首页
>
Rockland Immunochemicals
> Rockland Immunochemicals Akt1 抗体
AKT磷酸化S473抗体
Rockland Immunochemicals
目录: 200-301-268
小鼠 单克隆 (17F6.B11)
反应物种:
人类
,
小鼠
,
大鼠
应用:
免疫印迹
,
酶联免疫吸附测定
,
免疫组化
,
免疫沉淀
,
流式细胞仪
文章摘录数: 3
Immunohistochemistry of Mouse anti-AKT pS473 antibody. Tissue: human prostate tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: AKT pS473 antibody at 20 µg/mL for 1 h at RT. Secondary antibody: Dako's Techmate streptavidin-biotin reagents at 1:10,000 for 45 min at RT. Localization: AKT pS473 is nuclear and occasionally cytoplasmic. Staining: AKT pS473 as precipitated red signal with hematoxylin purple nuclear counterstain.
规格: 100微克
价格:
至产商
AKT抗体
Rockland Immunochemicals
目录: 100-401-401
domestic rabbit 多克隆
反应物种:
人类
,
小鼠
,
大鼠
,
鸡
应用:
免疫印迹
,
酶联免疫吸附测定
,
免疫组化
,
流式细胞仪
文章摘录数: 1
AKT Metabolic Pathway
规格: 200
价格: https://www.rockland.com/search/?searchString=100-401-401
至产商
AKT磷酸化S473抗体
Rockland Immunochemicals
目录: 600-401-268
domestic rabbit 多克隆
反应物种:
人类
,
小鼠
,
大鼠
应用:
免疫印迹
,
酶联免疫吸附测定
,
免疫组化
文章摘录数: 1
Immunohistochemistry of Rabbit Anti-Akt pS473 antibody. Tissue: human breast carcinoma. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Akt pS473 antibody at 100 dilution for 1 h at RT. Secondary antibody: Dako's Techmate streptavidin-biotin reagents at 1:10,000 for 45 min at RT. Localization: Akt pS473 is nuclear and occasionally cytoplasmic.
规格: 100微克
价格:
至产商
AKT抗体
Rockland Immunochemicals
目录: 200-301-401
小鼠 单克隆 (14E5.16C8.25F6)
反应物种:
人类
,
小鼠
,
大鼠
,
黑猩猩
应用:
免疫印迹
,
酶联免疫吸附测定
,
免疫组化
,
流式细胞仪
文章摘录数: 1
Western Blot of Mouse anti-AKT antibody. Lane 1: unstimulated NIH/3T3 cell lysates. Lane 2: PDGF stimulated NIH/3T3 cell lysates. Load: 10 µg per lane. Primary antibody: AKT antibody at 1:400 for overnight at 4°C. Secondary antibody: HRP conjugated Gt-a-Mouse IgG (p/n 610-103-121) was used at a 1:40,000 dilution for 1 h at 4° C with FemtoMax™ enhanced chemiluminescent reagent (p/n FEMTOMAX-100). Block: 5% BLOTTO (p/n B501-0500 in TBS for 2h at RT. Observed size: ~56 kDa for AKT. Other band(s): none.
Western Blot of Mouse anti-AKT antibody. Lane 1: unstimulated NIH/3T3 cell lysates. Lane 2: PDGF stimulated NIH/3T3 cell lysates. Load: 10 µg per lane. Primary antibody: AKT antibody at 1:400 for overnight at 4°C. Secondary antibody: HRP conjugated Gt-a-Mouse IgG (p/n 610-103-121) was used at a 1:40,000 dilution for 1 h at 4° C with FemtoMax™ enhanced chemiluminescent reagent (p/n FEMTOMAX-100). Block: 5% BLOTTO (p/n B501-0500 in TBS for 2h at RT. Observed size: ~56 kDa for AKT. Other band(s): none.
规格: 100微克
价格:
至产商
AKT磷酸化T308抗体
Rockland Immunochemicals
目录: 600-401-269
domestic rabbit 多克隆
反应物种:
人类
,
小鼠
,
大鼠
应用:
免疫印迹
,
酶联免疫吸附测定
,
免疫组化
,
流式细胞仪
Western Blot of Rabbit anti-Akt phospho T308 antibody. Lane 1: GST tagged AKT1 un-active recombinant protein. Lane 2: GST tagged AKT1 active recombinant protein. Load: 50 ng per lane. Primary antibody: Akt phospho T308 antibody at 1:1,000 for overnight at 4°C. Secondary antibody: DyLight™ 649 rabbit secondary antibody at 1:20,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Other band(s): none.
规格: 100微克
价格:
至产商
AKT磷酸化S473抗体
Rockland Immunochemicals
目录: 200-301-B19
小鼠 单克隆 (17F6.B11)
反应物种:
人类
,
小鼠
应用:
免疫印迹
,
酶联免疫吸附测定
,
免疫组化
,
流式细胞仪
Immunofluorescence Microscopy of Mouse Anti-AKTpS473 antibody using STED nanoscopy to evaluate AKT activation and migration. Tissue: A431 cells. Antigen retrieval: Panel A: serum starved,unstimulated cells. Panel B: serum starved, EGF stimulated for 15 mins. A massive increase in AKT-pS473 activation, as measured by intensity signal, peaked at 15 minutes and was associated with depolymerized tubulin. Staining: Panel A shows STED data (AKT-pS473, red channel) collected simultaneously with confocal signal (a-tubulin, green channel). Upon stimulation of cells with EGF, a rapid activation of AKT is observed (Panel B) along with a coincident change in the tubulin organization (yellow signal), as well as an extensive cell shape-change (cell membrane folding) and accumulation of AKTpS473 at the cell periphery.
规格: 1毫克
价格:
至产商
AKT磷酸化T308抗体
Rockland Immunochemicals
目录: 200-301-269
小鼠 单克隆 (18F3.H11)
反应物种:
人类
,
小鼠
应用:
免疫印迹
,
酶联免疫吸附测定
,
免疫组化
,
免疫沉淀
,
流式细胞仪
Immunohistochemistry of Mouse anti-AKT pT308 antibody. Tissue: human brain cerebellum tissue (40X). Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: AKT pT308antibody at 20 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: staining of Purkinje neurons and cell processes in the cerebellum, cytosolic as well as occasionally nuclear. Staining: AKT pT308 as precipitated red signal with hematoxylin purple nuclear counterstain.
规格: 100微克
价格:
至产商
AKT磷酸化S473 DyLight系列染料488共轭标签抗体
Rockland Immunochemicals
目录: 200-341-268
小鼠 单克隆 (17F6.B11)
反应物种:
人类
,
小鼠
,
大鼠
共轭标签: DyLight488
应用:
免疫印迹
,
酶联免疫吸附测定
,
免疫组化
,
流式细胞仪
Properties of DyLight™ Fluorescent Dyes.
规格: 100微克
价格:
至产商
AKT磷酸化T308 DyLight系列染料488共轭标签抗体
Rockland Immunochemicals
目录: 200-341-269
小鼠 单克隆 (18F3.H11)
反应物种:
人类
,
小鼠
,
大鼠
共轭标签: DyLight488
应用:
免疫印迹
,
酶联免疫吸附测定
,
流式细胞仪
DyLight™ dye 488#DyLight™ dyes can be used for multi-color immunofluorescence microscopy with uniform fluorescence intensity throughout the image. DyLight™ dyes are exceptionally bright and photostable and are optimized for microscopy and microarray detection methods. This image shows anti-histone detection using a DyLight™ 488 conjugate (green). Anti-Tubulin was detected using a DyLight™ 549 conjugate (red). Nuclei were counter-stained using DAPI (blue). The image was captured using an Axio Imager.Z1 (Zeiss Micro Imaging Inc).
规格: 100微克
价格:
至产商
AKT磷酸化S473 DyLight系列染料549共轭标签抗体
Rockland Immunochemicals
目录: 200-342-268
小鼠 单克隆 (17F6.B11)
反应物种:
人类
,
小鼠
,
大鼠
共轭标签: DyLight549
应用:
免疫印迹
,
酶联免疫吸附测定
,
流式细胞仪
DyLight™ dyes can be used for two-color western blot detection with low background and high signal. Anti-tubulin was detected using a DyLight™ 549 conjugate. Anti-TNFa was detected using a DyLight™ 649 conjugate. The image was captured using the Typhoon™ 9410 Imaging System.
规格: 100微克
价格:
至产商
AKT磷酸化T308 DyLight系列染料549共轭标签抗体
Rockland Immunochemicals
目录: 200-342-269
小鼠 单克隆 (18F3.H11)
反应物种:
人类
,
小鼠
,
大鼠
共轭标签: DyLight549
应用:
免疫印迹
,
酶联免疫吸附测定
,
免疫组化
,
流式细胞仪
DyLight™ dyes can be used for two-color western blot detection with low background and high signal. Anti-tubulin was detected using a DyLight™ 549 conjugate. Anti-TNFa was detected using a DyLight™ 649 conjugate. The image was captured using the Typhoon™ 9410 Imaging System.
规格: 100微克
价格:
至产商
AKT磷酸化S473 DyLight系列染料649共轭标签
Rockland Immunochemicals
目录: 200-343-268
小鼠 单克隆 (17F6.B11)
反应物种:
人类
,
小鼠
,
大鼠
共轭标签: DyLight649
应用:
免疫印迹
,
酶联免疫吸附测定
,
流式细胞仪
DyLight dyes can be used for two-color Western Blot detection with low background and high signal. Anti-tubulin was detected using a DyLight™ 549 conjugate. Anti-TNFa was detected using a DyLight™ 649 conjugate. The image was captured using the Typhoon™ 9410 Imaging System.
规格: 100微克
价格:
至产商
AKT磷酸化T308 DyLight系列染料649共轭标签抗体
Rockland Immunochemicals
目录: 200-343-269
小鼠 单克隆 (18F3.H11)
反应物种:
人类
,
小鼠
,
大鼠
共轭标签: DyLight649
应用:
免疫印迹
,
酶联免疫吸附测定
,
流式细胞仪
DyLight™ dyes can be used for two-color Western Blot detection with low background and high signal. Anti-tubulin was detected using a DyLight™ 549 conjugate. Anti-TNFa was detected using a DyLight™ 649 conjugate. The image was captured using the Typhoon™ 9410 Imaging System.
规格: 100微克
价格:
至产商
AKT磷酸化T308抗体
Rockland Immunochemicals
目录: 200-301-B25
小鼠 单克隆 (18F3.H11)
反应物种:
人类
,
小鼠
应用:
免疫印迹
,
酶联免疫吸附测定
,
免疫组化
,
流式细胞仪
Western Blot of Mouse anti-AKT pT308 antibody. Lane 1: non-phosphorylated AKT in untreated cells . Lane 2: phosphorylated AKT on PDGF stimulated NIH/3T3 cell lysates . Load: 15 µg per lane. Primary antibody: AKT pT308 antibody at a 1:4,000 dilution in TBS with 3% BSA, for 3 h at 4° C. Secondary antibody: peroxidase conjugated Gt-a-Mouse IgG (Fc) (p/n 610-1303) was used at a 1:40,000 dilution for 1 h at 4° C. Block: 3% BSA (p/n BSA-30) in TBS for 30 min at RT. Predicted/Observed size: (indicated by arrowhead at ~56 kDa). Other band(s): unspecific.
规格: 1毫克
价格:
至产商
AKT抗体
Rockland Immunochemicals
目录: 200-301-B26
小鼠 单克隆 (18F10.E5)
反应物种:
人类
,
小鼠
应用:
免疫印迹
,
酶联免疫吸附测定
,
免疫组化
,
流式细胞仪
Western Blot of Mouse anti-AKT antibody. Lane 1: unstimulated NIH/3T3 cell lysates. Lane 2: PDGF stimulated NIH/3T3 cell lysates. Load: 10 µg per lane. Primary antibody: AKT antibody at 1:400 for overnight at 4°C. Secondary antibody: HRP conjugated Gt-a-Mouse IgG (p/n 610-103-121) was used at a 1:40,000 dilution for 1 h at 4° C with FemtoMax™ enhanced chemiluminescent reagent (p/n FEMTOMAX-100). Block: 5% BLOTTO (p/n B501-0500 in TBS for 2h at RT. Observed size: ~56 kDa for AKT. Other band(s): none.
规格: 1毫克
价格:
至产商
AKT磷酸化S473抗体生物素共轭标签
Rockland Immunochemicals
目录: 200-306-268
小鼠 单克隆 (17F6.B11)
反应物种:
人类
,
小鼠
,
大鼠
共轭标签: 生物素
应用:
免疫印迹
,
酶联免疫吸附测定
,
免疫组化
,
免疫沉淀
,
流式细胞仪
Immunohistochemistry of mouse anti AKT phospho S473 biotin conjugated at 40X (left) with negative control (right) Tissue: prostate Fixation: FFPE buffered formalin 10% conc Antigen retrieval: Heat, Citrate pH 6.2. Pressure Cooker Primary antibody: 20 µg/mL for 1 h at RT Secondary antibody: Streptavidin Conj. HRP 10 ug/ml Localization: nuclear and occasionally cytoplasmic Staining: antibody as precipitated red signal with a hematoxylin purple nuclear counterstain.
规格: 50微克
价格:
至产商
AKT磷酸化T308抗体生物素共轭标签
Rockland Immunochemicals
目录: 200-306-269
小鼠 单克隆 (18F3.H11)
反应物种:
人类
,
小鼠
,
大鼠
共轭标签: 生物素
应用:
免疫印迹
,
酶联免疫吸附测定
,
免疫组化
,
免疫沉淀
,
流式细胞仪
Western Blot of Mouse anti-Akt phospho T308 Biotin Conjugated antibody. Lane 1: GST tagged AKT1 active recombinant protein. Lane 2: GST tagged AKT1 un-active recombinant protein. Load: 25 ng per lane. Primary antibody: Akt phospho T308 Biotin Conjugated antibody at 1:1,000 for overnight at 4°C. Secondary antibody: HRP Streptavidin secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 79 kDa, 79 kDa for Akt phospho T308. Other band(s): none
规格: 50微克
价格:
至产商
AKT磷酸化S473 ATTO594共轭标签抗体
Rockland Immunochemicals
目录: 200-355-268
小鼠 单克隆 (17F6.B11)
反应物种:
人类
,
小鼠
,
大鼠
共轭标签: ATTO 594
应用:
免疫印迹
,
酶联免疫吸附测定
,
流式细胞仪
ATTO dyes can be used for multicolor immunofluorescent detection with low background and high signal. Examples shown are: A. Tubulin in PtK2- male Rat Kangaroo Kidney Epithelial Cells was detected using ATTO 532 labeled secondary antibody. B. Muscle alpha-actin was stained with a mouse primary antibody and ATTO 488 anti-mouse IgG (green) while Cytokeratin was stained with polyclonal rabbit anti-cytokeratin and ATTO 647N anti-rabbit IgG (red). C. HUVEC (Human umbilical vein endothelial cells were stained with anti- Vimentin-ATTO 532 (green), anti-E-Cadherin-ATTO 655 (red) and DAPI (blue). D. Rat colon sections were stained with Anti-Aquaporin 3-ATTO 594 antibody. Hoechst 33342 (blue) is used as counterstain.
Western Blot of Mouse anti-AKT pS473 antibody Atto 594 Conjugated. Lane 1: GST Tagged AKT 1 Active Recombinant Protein. Lane 2: None. Load: 25 ng per lane. Primary antibody: None. Secondary antibody: Atto 594 mouse secondary antibody at 1:1,000 for 60 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: ~100 kDa, ~100 kDa for AKT pS473. Other band(s): None
规格: 100微克
价格:
至产商
AKT1异硫氰酸荧光素抗体
Rockland Immunochemicals
目录: 200-302-I51
小鼠 单克隆 (14E5.A2.B2.H9)
反应物种:
人类
,
小鼠
,
大鼠
共轭标签: 异硫氰酸荧光素
应用:
免疫印迹
,
酶联免疫吸附测定
,
免疫组化
,
流式细胞仪
规格: 50微克
价格:
至产商
AKT1异硫氰酸荧光素抗体
Rockland Immunochemicals
目录: 200-302-J34
小鼠 单克隆 (5E5.F5.D7)
反应物种:
人类
,
小鼠
,
大鼠
共轭标签: 异硫氰酸荧光素
应用:
免疫印迹
,
酶联免疫吸附测定
,
免疫组化
,
流式细胞仪
规格: 50微克
价格:
至产商
AKT1生物素抗体
Rockland Immunochemicals
目录: 200-306-I51
小鼠 单克隆 (14E5.A2.B2.H9)
反应物种:
人类
,
小鼠
,
大鼠
共轭标签: 生物素
应用:
免疫印迹
,
酶联免疫吸附测定
,
免疫组化
,
流式细胞仪
规格: 50微克
价格:
至产商
AKT1生物素抗体
Rockland Immunochemicals
目录: 200-306-J34
小鼠 单克隆 (5E5.F5.D7)
反应物种:
人类
,
小鼠
,
大鼠
共轭标签: 生物素
应用:
免疫印迹
,
酶联免疫吸附测定
,
免疫组化
,
流式细胞仪
规格: 50微克
价格:
至产商
AKT1 PE抗体
Rockland Immunochemicals
目录: 200-308-I51
小鼠 单克隆 (14E5.A2.B2.H9)
反应物种:
人类
,
小鼠
,
大鼠
共轭标签: RPE
应用:
免疫印迹
,
酶联免疫吸附测定
,
免疫组化
,
流式细胞仪
规格: 50微克
价格:
至产商
AKT1 PE抗体
Rockland Immunochemicals
目录: 200-308-J34
小鼠 单克隆 (5E5.F5.D7)
反应物种:
人类
,
小鼠
,
大鼠
共轭标签: RPE
应用:
免疫印迹
,
酶联免疫吸附测定
,
免疫组化
,
流式细胞仪
规格: 50微克
价格:
至产商
AKT1 APC抗体
Rockland Immunochemicals
目录: 200-326-I51
小鼠 单克隆 (14E5.A2.B2.H9)
反应物种:
人类
,
小鼠
,
大鼠
共轭标签: APC
应用:
免疫印迹
,
酶联免疫吸附测定
,
免疫组化
,
流式细胞仪
规格: 50微克
价格:
至产商
AKT1 APC抗体
Rockland Immunochemicals
目录: 200-326-J34
小鼠 单克隆 (5E5.F5.D7)
反应物种:
人类
,
小鼠
,
大鼠
共轭标签: APC
应用:
免疫印迹
,
酶联免疫吸附测定
,
免疫组化
,
流式细胞仪
规格: 50微克
价格:
至产商