抗体

p21 WAF1抗体

200-301-171

100微克

单克隆

小鼠

未共轭

WA-1

免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫沉淀

抗p21/WAF1(小鼠)单克隆抗体-200-301

p21 WAF1抗体

This antibody is suitable for immunohistochemistry, ELISA, immunofluorescence, and immunoblotting. The antibody detects a 21 kDa band corresponding to the p21 protein.  MCF7 cells or UV treated fibroblasts are suggested as a positive control for immunoblotting and immunohistochemistry.   Methanol/acetone fixed tissue is reactive for immunohistochemistry. The antibody also reacts with breast carcinoma on frozen sections (methanol/acetone fixation) and on paraffin sections (formalin fixation and microwave treatment prior to staining).  In ELISA this monoclonal antibody is useful either as a capture antibody (attached to the solid phase) or for detection of p21 protein.  This antibody immunoprecipitates monomeric p21WAF1 and not p21 complexes with other proteins. High level expression of p21WAF1 requires induction of wild-type p53 expression (e.g. via exposure to DNA damaging agents).

0.02 M钾磷酸盐, 0.5 M氯化钠, pH值7.2

单克隆

WA-1

1.0毫克/毫升

by UV absorbance at 280 nm

(None)

100微克

微克

1:5, 000 - 1:20, 000

User Optimized

1:250

1:100

1:2, 000 - 1:10, 000

Expiration date is one (1) year from date of opening.

IgG1

CDKN1A

小鼠

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

This protein A purified monoclonal antibody was produced by repeated immunizations with recombinant human p21 fusion protein.

干冰

Liquid (sterile filtered)

0.01%(w/v)叠氮化钠

This protein A purified mouse monoclonal antibody reacts with human and mammalian p21. The intracellular protein p21 is a 21 kD protein and also known as wild-type p53-activated fragment 1 (WAF1).  It is an inhibitor of cyclin-dependent kinases (Cdks) and proliferating-cell nuclear antigen (PCNA). The antibody is highly specific and shows no cross-reactivity with other closely related mitotic inhibitors.

人类

Store vial at -20° C prior to opening.  Aliquot contents and freeze at -20° C or below for extended storage.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.

IgG1 kappa

mouse anti-p21/WAF1 antibody, Cyclin-dependent kinase inhibitor 1A (p21, CIP1) antibody, DNA Synthesis Inhibitor antibody, MDA 6 antibody, MDA6 antibody, Melanoma Differentiation Associated Protein 6 antibody

人类

p21WAF1 is a tumor suppressor gene product critical to maintain proper regulatory control of cell division. p21WAF1 inhibits cyclin/cyclin-dependent kinase (cdk) complexes, causing cell cycle arrest by blocking cell division at the S phase. Various mutations of tumor suppressor genes have been associated with different types of cancers. In these cases, somatic gene therapy with wild-type versions of tumor suppressor genes have been contemplated as anti-cancer therapeutic approaches. p21WAF1 contains p53-binding sites in its promoter and expression of p21WAF1 is induced in WI38 fibroblasts with PMA. Anti-p21WAF1 antibody is useful for researches interested in studying tongue and gastrointestinal cancers, as well as, ubiquitin and cyclin binding proteins.

重组蛋白

No

No

酶联免疫吸附测定, 免疫组化, IF显微镜, 免疫沉淀, 免疫印迹

User Optimized

一抗

未共轭

P38936

NP_510867.1

抗人类p21WAF1单克隆抗体-免疫印迹