抗体

HSP27抗体

200-301-243

100微克

单克隆

小鼠

未共轭

G3.1

免疫印迹, 酶联免疫吸附测定

抗HSP27(小鼠)单克隆抗体-200-301

HSP27抗体

This protein A purified monoclonal antibody against human Hsp27 has been tested for use in immunoblotting, immunoprecipitation, ELISA, immunohistochemistry and immunocytochemistry.  The antibody recognizes a 27 kDa band corresponding to hsp27 in cell lysates from breast carcinoma.    Both frozen sections and paraffin embedded material can be used for immunocytochemistry and immunohistochemistry.

0.02 M钾磷酸盐, 0.5 M氯化钠, pH值7.2

单克隆

G3.1

1.0毫克/毫升

by UV absorbance at 280 nm

(None)

100微克

微克

1:10, 000 - 1:50, 000

1:500 - 1:1, 000

Expiration date is one (1) year from date of opening.

IgG1

HSPB1

小鼠

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

This HSP27 monoclonal antibody was produced by repeated immunizations with a prokaryotic recombinant protein corresponding to the full length human hsp27 protein.

干冰

Liquid (sterile filtered)

0.01%(w/v)叠氮化钠

This protein A purified mouse monoclonal antibody reacts specifically with HSP27 in human tissues and cell lines.  MCF-7 cells are recommended as a positive control.  Cross reactivity with hsp27 from other mammalian sources is likely.  No cross reactivity occurs with HSP70, HSP90 or HSP104.

人类

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

IgG1 kappa

mouse anti-HSP27 antibody, mouse anti-Heat shock protein beta-1 antibody, HspB1 antibody, Heat shock 27 kDa protein antibody, Stress-responsive protein 27 antibody, SRP27 antibody, Estrogen-regulated 24 kDa protein antibody, 28 kDa heat shock protein antibody

人类

Heat shock protein (HSP) 27 is one of the small HSPs that are constitutively expressed at different levels in different cell types and tissues (this protein has also been referred to as the Estrogen-Regulated 24 kDa protein, hsp25 and hsp28). Like other small heat shock proteins, HSP27 is regulated at both the transcriptional and post-translational level. In response to stress, the expression level of HSP27 increases several-fold to confer cellular resistance to the adverse environmental change. The common functions of sHsps are chaperone activity, thermotolerance, inhibition of apoptosis, regulation of cell development, and cell differentiation. They also take part in signal transduction. The HSP27 gene has 3 exons. The mouse Hsp25 gene was mapped to chromosome 5 in a region homologous to 7q in the human. They also mapped the mouse Hsp105 gene to chromosome 5 but suggested that the human homolog is probably on 13q, not chromosome 7. HSP27 plays a major role in the increased thermal resistance acquired by cells after exposure to HSP inducers. The level of HSP27 phosphorylation is significantly elevated after exposure of cells to heat shock, sodium arsenite, IL-1 and TNF-a. MAPKAPK2 and MAPKAPK3 are both activated by these conditions and can phosphorylate HSP27 on serine residues Anti-HSP27 Antibody is ideal for investigators involved in Signaling Proteins, Cell Stress & Chaperone Proteins, Cancer, Cellular Stress, and p38 Pathway research.

重组蛋白

No

No

酶联免疫吸附测定, 免疫印迹

User Optimized

一抗

未共轭

P04792

17647521

抗人类HSP27单克隆抗体-免疫印迹