产品简要
公司名称 :
Rockland Immunochemicals
产品类型 :
抗体
产品名称 :
Cdc2 p34抗体
目录 :
200-301-160
规格 :
100
价格 :
https://www.rockland.com/search/?searchString=200-301-160
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
POH-1
反应物种 :
人类
应用 :
免疫印迹, 酶联免疫吸附测定, 免疫沉淀
更多信息或购买 :
产品信息
目录号 :
200-301-160
名称 :
抗cdc2(p34)(小鼠)单克隆抗体-200-301
显示名称 :
Cdc2 p34抗体
应用注释 :
Anti-p34 cdc2 has been tested by western blot and is suitable for immunohistochemistry, immunoprecipitation (as active kinase), and immunoblotting. The antibody detects the three bands within the 34kD region corresponding to the p34 protein and its cleavage products.  HeLa cell lysate or human colon carcinoma is suggested as a positive control for immunoblotting.  LEP fibroblast cell lysate is suggested as a negative control.   Paraffin embedded tissue is reactive for immunohistochemistry using high temperature release and 0.1% saponin treatment or other permeabilization method.
缓冲液 :
0.02 M钾磷酸盐, 0.5 M氯化钠, pH值7.2
克隆性 :
单克隆
克隆编号 :
POH-1
浓度值 :
1.1毫克/毫升
浓度定义 :
by UV absorbance at 280 nm
共轭 :
(None)
规格 :
100
默认单元 :
微克
ELISA稀释浓度 :
1:5, 000 - 1:20, 000
免疫沉淀稀释度 :
1:100
免疫印迹稀释浓度 :
1:500 - 1:1, 000
有效期 :
Expiration date is one (1) year from date of receipt.
格式 :
IgG2a
基因名称 :
CDK1
宿主动物 :
小鼠
一般免责声明 :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
免疫原 :
This protein A purified monoclonal antibody was produced by repeated immunizations with recombinant human p34 cdc2 fusion protein.
包装类型 :
干冰
物理状态 :
Liquid (sterile filtered)
防腐剂 :
0.01%(w/v)叠氮化钠
纯度和特异性 :
This protein A purified mouse monoclonal antibody reacts specifically with p34 cdc2 in human tissues and cell lines.  This antibody is not cross reactive with other cyclin dependent kinases.  Cross reactivity with p34 cdc2 from other sources, especially mouse and rat will occur.  This reagent has broad interspecies reactivity.
物种活性 :
人类
检测过的应用 :
免疫印迹
储存 :
Store vial at -20° C prior to opening.  Aliquot contents and freeze at -20° C or below for extended storage.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.
子种类 :
IgG2a kappa
同义词 :
小鼠抗Cdc2抗体, 小鼠抗p34抗体, 细胞分裂周期2蛋白抗体, 细胞周期蛋白依赖性激酶 1抗体, p34 Cdk1抗体, p34蛋白激酶抗体, CDC28A, CDKN1, P34CDC2
靶物种 :
人类
背景 :
p34 cdc2 is a serine-threonine protein kinase of 34,000 daltons that complexes with cyclin to form maturation promoting factor (MPF).  The inactive form of the protein is phosphorylated at threonine (T) and tyrosine (Y) residues.  In humans the phosphorylation appears to be performed by p60src.  The active form of the protein is dephosphorylated and it functions by phosphorylating a number of proteins.  The phosphorylation activity is coupled to the entry into the M-phase of the cell.  p34 cdc2 protein must be associated with a normal cyclin protein for the M-phase to be completed normally.  Association with deletion mutants of cyclin halts the M-phase before it is completed.
免疫原类型 :
重组蛋白
低内毒素 :
No
样品尺寸 :
No
应用文本 :
酶联免疫吸附测定, 免疫沉淀, 免疫印迹
其他 :
User Optimized
类别 :
一抗
共轭名称 :
未共轭
2024价格 :
$485.00
UniProt数据库 :
P06493
NCBI :
NP_001307847.1
最初图像名称 :
抗人类p34cdc2单克隆抗体-免疫印迹
更多信息或购买 :
公司信息
Rockland Immunochemicals
321 Jones Blvd
Pottstown, PA 19464
orders@rockland-inc.com
https://www.rockland.com/
800-656-7625
公司总部: 美国
Rockland Immunochemicals, Inc.位于宾夕法尼亚州的费城西部,为基础研究、应用研究及临床研究提供磷酸化位点特异性抗体及抗体相关研究工具。公司的技术平台是由一批经验丰富的科学家建立,利用In vitro及In vivo方法制备并纯化单抗及多抗,这些抗体应用于免疫印迹、ELISA、免疫组化、荧光检测、高通量筛选及诊断试剂盒生产等。常用一抗主要应用于以下研究:Akt通路、载脂蛋白、细胞凋亡/细胞周期、细胞因子、细胞信号、酶类、胞外基质、转录因子及泛素相关领域。