产品简要
公司名称 :
Rockland Immunochemicals
产品类型 :
抗体
产品名称 :
NFkB p65抗体
目录 :
100-4165
规格 :
100微升
价格 :
369美元
克隆性 :
多克隆
宿主 :
共轭标签 :
未共轭
反应物种 :
人类, 小鼠, 大鼠
应用 :
免疫印迹, 酶联免疫吸附测定, 免疫组化, 染色质免疫沉淀
更多信息或购买 :
文章摘录数: 1
出版应用/物种/样本/稀释参考文献
  • 免疫印迹; 大鼠; 图 4
Zhu X, Wang K, Zhang K, Tan X, Wu Z, Sun S, et al. Tetramethylpyrazine Protects Retinal Capillary Endothelial Cells (TR-iBRB2) against IL-1β-Induced Nitrative/Oxidative Stress. Int J Mol Sci. 2015;16:21775-90 pubmed 出版商
产品信息
目录号 :
100-4165
名称 :
Anti-NFKB p65 (Rel A) (RABBIT) Antibody - 100-4165
显示名称 :
NFkB p65抗体
应用注释 :
Anti-NFkB p65 (Rel A) is suitable for the detection by immunoblot of human and mouse NFkB p65 (Rel A), immunohistochemistry, and ELISA.  This product was also tested in a gel supershift assay and ChIP and found to be reactive against all p65 (Rel A) containing human, mouse or rat NFkB complexes using 0.5 to 1.0 µl per assay.
克隆性 :
多克隆
浓度值 :
80毫克/毫升
浓度定义 :
by Refractometry
共轭 :
(None)
规格 :
100微升
默认单元 :
微升
ELISA稀释浓度 :
1:5,000
染色质免疫共沉淀稀释 :
1微升/免疫沉淀
凝胶迁移稀释 :
05微升-1.0微升
免疫组化稀释度 :
1:400
免疫印迹稀释浓度 :
1:2, 000 - 1:5, 000
有效期 :
Expiration date is one (1) year from date of opening.
格式 :
抗血清
基因名称 :
RELA
宿主动物 :
一般免责声明 :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
免疫原 :
NFkB p65 (Rel A) peptide corresponding to a region near the C-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
包装类型 :
干冰
物理状态 :
Liquid (sterile filtered)
防腐剂 :
0.01%(w/v)叠氮化钠
纯度和特异性 :
NFkB p65 (Rel A) was prepared from monospecific antiserum by delipidation and defibrination.  Anti-NFkB p65 (Rel A) may react non-specifically with other proteins.  Control peptide (code #100-4165p) will compete only with the specific reaction of antiserum with the NFkB p65 (Rel A) subunit.
物种活性 :
人类, 小鼠, 大鼠
储存 :
Store NF-kappaB antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
同义词 :
rabbit Anti-NFKB p65 antibody, rabbit Anti-Rel A antibody, NFKB, nfkb, NF-kB, NF-kappaB, NFkappaB, Nuclear factor NF-kappa-B p65 subunit
靶物种 :
人类
背景 :
Anti-NFkB p65 Antibody recognizes NFKB p65 which is a component of NFKB. NFKB was originally identified as a factor that binds to the immunoglobulin kappa light chain enhancer in B cells. It was subsequently found in non-B cells in an inactive cytoplasmic form consisting of NFkappaB bound to IkappaB. NFkappaB was originally identified as a heterodimeric DNA binding protein complex consisting of p65 (RelA) and p50 (NFKB1) subunits. Other identified subunits include p52 (NFKB2), c-Rel, and RelB. The p65, cRel, and RelB subunits are responsible for transactivation. The p50 and p52 subunits possess DNA binding activity but limited ability to transactivate. p52 has been reported to form transcriptionally active heterodimers with the NFkappaB subunit p65, similar to p50/p65 heterodimers. The heterodimers of p52/p65 and p50/p65 are regulated by physical inactivation in the cytoplasm by IkappaBalpha. IkappaBalpha-binds to the p65 subunit, preventing nuclear localization and DNA binding. Low levels of p52 and p50 homodimers can also exist in cells.
特定参考文献 :
Gregory et al. (2004) Efficient replication by herpes simplex virus type 1 involves activation of the IkappaB kinase-IkappaB-p65 pathway. J Virol. 2004 Dec;78(24):13582-90. PMID: 15564469 - WB. Poser et al. (2003) Upregulation of HMG1 Leads to Melanoma Inhibitory Activity Expression in Malignant Melanoma Cells and Contributes to Their Malignancy Phenotype. PMID: 12665595 - IP/Co-IP. Jobin et al. (1999) Curcumin blocks cytokine-mediated NF-kappa B activation and proinflammatory gene expression by inhibiting inhibitory factor I-kappa B kinase activity. PMID: 10477620. Cao et al. (2006) NF-kappaB1 (p50) homodimers differentially regulate pro- and anti-inflammatory cytokines in macrophages. J Bio Chem. 2006 Sep 8;281(36):26041-26050. Lou et al. (2007) Glutathione depletion down-regulates tumor necrosis factor alpha-induced NF-kappaB activity via IkappaB kinase-dependent and -independent mechanisms. J Bio Chem. Oct 5;282(40):29470-29481. Yoon et al. (2008) Protein kinase A-induced phosphorylation of the p65 subunit of nuclear factor-kappaB promotes Schwann cell differentiation into a myelinating phenotype. J. Neuro. Apr 2;28(14):3738-3746. Begley et al. (2008) CXCL5 promotes prostate cancer progression. Neoplasia. Mar;10(3):244-254. PMID: 18320069-WB. Begley et al. (2005) CXCL12 overexpression and secretion by aging fibroblasts enhance human prostate epithelial proliferation in vitro. PMID: 16300481 - WB. Limpert et al. (2010) Axonal Neuregulin 1 Type III Activates NF-kappa B in Schwann Cells during Myelin Formation. J Bio Chem 285 (22): 16614-16622. Cheng et al. (2003) Cyclin E and Bcl-xL cooperatively induce cell cycle progression in c-Rel-/- B cells. Oncogene. PMID: 14627988 - WB. Fan et al. (2004) Divergent C-terminal transactivation domains of Rel/NF-kappa B proteins are critical determinants of their oncogenic potential in lymphocytes. PMID: 14647412.
免疫原类型 :
多肽
低内毒素 :
No
样品尺寸 :
No
靶活性结构域 :
C末端
应用文本 :
ELISA,Chip,Gel Shift,Immunohistochemistry,Western Blot,
其他 :
User Optimized
类别 :
一抗
共轭名称 :
未共轭
2020价格 :
369美元
UniProt数据库 :
Q04206
NCBI :
223468676
最初图像名称 :
NFkB p65 (Rel A) antibody – IHC
更多信息或购买 :
公司信息
Rockland Immunochemicals
321 Jones Blvd
Pottstown, PA 19464
orders@rockland-inc.com
https://rockland-inc.com
800-656-7625
公司总部: 美国
Rockland Immunochemicals, Inc.位于宾夕法尼亚州的费城西部,为基础研究、应用研究及临床研究提供磷酸化位点特异性抗体及抗体相关研究工具。公司的技术平台是由一批经验丰富的科学家建立,利用In vitro及In vivo方法制备并纯化单抗及多抗,这些抗体应用于免疫印迹、ELISA、免疫组化、荧光检测、高通量筛选及诊断试剂盒生产等。常用一抗主要应用于以下研究:Akt通路、载脂蛋白、细胞凋亡/细胞周期、细胞因子、细胞信号、酶类、胞外基质、转录因子及泛素相关领域。