产品简要
公司名称 :
Rockland Immunochemicals
产品类型 :
抗体
产品名称 :
p53抗体
目录 :
200-301-174
规格 :
100微克
价格 :
369.00美元
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
BP53-12
反应物种 :
人类
应用 :
免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫沉淀, 染色质免疫沉淀
更多信息或购买 :
产品信息
目录号 :
200-301-174
名称 :
抗p53(小鼠)单克隆抗体-200-301
显示名称 :
p53抗体
应用注释 :
This antibody is suitable for ChIP, flow cytometry, immunohistochemistry, Immunofluorescence, immunoblotting and immunoprecipitation.  p53 is the most commonly mutated gene in spontaneously occurring human cancers. Mutations arise with an average frequency of 70% but incidence varies from zero in carcinoid lung tumors to 97% in primary melanomas.  High concentrations of p53 protein are transiently expressed in human epidermis and superficial dental fibroblasts following mild ultraviolet irradiation. This antibody reacts with an N-terminal epitope of the 53 kD gene product and this epitope is not destroyed by formalin-fixation and routine paraffin embedding. Microwaving is needed for optimal staining.
缓冲液 :
0.02 M钾磷酸盐, 0.5 M氯化钠, pH值7.2
克隆性 :
单克隆
克隆编号 :
BP53-12
浓度值 :
1.0毫克/毫升
浓度定义 :
by UV absorbance at 280 nm
共轭 :
(None)
规格 :
100微克
默认单元 :
微克
ELISA稀释浓度 :
1:2, 000 - 1:10, 000
染色质免疫共沉淀稀释 :
1 µg/µL at 4° o/n
免疫组化稀释度 :
1:50
免疫荧光显微镜稀释浓度 :
1:100-1:500
免疫沉淀稀释度 :
1:100
免疫印迹稀释浓度 :
1:500 - 1:2, 000
有效期 :
Expiration date is one (1) year from date of opening.
格式 :
IgG2a
基因名称 :
TP53
宿主动物 :
小鼠
一般免责声明 :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
免疫原 :
This protein A purified monoclonal antibody was produced by repeated immunizations with recombinant human p53 protein.
包装类型 :
干冰
物理状态 :
Liquid (sterile filtered)
防腐剂 :
0.01%(w/v)叠氮化钠
纯度和特异性 :
This protein A purified mouse monoclonal antibody reacts specifically with p53 in human tissues and cell lines.  The antibody recognizes a 53 kDa band corresponding to p53.  Cross reactivity with p53 from other sources has not been determined.
物种活性 :
人类
储存 :
Store vial at -20° C prior to opening.  Aliquot contents and freeze at -20° C or below for extended storage.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.
子种类 :
IgG2a kappa
同义词 :
mouse anti-p53 antibody, mouse anti-Tumor Suppressor p53 antibody, Phosphoprotein p53 antibody, TP53 antibody, Transformation related protein 53 antibody, TRP53 antibody, cellular Tumor antigen p53 antibody
靶物种 :
人类
背景 :
The p53 gene like the Rb gene, is a tumor suppressor gene. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism; its activity stops the formation of tumors. If a person inherits only one functional copy of the p53 gene from their parents, they are pre-disposed to cancer and usually develop several independent tumors in a variety of tissues in early adulthood. This condition is rare, and is known as Li-Fraumeni syndrome. However, mutations in p53 are found in most tumor types, and so contribute to the complex network of molecular events leading to tumor formation. Anti-p53 Antibody is ideal for investigators involved in Signaling Proteins, Cell Cycle Proteins, Apoptosis/Autophagy, Cancer, Cardiovascular Disease, Cell Cycle, Cellular Stress, Inflammation, JNK/SAPK Pathway, Metabolic Disorder, Neurobiology, and p38 Pathway research.
免疫原类型 :
重组蛋白
低内毒素 :
No
样品尺寸 :
No
应用文本 :
ELISA,Chip,Immunohistochemistry,IF Microscopy,Immunoprecipitation,Western Blot,
其他 :
User Optimized
类别 :
一抗
共轭名称 :
未共轭
2020价格 :
369.00美元
UniProt数据库 :
P04637
NCBI :
23491729
最初图像名称 :
p53抗体-免疫荧光显微镜
更多信息或购买 :
公司信息
Rockland Immunochemicals
321 Jones Blvd
Pottstown, PA 19464
orders@rockland-inc.com
https://rockland-inc.com
800-656-7625
公司总部: 美国
Rockland Immunochemicals, Inc.位于宾夕法尼亚州的费城西部,为基础研究、应用研究及临床研究提供磷酸化位点特异性抗体及抗体相关研究工具。公司的技术平台是由一批经验丰富的科学家建立,利用In vitro及In vivo方法制备并纯化单抗及多抗,这些抗体应用于免疫印迹、ELISA、免疫组化、荧光检测、高通量筛选及诊断试剂盒生产等。常用一抗主要应用于以下研究:Akt通路、载脂蛋白、细胞凋亡/细胞周期、细胞因子、细胞信号、酶类、胞外基质、转录因子及泛素相关领域。