公司名称 :
Rockland Immunochemicals
产品类型 :
产品名称 :
Rabbit TrueBlot®: Anti-Rabbit IgG HRP
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规格 :
价格 :
更多信息或购买 :
文章摘录数: 67
Lear T, Lockwood K, Larsen M, Tuncer F, Kennerdell J, Morse C, et al. Kelch-like protein 42 is a profibrotic ubiquitin E3 ligase involved in systemic sclerosis. J Biol Chem. 2020;295:4171-4180 pubmed 出版商
Stark J, Opoka A, Mallela J, Devarajan P, Ma Q, Levinsky N, et al. Juvenile OLFM4-null mice are protected from sepsis. Am J Physiol Renal Physiol. 2020;318:F809-F816 pubmed 出版商
Zhao Y, Zagani R, Park S, Yoshida N, Shah P, Reinecker H. Microbial recognition by GEF-H1 controls IKKε mediated activation of IRF5. Nat Commun. 2019;10:1349 pubmed 出版商
Takimoto A, Kokubu C, Watanabe H, Sakuma T, Yamamoto T, Kondoh G, et al. Differential transactivation of the upstream aggrecan enhancer regulated by PAX1/9 depends on SOX9-driven transactivation. Sci Rep. 2019;9:4605 pubmed 出版商
Graves Deal R, Bogatcheva G, Rehman S, Lu Y, Higginbotham J, Singh B. Broad-spectrum receptor tyrosine kinase inhibitors overcome de novo and acquired modes of resistance to EGFR-targeted therapies in colorectal cancer. Oncotarget. 2019;10:1320-1333 pubmed 出版商
Mehto S, Jena K, Nath P, Chauhan S, Kolapalli S, Das S, et al. The Crohn's Disease Risk Factor IRGM Limits NLRP3 Inflammasome Activation by Impeding Its Assembly and by Mediating Its Selective Autophagy. Mol Cell. 2019;73:429-445.e7 pubmed 出版商
Menzies S, Volkmar N, van den Boomen D, Timms R, Dickson A, Nathan J, et al. The sterol-responsive RNF145 E3 ubiquitin ligase mediates the degradation of HMG-CoA reductase together with gp78 and Hrd1. elife. 2018;7: pubmed 出版商
Pazos M, Peters K, Casanova M, Palacios P, VANNIEUWENHZE M, Breukink E, et al. Z-ring membrane anchors associate with cell wall synthases to initiate bacterial cell division. Nat Commun. 2018;9:5090 pubmed 出版商
Predescu D, Qin S, Patel M, Bardita C, Bhalli R, Predescu S. Epsin15 Homology Domains: Role in the Pathogenesis of Pulmonary Arterial Hypertension. Front Physiol. 2018;9:1393 pubmed 出版商
Cheng C, Spengler R, Keiser M, Monteys A, Rieders J, Ramachandran S, et al. The long non-coding RNA NEAT1 is elevated in polyglutamine repeat expansion diseases and protects from disease gene-dependent toxicities. Hum Mol Genet. 2018;27:4303-4314 pubmed 出版商
Perraki A, DeFalco T, Derbyshire P, Avila J, Séré D, Sklenar J, et al. Phosphocode-dependent functional dichotomy of a common co-receptor in plant signalling. Nature. 2018;561:248-252 pubmed 出版商
D Souza A, Jiang Y, Cast A, Valanejad L, Wright M, Lewis K, et al. Gankyrin Promotes Tumor-Suppressor Protein Degradation to Drive Hepatocyte Proliferation. Cell Mol Gastroenterol Hepatol. 2018;6:239-255 pubmed 出版商
Pena Philippides J, Gardiner A, Caballero Garrido E, Pan R, Zhu Y, Roitbak T. Inhibition of MicroRNA-155 Supports Endothelial Tight Junction Integrity Following Oxygen-Glucose Deprivation. J Am Heart Assoc. 2018;7: pubmed 出版商
Mitchell K, Barreyro L, Todorova T, Taylor S, Antony Debré I, Narayanagari S, et al. IL1RAP potentiates multiple oncogenic signaling pathways in AML. J Exp Med. 2018;215:1709-1727 pubmed 出版商
Sekiba K, Otsuka M, Ohno M, Kishikawa T, Yamagami M, Suzuki T, et al. DHX9 regulates production of hepatitis B virus-derived circular RNA and viral protein levels. Oncotarget. 2018;9:20953-20964 pubmed 出版商
Haque M, Han B, Wang B, Wang Y, Liu A. Development of an efficient chromatin immunoprecipitation method to investigate protein-DNA interaction in oleaginous castor bean seeds. PLoS ONE. 2018;13:e0197126 pubmed 出版商
Hartmann S, Zheng F, Kyncl M, Karch S, Voelkl K, Zott B, et al. β-Secretase BACE1 Promotes Surface Expression and Function of Kv3.4 at Hippocampal Mossy Fiber Synapses. J Neurosci. 2018;38:3480-3494 pubmed 出版商
Kumar S, Jain A, Farzam F, Jia J, Gu Y, Choi S, et al. Mechanism of Stx17 recruitment to autophagosomes via IRGM and mammalian Atg8 proteins. J Cell Biol. 2018;217:997-1013 pubmed 出版商
Moriya C, Taniguchi H, Nagatoishi S, Igarashi H, Tsumoto K, Imai K. PRDM14 directly interacts with heat shock proteins HSP90α and glucose-regulated protein 78. Cancer Sci. 2018;109:373-383 pubmed 出版商
Lu H, Shamanna R, de Freitas J, Okur M, Khadka P, Kulikowicz T, et al. Cell cycle-dependent phosphorylation regulates RECQL4 pathway choice and ubiquitination in DNA double-strand break repair. Nat Commun. 2017;8:2039 pubmed 出版商
Lehmann L, Hewitt G, Aibara S, Leitner A, Marklund E, Maslen S, et al. Mechanistic Insights into Autoinhibition of the Oncogenic Chromatin Remodeler ALC1. Mol Cell. 2017;68:847-859.e7 pubmed 出版商
Nagata M, Ishino S, Yamagami T, Simons J, Kanai T, Atomi H, et al. Possible function of the second RecJ-like protein in stalled replication fork repair by interacting with Hef. Sci Rep. 2017;7:16949 pubmed 出版商
Short S, Kondo J, Smalley Freed W, Takeda H, Dohn M, Powell A, et al. p120-Catenin is an obligate haploinsufficient tumor suppressor in intestinal neoplasia. J Clin Invest. 2017;127:4462-4476 pubmed 出版商
Torregrosa Muñumer R, Forslund J, Goffart S, Pfeiffer A, Stojkovič G, Carvalho G, et al. PrimPol is required for replication reinitiation after mtDNA damage. Proc Natl Acad Sci U S A. 2017;114:11398-11403 pubmed 出版商
Mukherjee T, Chatterjee B, Dhar A, Bais S, Chawla M, Roy P, et al. A TNF-p100 pathway subverts noncanonical NF-κB signaling in inflamed secondary lymphoid organs. EMBO J. 2017;36:3501-3516 pubmed 出版商
Nagata M, Ishino S, Yamagami T, Ogino H, Simons J, Kanai T, et al. The Cdc45/RecJ-like protein forms a complex with GINS and MCM, and is important for DNA replication in Thermococcus kodakarensis. Nucleic Acids Res. 2017;45:10693-10705 pubmed 出版商
Weng T, Koh C. POPX2 phosphatase regulates apoptosis through the TAK1-IKK-NF-κB pathway. Cell Death Dis. 2017;8:e3051 pubmed 出版商
Prevost M, Pinotsis N, Dumoux M, Hayward R, Waksman G. The Legionella effector WipB is a translocated Ser/Thr phosphatase that targets the host lysosomal nutrient sensing machinery. Sci Rep. 2017;7:9450 pubmed 出版商
Van Nostrand E, Shishkin A, Pratt G, Nguyen T, Yeo G. Variation in single-nucleotide sensitivity of eCLIP derived from reverse transcription conditions. Methods. 2017;126:29-37 pubmed 出版商
Joachim J, Razi M, Judith D, Wirth M, Calamita E, Encheva V, et al. Centriolar Satellites Control GABARAP Ubiquitination and GABARAP-Mediated Autophagy. Curr Biol. 2017;27:2123-2136.e7 pubmed 出版商
Lewis K, Valanejad L, Cast A, Wright M, Wei C, Iakova P, et al. RNA Binding Protein CUGBP1 Inhibits Liver Cancer in a Phosphorylation-Dependent Manner. Mol Cell Biol. 2017;37: pubmed 出版商
Miyoshi S, Tezuka T, Arimura S, Tomono T, Okada T, Yamanashi Y. DOK7 gene therapy enhances motor activity and life span in ALS model mice. EMBO Mol Med. 2017;9:880-889 pubmed 出版商
Hu J, Du W, Shelton S, Oldham M, DiPersio C, Klein O. An FAK-YAP-mTOR Signaling Axis Regulates Stem Cell-Based Tissue Renewal in Mice. Cell Stem Cell. 2017;21:91-106.e6 pubmed 出版商
Tagawa M, Yamamoto Y, Shimbo G, Iguchi A, Xuan X, Tomihari M, et al. Gene and protein expression of a soluble form of CTLA-4 in a healthy dog. J Vet Med Sci. 2017;79:871-875 pubmed 出版商
Zhai Z, Liu W, Kaur M, Luo Y, Domenico J, Samson J, et al. NLRP1 promotes tumor growth by enhancing inflammasome activation and suppressing apoptosis in metastatic melanoma. Oncogene. 2017;36:3820-3830 pubmed 出版商
Nakashima K, Uekita T, Yano S, Kikuchi J, Nakanishi R, Sakamoto N, et al. Novel small molecule inhibiting CDCP1-PKCδ pathway reduces tumor metastasis and proliferation. Cancer Sci. 2017;108:1049-1057 pubmed 出版商
Goralska M, Fleisher L, McGahan M. Vitreous Humor Changes Expression of Iron-Handling Proteins in Lens Epithelial Cells. Invest Ophthalmol Vis Sci. 2017;58:1187-1195 pubmed 出版商
Huang J, Ku W, Chen Y, Chang Y, Chu C. Dual mechanisms regulate the nucleocytoplasmic localization of human DDX6. Sci Rep. 2017;7:42853 pubmed 出版商
Seong H, Manoharan R, Ha H. Zinc finger protein ZPR9 functions as an activator of AMPK-related serine/threonine kinase MPK38/MELK involved in ASK1/TGF-β/p53 signaling pathways. Sci Rep. 2017;7:42502 pubmed 出版商
Mansilla A, Chaves Sanjuan A, Campillo N, Semelidou O, Martínez González L, Infantes L, et al. Interference of the complex between NCS-1 and Ric8a with phenothiazines regulates synaptic function and is an approach for fragile X syndrome. Proc Natl Acad Sci U S A. 2017;114:E999-E1008 pubmed 出版商
Rivera Serrano E, Fritch E, Scholl E, Sherry B. A Cytoplasmic RNA Virus Alters the Function of the Cell Splicing Protein SRSF2. J Virol. 2017;91: pubmed 出版商
Arima M, Cui D, Kimura T, Sonoda K, Ishibashi T, Matsuda S, et al. Basigin can be a therapeutic target to restore the retinal vascular barrier function in the mouse model of diabetic retinopathy. Sci Rep. 2016;6:38445 pubmed 出版商
Zhang Y, Li Q, Youn J, Cai H. Protein Phosphotyrosine Phosphatase 1B (PTP1B) in Calpain-dependent Feedback Regulation of Vascular Endothelial Growth Factor Receptor (VEGFR2) in Endothelial Cells: IMPLICATIONS IN VEGF-DEPENDENT ANGIOGENESIS AND DIABETIC WOUND HEALING. J Biol Chem. 2017;292:407-416 pubmed 出版商
Strikoudis A, Lazaris C, Trimarchi T, Galvao Neto A, Yang Y, Ntziachristos P, et al. Regulation of transcriptional elongation in pluripotency and cell differentiation by the PHD-finger protein Phf5a. Nat Cell Biol. 2016;18:1127-1138 pubmed 出版商
Kunii M, Ohara Imaizumi M, Takahashi N, Kobayashi M, Kawakami R, Kondoh Y, et al. Opposing roles for SNAP23 in secretion in exocrine and endocrine pancreatic cells. J Cell Biol. 2016;215:121-138 pubmed
Kishikawa T, Otsuka M, Yoshikawa T, Ohno M, Ijichi H, Koike K. Satellite RNAs promote pancreatic oncogenic processes via the dysfunction of YBX1. Nat Commun. 2016;7:13006 pubmed 出版商
Weaver R, Limzerwala J, Naylor R, Jeganathan K, Baker D, van Deursen J. BubR1 alterations that reinforce mitotic surveillance act against aneuploidy and cancer. elife. 2016;5: pubmed 出版商
Hwang J, Lee J, Pallas D. Leucine Carboxyl Methyltransferase 1 (LCMT-1) Methylates Protein Phosphatase 4 (PP4) and Protein Phosphatase 6 (PP6) and Differentially Regulates the Stable Formation of Different PP4 Holoenzymes. J Biol Chem. 2016;291:21008-21019 pubmed
Peng X, Giménez Cassina A, Petrus P, Conrad M, Rydén M, Arnér E. Thioredoxin reductase 1 suppresses adipocyte differentiation and insulin responsiveness. Sci Rep. 2016;6:28080 pubmed 出版商
Aly H, Suzuki J, Watashi K, Chayama K, Hoshino S, Hijikata M, et al. RNA Exosome Complex Regulates Stability of the Hepatitis B Virus X-mRNA Transcript in a Non-stop-mediated (NSD) RNA Quality Control Mechanism. J Biol Chem. 2016;291:15958-74 pubmed 出版商
Maisonneuve P, Caillet Saguy C, Vaney M, Bibi Zainab E, Sawyer K, Raynal B, et al. Molecular Basis of the Interaction of the Human Protein Tyrosine Phosphatase Non-receptor Type 4 (PTPN4) with the Mitogen-activated Protein Kinase p38?. J Biol Chem. 2016;291:16699-708 pubmed 出版商
Saito M, Tanaka M, Misawa E, Yao R, Nabeshima K, Yamauchi K, et al. Oral administration of Aloe vera gel powder prevents UVB-induced decrease in skin elasticity via suppression of overexpression of MMPs in hairless mice. Biosci Biotechnol Biochem. 2016;80:1416-24 pubmed 出版商
Dauber K, Perdigoto C, Valdes V, Santoriello F, Cohen I, Ezhkova E. Dissecting the Roles of Polycomb Repressive Complex 2 Subunits in the Control of Skin Development. J Invest Dermatol. 2016;136:1647-1655 pubmed 出版商
Wang F, Zieman A, Coulombe P. Skin Keratins. Methods Enzymol. 2016;568:303-50 pubmed 出版商
Gonzalez Calero L, Martin Lorenzo M, Ramos Barron A, Ruiz Criado J, Maroto A, Ortiz A, et al. Urinary Kininogen-1 and Retinol binding protein-4 respond to Acute Kidney Injury: predictors of patient prognosis?. Sci Rep. 2016;6:19667 pubmed 出版商
Schumacher N, Meyer D, Mauermann A, von der Heyde J, Wolf J, Schwarz J, et al. Shedding of Endogenous Interleukin-6 Receptor (IL-6R) Is Governed by A Disintegrin and Metalloproteinase (ADAM) Proteases while a Full-length IL-6R Isoform Localizes to Circulating Microvesicles. J Biol Chem. 2015;290:26059-71 pubmed 出版商
Conde Perez A, Gros G, Longvert C, Pedersen M, Petit V, Aktary Z, et al. A caveolin-dependent and PI3K/AKT-independent role of PTEN in β-catenin transcriptional activity. Nat Commun. 2015;6:8093 pubmed 出版商
Vuong L, Chellappa K, Dhahbi J, Deans J, Fang B, Bolotin E, et al. Differential Effects of Hepatocyte Nuclear Factor 4α Isoforms on Tumor Growth and T-Cell Factor 4/AP-1 Interactions in Human Colorectal Cancer Cells. Mol Cell Biol. 2015;35:3471-90 pubmed 出版商
Heneghan J, Vandorpe D, Shmukler B, Giovinazzo J, Giovinnazo J, Raper J, et al. BH3 domain-independent apolipoprotein L1 toxicity rescued by BCL2 prosurvival proteins. Am J Physiol Cell Physiol. 2015;309:C332-47 pubmed 出版商
Ibeawuchi S, Agbor L, Quelle F, Sigmund C. Hypertension-causing Mutations in Cullin3 Protein Impair RhoA Protein Ubiquitination and Augment the Association with Substrate Adaptors. J Biol Chem. 2015;290:19208-17 pubmed 出版商
Greenfeld H, Takasaki K, Walsh M, Ersing I, Bernhardt K, Ma Y, et al. TRAF1 Coordinates Polyubiquitin Signaling to Enhance Epstein-Barr Virus LMP1-Mediated Growth and Survival Pathway Activation. PLoS Pathog. 2015;11:e1004890 pubmed 出版商
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Liu T, Vachharajani V, Millet P, Bharadwaj M, Molina A, McCall C. Sequential actions of SIRT1-RELB-SIRT3 coordinate nuclear-mitochondrial communication during immunometabolic adaptation to acute inflammation and sepsis. J Biol Chem. 2015;290:396-408 pubmed 出版商
目录号 :
名称 :
Rabbit TrueBlot®: Anti-Rabbit IgG HRP - 18-8816-33
显示名称 :
Rabbit TrueBlot®: Anti-Rabbit IgG HRP
应用注释 :
Rabbit IgG TrueBlot may also be used for detection in immunoblotting assays that do not employ immunoprecipitation. Rabbit IgG TrueBlot® is provided as 1000X solution. To conserve reagent, we recommend incubating the blots from minigels in sealed bags (removing as much air as possible) with minimal volume (2-3 mls). If used conservatively at 2.5mls/blot will yield enough reagent for 80 blots. Note that there are three key procedural considerations: 1. Protein A or G should not be used for the immunoprecipitation. Use of protein A or G beads with the rabbit TrueBlot will result in contaminating bands. For immunoprecipitation, Anti-Rat IgG beads, or Anti-Rabbit IgG beads should be used for rat or rabbit immunoprecipitating antibodies, respectively. 2. Immunoprecipitate should be completely reduced. 3. BLOTTO/Milk should be used as the blocking protein for the immunoblot. All recommended dilutions for listed applications are intended as an initial recommendation, specific conditions for each protein and antibody combination should be specifically optimized by the end user.
缓冲液 :
0.02 M钾磷酸盐, 0.15 M氯化钠, pH值7.2
克隆性 :
克隆编号 :
浓度值 :
浓度定义 :
by UV absorbance at 280 nm
共轭 :
规格 :
默认单元 :
免疫荧光显微镜稀释浓度 :
User Optimized
免疫印迹稀释浓度 :
有效期 :
Expiration date is six (6) months from date of opening.
格式 :
宿主动物 :
一般免责声明 :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
包装类型 :
Wet Ice
物理状态 :
Liquid (sterile filtered)
防腐剂 :
Proclin is added as an antimicrobial agent.
纯度和特异性 :
Rabbit TrueBlot® Antibody Peroxidase Conjugate was prepared from tissue culture supernatant by Protein G affinity chromatography. Assay by Immunoelectrophoresis resulted in a single precipitin arc against Anti-Rabbit Serum. Reactivity is observed against native Rabbit IgG by both Western blot and ELISA.
物种活性 :
稳定剂 :
0.1毫克/毫升牛血清白蛋白(BSA)-不含IgG和蛋白酶, 50%(v/v)甘油
储存 :
Store at -20 °C. This product is guaranteed for 1 year upon receipt, when handled and stored as instructed.
同义词 :
Anti-Rabbit IgG HRP, TrueBlot, HRP TrueBlot ULTRA, Peroxidase TrueBlot, TrueBlot for IP/WB, TrueBlot for immunoprecipitation, TrueBlot for western blotting
靶物种 :
背景 :
Rabbit IgG TrueBlot® is a unique horseradish peroxidase conjugated Anti-Rabbit IgG monoclonal secondary antibody. Rabbit IgG TrueBlot® enables detection of immunoblotted target protein bands, without hindrance by interfering immunoprecipitating immunoglobulin heavy and light chains. It is easy to generate publication-quality IP/Western Blot data with Rabbit IgG TrueBlot®, simply substitute the conventional HRP Anti-Rabbit IgG blotting reagent with Rabbit IgG TrueBlot® and follow the prescribed protocol for sample preparation and immunoblotting. Rabbit IgG TrueBlot® is ideal for use in protocols involving immunoblotting of immunoprecipitated proteins. TrueBlot preferentially detects the non-reduced form of rabbit IgG over the reduced, SDS-denatured form of IgG. When the immunoprecipitate is fully reduced immediately prior to SDS-gel electrophoresis, reactivity of Rabbit IgG TrueBlot® with the 55 kDa heavy chains and the 23 kDa light chains of the immunoprecipitating antibody is minimized thereby eliminating interference by the heavy and light chains of the immunoprecipitating antibody in IP/Western blot applications. Applications include studies examining post-translational modification (e.g., phosphorylation or acetylation) or protein-protein interactions.
低内毒素 :
样品尺寸 :
应用文本 :
IF显微镜, 免疫印迹
类别 :
共轭名称 :
HRP TrueBlot® ULTRA for IP/WB
2022价格 :
最初图像名称 :
Rabbit TrueBlot® IP / Western Blot
更多信息或购买 :
Rockland Immunochemicals
321 Jones Blvd
Pottstown, PA 19464
公司总部: 美国
Rockland Immunochemicals, Inc.位于宾夕法尼亚州的费城西部,为基础研究、应用研究及临床研究提供磷酸化位点特异性抗体及抗体相关研究工具。公司的技术平台是由一批经验丰富的科学家建立,利用In vitro及In vivo方法制备并纯化单抗及多抗,这些抗体应用于免疫印迹、ELISA、免疫组化、荧光检测、高通量筛选及诊断试剂盒生产等。常用一抗主要应用于以下研究:Akt通路、载脂蛋白、细胞凋亡/细胞周期、细胞因子、细胞信号、酶类、胞外基质、转录因子及泛素相关领域。