公司名称 :
Rockland Immunochemicals
产品类型 :
产品名称 :
Mouse TrueBlot® ULTRA: Anti-Mouse Ig HRP
目录 :
规格 :
价格 :
更多信息或购买 :
文章摘录数: 57
Lear T, Lockwood K, Larsen M, Tuncer F, Kennerdell J, Morse C, et al. Kelch-like protein 42 is a profibrotic ubiquitin E3 ligase involved in systemic sclerosis. J Biol Chem. 2020;295:4171-4180 pubmed 出版商
Zhao Y, Zagani R, Park S, Yoshida N, Shah P, Reinecker H. Microbial recognition by GEF-H1 controls IKKε mediated activation of IRF5. Nat Commun. 2019;10:1349 pubmed 出版商
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Mehto S, Jena K, Nath P, Chauhan S, Kolapalli S, Das S, et al. The Crohn's Disease Risk Factor IRGM Limits NLRP3 Inflammasome Activation by Impeding Its Assembly and by Mediating Its Selective Autophagy. Mol Cell. 2019;73:429-445.e7 pubmed 出版商
Menzies S, Volkmar N, van den Boomen D, Timms R, Dickson A, Nathan J, et al. The sterol-responsive RNF145 E3 ubiquitin ligase mediates the degradation of HMG-CoA reductase together with gp78 and Hrd1. elife. 2018;7: pubmed 出版商
Jeon A, Kim J. PDI Knockdown Inhibits Seizure Activity in Acute Seizure and Chronic Epilepsy Rat Models via S-Nitrosylation-Independent Thiolation on NMDA Receptor. Front Cell Neurosci. 2018;12:438 pubmed 出版商
Ikeuchi T, de Vega S, Forcinito P, Doyle A, Amaral J, Rodriguez I, et al. Extracellular Protein Fibulin-7 and Its C-Terminal Fragment Have In Vivo Antiangiogenic Activity. Sci Rep. 2018;8:17654 pubmed 出版商
Brekk O, Moskites A, Isacson O, Hallett P. Lipid-dependent deposition of alpha-synuclein and Tau on neuronal Secretogranin II-positive vesicular membranes with age. Sci Rep. 2018;8:15207 pubmed 出版商
Kistler K, Trcek T, Hurd T, Chen R, Liang F, Sall J, et al. Phase transitioned nuclear Oskar promotes cell division of Drosophila primordial germ cells. elife. 2018;7: pubmed 出版商
D Souza A, Jiang Y, Cast A, Valanejad L, Wright M, Lewis K, et al. Gankyrin Promotes Tumor-Suppressor Protein Degradation to Drive Hepatocyte Proliferation. Cell Mol Gastroenterol Hepatol. 2018;6:239-255 pubmed 出版商
Park Y, Ahn J, Cho M, Lee J. ATP depletion during mitotic arrest induces mitotic slippage and APC/CCdh1-dependent cyclin B1 degradation. Exp Mol Med. 2018;50:46 pubmed 出版商
Guarnieri A, Towers C, Drasin D, Oliphant M, Andrysik Z, Hotz T, et al. The miR-106b-25 cluster mediates breast tumor initiation through activation of NOTCH1 via direct repression of NEDD4L. Oncogene. 2018;37:3879-3893 pubmed 出版商
Pasquin S, Chehboun S, Dejda A, Meliani Y, Savin V, Warner G, et al. Effect of human very low-density lipoproteins on cardiotrophin-like cytokine factor 1 (CLCF1) activity. Sci Rep. 2018;8:3990 pubmed 出版商
Liu X, Reitsma J, Mamrosh J, Zhang Y, Straube R, Deshaies R. Cand1-Mediated Adaptive Exchange Mechanism Enables Variation in F-Box Protein Expression. Mol Cell. 2018;69:773-786.e6 pubmed 出版商
Lu H, Shamanna R, de Freitas J, Okur M, Khadka P, Kulikowicz T, et al. Cell cycle-dependent phosphorylation regulates RECQL4 pathway choice and ubiquitination in DNA double-strand break repair. Nat Commun. 2017;8:2039 pubmed 出版商
Lehmann L, Hewitt G, Aibara S, Leitner A, Marklund E, Maslen S, et al. Mechanistic Insights into Autoinhibition of the Oncogenic Chromatin Remodeler ALC1. Mol Cell. 2017;68:847-859.e7 pubmed 出版商
Hiyama A, Takemiya A, Munemasa S, Okuma E, Sugiyama N, Tada Y, et al. Blue light and CO2 signals converge to regulate light-induced stomatal opening. Nat Commun. 2017;8:1284 pubmed 出版商
Weng T, Koh C. POPX2 phosphatase regulates apoptosis through the TAK1-IKK-NF-κB pathway. Cell Death Dis. 2017;8:e3051 pubmed 出版商
Miller C, Smith E, Cundiff J, Knodler L, Bailey Blackburn J, Lupashin V, et al. A Brucella Type IV Effector Targets the COG Tethering Complex to Remodel Host Secretory Traffic and Promote Intracellular Replication. Cell Host Microbe. 2017;22:317-329.e7 pubmed 出版商
Joachim J, Razi M, Judith D, Wirth M, Calamita E, Encheva V, et al. Centriolar Satellites Control GABARAP Ubiquitination and GABARAP-Mediated Autophagy. Curr Biol. 2017;27:2123-2136.e7 pubmed 出版商
Mori M, Hazan R, Danielian P, Mahoney J, Li H, LU J, et al. Cytoplasmic E2f4 forms organizing centres for initiation of centriole amplification during multiciliogenesis. Nat Commun. 2017;8:15857 pubmed 出版商
Bononi A, Giorgi C, Patergnani S, Larson D, Verbruggen K, Tanji M, et al. BAP1 regulates IP3R3-mediated Ca2+ flux to mitochondria suppressing cell transformation. Nature. 2017;546:549-553 pubmed 出版商
Lewis K, Valanejad L, Cast A, Wright M, Wei C, Iakova P, et al. RNA Binding Protein CUGBP1 Inhibits Liver Cancer in a Phosphorylation-Dependent Manner. Mol Cell Biol. 2017;37: pubmed 出版商
Hu J, Du W, Shelton S, Oldham M, DiPersio C, Klein O. An FAK-YAP-mTOR Signaling Axis Regulates Stem Cell-Based Tissue Renewal in Mice. Cell Stem Cell. 2017;21:91-106.e6 pubmed 出版商
Bourens M, Barrientos A. Human mitochondrial cytochrome c oxidase assembly factor COX18 acts transiently as a membrane insertase within the subunit 2 maturation module. J Biol Chem. 2017;292:7774-7783 pubmed 出版商
Kashihara T, Nakada T, Kojima K, Takeshita T, Yamada M. Angiotensin II activates CaV 1.2 Ca2+ channels through ?-arrestin2 and casein kinase 2 in mouse immature cardiomyocytes. J Physiol. 2017;595:4207-4225 pubmed 出版商
Zhai Z, Liu W, Kaur M, Luo Y, Domenico J, Samson J, et al. NLRP1 promotes tumor growth by enhancing inflammasome activation and suppressing apoptosis in metastatic melanoma. Oncogene. 2017;36:3820-3830 pubmed 出版商
Seong H, Manoharan R, Ha H. Zinc finger protein ZPR9 functions as an activator of AMPK-related serine/threonine kinase MPK38/MELK involved in ASK1/TGF-β/p53 signaling pathways. Sci Rep. 2017;7:42502 pubmed 出版商
Nounamo B, Li Y, O Byrne P, Kearney A, Khan A, Liu J. An interaction domain in human SAMD9 is essential for myxoma virus host-range determinant M062 antagonism of host anti-viral function. Virology. 2017;503:94-102 pubmed 出版商
Mansilla A, Chaves Sanjuan A, Campillo N, Semelidou O, Martínez González L, Infantes L, et al. Interference of the complex between NCS-1 and Ric8a with phenothiazines regulates synaptic function and is an approach for fragile X syndrome. Proc Natl Acad Sci U S A. 2017;114:E999-E1008 pubmed 出版商
Rivera Serrano E, Fritch E, Scholl E, Sherry B. A Cytoplasmic RNA Virus Alters the Function of the Cell Splicing Protein SRSF2. J Virol. 2017;91: pubmed 出版商
Urbanus M, Quaile A, Stogios P, Morar M, Rao C, Di Leo R, et al. Diverse mechanisms of metaeffector activity in an intracellular bacterial pathogen, Legionella pneumophila. Mol Syst Biol. 2016;12:893 pubmed 出版商
Zhang Y, Li Q, Youn J, Cai H. Protein Phosphotyrosine Phosphatase 1B (PTP1B) in Calpain-dependent Feedback Regulation of Vascular Endothelial Growth Factor Receptor (VEGFR2) in Endothelial Cells: IMPLICATIONS IN VEGF-DEPENDENT ANGIOGENESIS AND DIABETIC WOUND HEALING. J Biol Chem. 2017;292:407-416 pubmed 出版商
Jeganathan N, Predescu D, Zhang J, Sha F, Bardita C, Patel M, et al. Rac1-mediated cytoskeleton rearrangements induced by intersectin-1s deficiency promotes lung cancer cell proliferation, migration and metastasis. Mol Cancer. 2016;15:59 pubmed 出版商
Jungas T, Perchey R, Fawal M, Callot C, Froment C, Burlet Schiltz O, et al. Eph-mediated tyrosine phosphorylation of citron kinase controls abscission. J Cell Biol. 2016;214:555-69 pubmed 出版商
McCormick S, Gowda N, Fang J, Heller N. Suppressor of Cytokine Signaling (SOCS)1 Regulates Interleukin-4 (IL-4)-activated Insulin Receptor Substrate (IRS)-2 Tyrosine Phosphorylation in Monocytes and Macrophages via the Proteasome. J Biol Chem. 2016;291:20574-87 pubmed 出版商
Min Y, Wi S, Kang J, Yang T, Park C, Park S, et al. Cereblon negatively regulates TLR4 signaling through the attenuation of ubiquitination of TRAF6. Cell Death Dis. 2016;7:e2313 pubmed 出版商
Luo H, Cowen L, Yu G, Jiang W, Tang Y. SMG7 is a critical regulator of p53 stability and function in DNA damage stress response. Cell Discov. 2016;2:15042 pubmed 出版商
Aly H, Suzuki J, Watashi K, Chayama K, Hoshino S, Hijikata M, et al. RNA Exosome Complex Regulates Stability of the Hepatitis B Virus X-mRNA Transcript in a Non-stop-mediated (NSD) RNA Quality Control Mechanism. J Biol Chem. 2016;291:15958-74 pubmed 出版商
Akiyama H, Fukuda T, Tojima T, Nikolaev V, Kamiguchi H. Cyclic Nucleotide Control of Microtubule Dynamics for Axon Guidance. J Neurosci. 2016;36:5636-49 pubmed 出版商
Zhang Y, Lee K, Kinch L, Clark L, Grishin N, Rosenbaum D, et al. Direct Demonstration That Loop1 of Scap Binds to Loop7: A CRUCIAL EVENT IN CHOLESTEROL HOMEOSTASIS. J Biol Chem. 2016;291:12888-96 pubmed 出版商
Saito M, Tanaka M, Misawa E, Yao R, Nabeshima K, Yamauchi K, et al. Oral administration of Aloe vera gel powder prevents UVB-induced decrease in skin elasticity via suppression of overexpression of MMPs in hairless mice. Biosci Biotechnol Biochem. 2016;80:1416-24 pubmed 出版商
Kenific C, Stehbens S, Goldsmith J, Leidal A, Faure N, Ye J, et al. NBR1 enables autophagy-dependent focal adhesion turnover. J Cell Biol. 2016;212:577-90 pubmed 出版商
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St Denis N, Gupta G, Lin Z, Gonzalez Badillo B, Pelletier L, Gingras A. Myotubularin-related proteins 3 and 4 interact with polo-like kinase 1 and centrosomal protein of 55 kDa to ensure proper abscission. Mol Cell Proteomics. 2015;14:946-60 pubmed 出版商
目录号 :
名称 :
Mouse TrueBlot® ULTRA: Anti-Mouse Ig HRP - 18-8817-31
显示名称 :
Mouse TrueBlot® ULTRA: Anti-Mouse Ig HRP
应用注释 :
Mouse TrueBlot® ULTRA has been reported for use in IP/immunoblot and immunoblotting (WB). Mouse IgG TrueBlot® ULTRA is provided as 1000X solution. To achieve best results when detecting mouse IgG1 subtypes, we recommend performing a dot blot or titration to determine the optimal dilution factor for your desired application. All recommended dilutions for listed applications are intended as an initial recommendation, specific conditions for each protein and antibody combination should be specifically optimized by the end user. To conserve reagent, we recommend incubating the blots from minigels in sealed bags (removing as much air as possible) with minimal volume (2-3 mLs). If used conservatively at 2.5mls/blot will yield enough reagent for 20 blots. Note that there are three key procedural considerations: 1. Protein A or G beads may be used with the mouse, goat and sheep TrueBlot secondaries, but not with the rabbit TrueBlot secondary. Use of protein A or G beads with the rabbit TrueBlot will result in contaminating bands. 2. Immunoprecipitate should be completely reduced. 3. BLOTTO/Milk should be used as the blocking protein for the immunoblot.
缓冲液 :
0.02 M钾磷酸盐, 0.15 M氯化钠, pH值7.2
克隆性 :
克隆编号 :
浓度值 :
浓度定义 :
by UV absorbance at 280 nm
共轭 :
规格 :
默认单元 :
免疫印迹稀释浓度 :
有效期 :
Expiration date is six (6) months from date of opening.
格式 :
宿主动物 :
一般免责声明 :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
包装类型 :
Wet Ice
物理状态 :
Liquid (sterile filtered)
纯度和特异性 :
MouseTrueBlot® ULTRA Antibody Peroxidase Conjugate was prepared from tissue culture supernatant by Protein G affinity chromatography. Assay by Immunoelectrophoresis resulted in a single precipitin arc against Anti-Mouse Serum. Reactivity is observed against native Mouse IgG by both Western blot and ELISA.
物种活性 :
稳定剂 :
0.1毫克/毫升牛血清白蛋白(BSA)-不含IgG和蛋白酶, 50%(v/v)甘油
储存 :
Store at -20 °C. This product is guaranteed for 1 year upon receipt, when handled and stored as instructed.
同义词 :
Anti-Mouse IgG HRP, TrueBlot, HRP TrueBlot ULTRA, Peroxidase TrueBlot, TrueBlot for IP/WB, TrueBlot for immunoprecipitation, TrueBlot for western blotting
靶物种 :
背景 :
Mouse IgG TrueBlot® ULTRA is a unique anti-mouse IgG monoclonal secondary antibody. Mouse IgG TrueBlot® ULTRA enables detection of immunoblotted target protein bands, without hindrance by interfering immunoprecipitating immunoglobulin heavy and light chains. It is easy to generate publication-quality IP/Western blot data with Mouse IgG TrueBlot® ULTRA, simply substitute the conventional Anti-Mouse IgG blotting reagent with Mouse IgG TrueBlot® ULTRA and follow the prescribed protocol for sample preparation and immunoblotting. Mouse IgG TrueBlot® ULTRA is ideal for use in protocols involving immunoblotting of immunoprecipitated proteins. Mouse IgG TrueBlot® ULTRA preferentially detects the non-reduced form of mouse IgG (IgG1, IgG2a, IgG2b, IgG3) over the reduced, SDS-denatured form of IgG. When the immunoprecipitate is fully reduced immediately prior to SDS-gel electrophoresis, reactivity of Mouse IgG TrueBlot® ULTRA with the 55 kDa heavy chains and the 23 kDa light chains of the immunoprecipitating antibody is minimized thereby eliminating interference by the heavy and light chains of the immunoprecipitating antibody in IP/immunoblot applications. Applications include studies examining post-translational modification (e.g., phosphorylation or acetylation) or protein-protein interactions.
检测试剂盒类型 :
Immunoprecipitation Kit
检测试剂盒和组成 :
低内毒素 :
样品尺寸 :
应用文本 :
类别 :
共轭名称 :
HRP TrueBlot® ULTRA for IP/WB
2020价格 :
最初图像名称 :
Mouse TrueBlot® IP / Western Blot
更多信息或购买 :
Rockland Immunochemicals
321 Jones Blvd
Pottstown, PA 19464
公司总部: 美国
Rockland Immunochemicals, Inc.位于宾夕法尼亚州的费城西部,为基础研究、应用研究及临床研究提供磷酸化位点特异性抗体及抗体相关研究工具。公司的技术平台是由一批经验丰富的科学家建立,利用In vitro及In vivo方法制备并纯化单抗及多抗,这些抗体应用于免疫印迹、ELISA、免疫组化、荧光检测、高通量筛选及诊断试剂盒生产等。常用一抗主要应用于以下研究:Akt通路、载脂蛋白、细胞凋亡/细胞周期、细胞因子、细胞信号、酶类、胞外基质、转录因子及泛素相关领域。