产品简要
公司名称 :
Rockland Immunochemicals
产品类型 :
二抗
产品名称 :
Rabbit TrueBlot®: Anti-Rabbit IgG HRP
目录 :
18-8816-31
规格 :
50
价格 :
110.00美元
更多信息或购买 :
文章摘录数: 66
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产品信息
目录号 :
18-8816-31
名称 :
Rabbit TrueBlot®: Anti-Rabbit IgG HRP - 18-8816-31
显示名称 :
Rabbit TrueBlot®: Anti-Rabbit IgG HRP
应用注释 :
Rabbit IgG TrueBlot may also be used for detection in immunoblotting assays that do not employ immunoprecipitation. Rabbit IgG TrueBlot® is provided as 1000X solution. To conserve reagent, we recommend incubating the blots from minigels in sealed bags (removing as much air as possible) with minimal volume (2-3 mLs). If used conservatively at 2.5mLs/blot will yield enough reagent for 20 blots. Note that there are three key procedural considerations: 1. Protein A or G should not be used for the immunoprecipitation. Use of protein A or G beads with the rabbit TrueBlot will result in contaminating bands. For immunoprecipitation, Anti-Rat IgG beads, or Anti-Rabbit IgG beads should be used for rat or rabbit immunoprecipitating antibodies, respectively. 2. Immunoprecipitate should be completely reduced. 3. BLOTTO/Milk should be used as the blocking protein for the immunoblot. All recommended dilutions for listed applications are intended as an initial recommendation, specific conditions for each protein and antibody combination should be specifically optimized by the end user.
缓冲液 :
0.02 M钾磷酸盐, 0.15 M氯化钠, pH值7.2
克隆性 :
单克隆
克隆编号 :
eB182
浓度值 :
1.0毫克/毫升
浓度定义 :
by UV absorbance at 280 nm
共轭 :
HRP ULTRA
规格 :
50
默认单元 :
微升
免疫荧光显微镜稀释浓度 :
User Optimized
免疫印迹稀释浓度 :
1:1000
有效期 :
Expiration date is six (6) months from date of opening.
格式 :
IgG
宿主动物 :
小鼠
一般免责声明 :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
包装类型 :
Wet Ice
物理状态 :
Liquid (sterile filtered)
防腐剂 :
Proclin is added as an antimicrobial agent.
纯度和特异性 :
Rabbit TrueBlot® Antibody Peroxidase Conjugate was prepared from tissue culture supernatant by Protein G affinity chromatography. Assay by Immunoelectrophoresis resulted in a single precipitin arc against Anti-Rabbit Serum. Reactivity is observed against native Rabbit IgG by both Western blot and ELISA.
物种活性 :
比利时野兔(兔)
稳定剂 :
0.1毫克/毫升牛血清白蛋白(BSA)-不含IgG和蛋白酶, 50%(v/v)甘油
储存 :
Store at -20 °C. This product is guaranteed for 1 year upon receipt, when handled and stored as instructed.
同义词 :
Anti-Rabbit IgG HRP, TrueBlot, HRP TrueBlot ULTRA, Peroxidase TrueBlot, TrueBlot for IP/WB, TrueBlot for immunoprecipitation, TrueBlot for western blotting
靶物种 :
背景 :
Rabbit IgG TrueBlot® is a unique horseradish peroxidase conjugated Anti-Rabbit IgG monoclonal secondary antibody. Rabbit IgG TrueBlot® enables detection of immunoblotted target protein bands, without hindrance by interfering immunoprecipitating immunoglobulin heavy and light chains. It is easy to generate publication-quality IP/Western Blot data with Rabbit IgG TrueBlot®, simply substitute the conventional HRP Anti-Rabbit IgG blotting reagent with Rabbit IgG TrueBlot® and follow the prescribed protocol for sample preparation and immunoblotting. Rabbit IgG TrueBlot® is ideal for use in protocols involving immunoblotting of immunoprecipitated proteins. TrueBlot preferentially detects the non-reduced form of rabbit IgG over the reduced, SDS-denatured form of IgG. When the immunoprecipitate is fully reduced immediately prior to SDS-gel electrophoresis, reactivity of Rabbit IgG TrueBlot® with the 55 kDa heavy chains and the 23 kDa light chains of the immunoprecipitating antibody is minimized thereby eliminating interference by the heavy and light chains of the immunoprecipitating antibody in IP/Western blot applications. Applications include studies examining post-translational modification (e.g., phosphorylation or acetylation) or protein-protein interactions.
检测试剂盒类型 :
Immunoprecipitation Kit
检测试剂盒和组成 :
Component
低内毒素 :
No
样品尺寸 :
No
应用文本 :
IF显微镜, 免疫印迹
类别 :
二抗
共轭名称 :
HRP TrueBlot® ULTRA for IP/WB
2022价格 :
110.00美元
最初图像名称 :
Rabbit TrueBlot® IP / Western Blot
更多信息或购买 :
公司信息
Rockland Immunochemicals
321 Jones Blvd
Pottstown, PA 19464
orders@rockland-inc.com
https://rockland-inc.com
800-656-7625
公司总部: 美国
Rockland Immunochemicals, Inc.位于宾夕法尼亚州的费城西部,为基础研究、应用研究及临床研究提供磷酸化位点特异性抗体及抗体相关研究工具。公司的技术平台是由一批经验丰富的科学家建立,利用In vitro及In vivo方法制备并纯化单抗及多抗,这些抗体应用于免疫印迹、ELISA、免疫组化、荧光检测、高通量筛选及诊断试剂盒生产等。常用一抗主要应用于以下研究:Akt通路、载脂蛋白、细胞凋亡/细胞周期、细胞因子、细胞信号、酶类、胞外基质、转录因子及泛素相关领域。