抗体

IL-10抗体

109-401-312-0100

100微升

多克隆

domestic rabbit

未共轭

免疫印迹, 酶联免疫吸附测定

抗人类IL-10(兔)抗体-109-401

IL-10抗体

IL-10 antiserum has been tested for use in ELISA and immunoblotting. Although not tested, this antibody may be useful in radioimmunoassay, neutralizations, immunoprecipitation, immunohistochemistry. Reactivity in other immunoassays is unknown. In Western blot analysis of natural cell products or human body fluids, multiple bands of IL-10 will appear due to the variable amount of glycosylation on the molecule. The antiserum is also useful for neutralization of human of IL-10 activity in bioassays. For neutralization, incubate the sample with a 1:400 dilution of the antiserum for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG (heat inactivated) is recommended.

多克隆

85毫克/毫升

by Refractometry

(None)

100微升

微升

1:20, 000 - 1:100, 000

1:2, 000 - 1:10, 000

Expiration date is one (1) year from date of opening.

抗血清

白细胞介素10

兔

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

The whole rabbit serum was prepared by repeated immunizations with human IL-10.

干冰

Liquid (sterile filtered)

0.01%(w/v)叠氮化钠

The antiserum detects recombinant and native IL-10 present in body fluids and cell supernatants in various assays (ie. IL-1 stimulated IL-10 production from fibroblasts). The antibody recognizes hu IL-10. The antibody will not neutralize the biological activity of murine IL-10.

人类

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

CSIF抗体, 细胞因子合成抑制因子抗体, 白细胞介素10抗体, IL10A抗体, 兔抗白细胞介素10抗体, 兔抗IL-10, Interleukin10抗体, MGC126450抗体, MGC126451抗体, TGIF抗体

人类

Anti-IL-10 Antibody recognizes IL-10 (IL-10 or IL10), also known as human cytokine synthesis inhibitory factor (CSIF), that is an anti-inflammatory cytokine. In humans IL-10 is encoded by the IL10 gene. IL-10 is produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II antigens, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. IL-10 can block NF-kB activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract and indeed patients with Crohn's disease react favorably towards treatment with bacteria producing recombinant interleukin 10, showing the importance of interleukin 10 for counteracting excessive immunity in the human body. Anti-IL-10 cytokine antibody is ideal for investigators involved Immunology and Signal Transduction research.

天然蛋白

No

No

酶联免疫吸附测定, 免疫印迹

User Optimized

一抗

未共轭

P22301

P22301.1

兔抗IL-10免疫印迹