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Rockland Immunochemicals
> Rockland Immunochemicals Il17a 抗体
IL-17A抗体
Rockland Immunochemicals
目录: 212-401-B32
domestic rabbit 多克隆
反应物种:
小鼠
,
大鼠
应用:
免疫印迹
,
酶联免疫吸附测定
文章摘录数: 1
Western blot using Rockland's anti-IL-17A antibody shows detection of rat recombinant IL-17A protein (lane 1). Approximately 2 µg of recombinant protein was loaded onto the gel. Primary antibody was used at a 1:1,000 dilution. The membrane was washed and reacted with a 1:20,000 dilution of DyLight™ 649 conjugated Gt-a-Rabbit IgG (p/n 611-143-122). Molecular weight estimation was made by comparison to prestained MW markers indicated at the left. Other detection systems will yield similar results.
规格: 100微克
价格:
至产商
小鼠IL-17A抗体
Rockland Immunochemicals
目录: 210-501-B32
大鼠 单克隆 (20B4.G10.F5)
反应物种:
小鼠
应用:
免疫印迹
,
酶联免疫吸附测定
,
流式细胞仪
Rockland monoclonal anti IL17A was used to detect IL-17A and separate Mouse CD4+ Cells by flow cytometry. Mouse CD4+ T cells were isolated from freshly dissected spleen by centrifugation in T cell separation media and selected by magnetic separation. Cells were grown on plates coated with anti-CD3 antibody, and stimulated with: 5 µg/mL anti-CD28, 10 ng/mL IL-1beta, 50 ng/mL mouse IL-6, 1 ng/mL TGFbeta1 and 10 µg/mL anti-mouse IFN? over 8-10 days of culture. Cells were incubated for 15-20 minutes with Add rat anti-mouse CD4 APC at a concentration of 0.125 µg/mL, washed, fixed and permeabilized and incubated with Rockland Rat anti mouse IL-17A monoclonal Antibody (210-501-B32) or controls as shown. Cells were washed, incubated in streptavidin conjugated PE, fixed and analyzed by Flow cytometry. Shown here are results for positive and negative controls.
规格: 100微克
价格:
至产商
IL-17A抗体过氧化物酶 共轭标签
Rockland Immunochemicals
目录: 212-403-B32
domestic rabbit 多克隆
反应物种:
小鼠
,
大鼠
共轭标签: HRP
应用:
免疫印迹
,
酶联免疫吸附测定
,
免疫组化
规格: 100微克
价格:
至产商
IL-17A抗体生物素共轭标签
Rockland Immunochemicals
目录: 212-406-B32
domestic rabbit 多克隆
反应物种:
小鼠
,
大鼠
共轭标签: 生物素
应用:
免疫印迹
,
酶联免疫吸附测定
,
免疫组化
Western Blot showing detection of Rat IL-17A. 50ng of Rat IL-17A (Lane 1) was run on a 4-20% gel and transferred to 0.45 µm nitrocellulose. After blocking with 5% Blotto (p/n B501-0500) 30 min at 20°C, Anti-Rat IL-17A (RABBIT) Antibody Biotin Conjugated (p/n 212-406-B32) secondary antibody was used at 1:5000 in Blocking Buffer for Fluorescent Western Blotting (p/n MB-070). HRP Streptavidin (p/n S000-03) was used at 1:40,000 in MB-070 for 30 min at 20°C and imaged using the Bio-Rad VersaDoc® 4000 MP. Arrow indicates correct 15 kDa molecular weight position expected for Rat IL-17A.
规格: 100微克
价格:
至产商
IL-17抗体
Rockland Immunochemicals
目录: 600-401-BU7
domestic rabbit 多克隆
反应物种:
人类
,
小鼠
应用:
免疫印迹
,
酶联免疫吸附测定
规格: 100微克
价格:
至产商
IL-17抗体
Rockland Immunochemicals
目录: 600-401-BU8
domestic rabbit 多克隆
反应物种:
人类
,
小鼠
应用:
免疫印迹
,
酶联免疫吸附测定
,
免疫组化
规格: 100微克
价格:
至产商