
人类
储存 :
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
同义词 :
rabbit anti-Hif-1 alpha hydroxy P564 Antibody, Hypoxia-inducible factor 1-alpha antibody, ARNT-interacting protein antibody, Member of PAS protein 1 antibody, Basic-helix-loop-helix-PAS protein MOP1 antibody, PAS domain-containing protein 8 antibody, Class E basic helix-loop-helix protein 78 antibody
靶物种 :
人类
背景 :
This antibody is designed, produced, and validated as part of a collaboration between Rockland and the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Tumor hypoxia often directly correlates with aggressive phenotype, metastasis progression and resistance to chemotherapy. HIF-1 transcription factors are dramatically induced in hypoxic areas and regulate the expression of genes necessary for tumor adaptation to conditions of low oxygen. The stabilization of HIF-1a by hypoxia is critically dependent upon the hydroxylation of certain Proline residues that exist in the oxygen-dependent degradation domain of HIF-1a. HIF factors are now considered an important therapeutic target for cancer intervention. HIF-1a is useful to researchers interested in cell metabolism, cell survival, and angiogenesis.
免疫原类型 :
多肽
低内毒素 :
No
翻译后的修饰作用 :
羟化
样品尺寸 :
No
应用文本 :
酶联免疫吸附测定, 免疫组化, 免疫印迹
其他 :
User Optimized
类别 :
一抗
共轭名称 :
未共轭
UniProt数据库 :
Q16665
NCBI :
4504385
最初图像名称 :
抗HIF-1alphaHYDROXYP564抗体-免疫印迹
更多信息或购买 :
公司信息
Rockland Immunochemicals
321 Jones Blvd
Pottstown, PA 19464
orders@rockland-inc.com
https://www.rockland.com/
800-656-7625
公司总部: 美国
Rockland Immunochemicals, Inc.位于宾夕法尼亚州的费城西部,为基础研究、应用研究及临床研究提供磷酸化位点特异性抗体及抗体相关研究工具。公司的技术平台是由一批经验丰富的科学家建立,利用In vitro及In vivo方法制备并纯化单抗及多抗,这些抗体应用于免疫印迹、ELISA、免疫组化、荧光检测、高通量筛选及诊断试剂盒生产等。常用一抗主要应用于以下研究:Akt通路、载脂蛋白、细胞凋亡/细胞周期、细胞因子、细胞信号、酶类、胞外基质、转录因子及泛素相关领域。