产品简要
公司名称 :
Rockland Immunochemicals
产品类型 :
抗体
产品名称 :
AKT磷酸化S473抗体
目录 :
200-301-268
规格 :
100微克
价格 :
369美元
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
17F6.B11
反应物种 :
人类, 小鼠, 大鼠
应用 :
免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫沉淀, 流式细胞仪
更多信息或购买 :
文章摘录数: 1
出版应用/物种/样本/稀释参考文献
  • 免疫组化; 小鼠; 1:500; 图 6
Sha W, Thompson K, South J, Baron M, Leask A. Loss of PPAR? expression by fibroblasts enhances dermal wound closure. Fibrogenesis Tissue Repair. 2012;5:5 pubmed 出版商
产品信息
目录号 :
200-301-268
名称 :
抗AKT pS473(小鼠)单克隆抗体-200-301
显示名称 :
AKT磷酸化S473抗体
应用注释 :
Phospho AKT antibody is suitable for ELISA, immunofluorescence, immunohistochemistry, immunoprecipitation and western blotting. Expect a band approximately 56 kDa in size corresponding to phosphorylated AKT protein by western blotting in the appropriate cell lysate or extract. This phospho-specific monoclonal antibody reacts with human and mouse AKT pS473 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide. Specific conditions for reactivity should be optimized by the end user. For immunohistochemistry use formalin-fixed paraffin-embedded sections. No pre-treatment of sample is required.
缓冲液 :
0.02 M钾磷酸盐, 0.15 M氯化钠, pH值7.2
克隆性 :
单克隆
克隆编号 :
17F6.B11
浓度值 :
1.0毫克/毫升
浓度定义 :
by UV absorbance at 280 nm
共轭 :
(None)
规格 :
100微克
默认单元 :
微克
ELISA稀释浓度 :
1:20,000
流式细胞仪稀释浓度 :
User Optimized
免疫组化稀释度 :
20微克/毫升
免疫荧光显微镜稀释浓度 :
1:500-1:3,000
免疫沉淀稀释度 :
User Optimized
免疫印迹稀释浓度 :
1:500-1:3,000
有效期 :
Expiration date is one (1) year from date of opening.
格式 :
IgG1
基因名称 :
AKT1
宿主动物 :
小鼠
一般免责声明 :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
免疫原 :
Anti-AKT pS473 (MOUSE) Monoclonal Antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding S473 of human AKT1 protein, followed by hybridoma development.
包装类型 :
干冰
物理状态 :
Liquid (sterile filtered)
防腐剂 :
0.01%(w/v)叠氮化钠
纯度和特异性 :
Anti-AKT pS473 Monoclonal Antibody was purified from concentrated tissue culture supernate by Protein A chromatography. This phospho specific monoclonal antibody is specific for phosphorylated human and mouse AKT protein at S473. A BLAST analysis was used to suggest cross-reactivity with AKT pS473 from human, mouse, rat and chimpanzee sources based on 100% homology with the immunizing sequence. Cross-reactivity with AKT from other sources has not been determined. Cross-reactivity with AKT2 and AKT3 has not been determined.
物种活性 :
human, mouse, rat, monkey
储存 :
Store Anti-AKT pS473 (MOUSE) Monoclonal Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
子种类 :
IgG1 kappa
同义词 :
mouse anti-AKT pS473 Antibody, phospho AKT, RAC-PK-alpha, Protein kinase B, PKB, C-AKT, RAC-alpha serine/threonine-protein kinase, Proto-oncogene c-Akt, AKT1, AKT 1, AKT-1
靶物种 :
人类
背景 :
AKT phospho S473 is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308. AKT is a cytoplasmic protein also known as AKT1, Protein Kinase B (PKB) and rac (related to A and C kinases). AKT is a key regulator of many signal transduction pathways. AKT Exhibits tight control over cell proliferation and cell viability. Overexpression or inappropriate activation of AKT is noted in many types of cancer. AKT mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. AKT has two main roles: (i) inhibition of apoptosis; (ii) promotion of proliferation. Anti-AKT pS473 (MOUSE) Monoclonal Antibody is ideal for investigators involved in Cell Signaling, Cancer, Neuroscience, Signal Transduction research.
一般参考 :
Lawlor, M. A. and Alessi, D.R. (2001). PKB/AKT: a key mediator of cell proliferation, survival and insulin responses. J. Cell Science 114:2903-2910. Alessi, D. R. (2001). Discovery of PDK1, one of the missing links in insulin signal transduction. Biochem. Soc. Trans. 29,1 -14. Jones,P.F., Jakubowicz,T., Pitossi,F.J., Maurer,F. and Hemmings,B.A. (1991) Molecular cloning and identification of a serine/threonine protein kinase of the second-messenger subfamily. Proc. Natl. Acad. Sci. U.S.A. 88 (10), 4171-4175.
特定参考文献 :
Sato et al. (2017) Sodium butyrate enhances the growth inhibitory effect of sunitinib in human renal cell carcinoma cells. Oncol Lett. Jul;14(1):937-943. PMID: 28693255 - IP.
免疫原类型 :
多肽
低内毒素 :
No
翻译后的修饰作用 :
磷酸化
样品尺寸 :
No
靶活性结构域 :
内部
应用文本 :
酶联免疫吸附测定, 流式细胞仪, 免疫组化, IF显微镜, 免疫沉淀, 免疫印迹
其他 :
User Optimized
类别 :
一抗
共轭名称 :
未共轭
2020价格 :
369美元
UniProt数据库 :
P31749
NCBI :
62241011
最初图像名称 :
AKT pS473 Monoclonal Antibody – Immunohistochemistry
更多信息或购买 :
公司信息
Rockland Immunochemicals
321 Jones Blvd
Pottstown, PA 19464
orders@rockland-inc.com
https://rockland-inc.com
800-656-7625
公司总部: 美国
Rockland Immunochemicals, Inc.位于宾夕法尼亚州的费城西部,为基础研究、应用研究及临床研究提供磷酸化位点特异性抗体及抗体相关研究工具。公司的技术平台是由一批经验丰富的科学家建立,利用In vitro及In vivo方法制备并纯化单抗及多抗,这些抗体应用于免疫印迹、ELISA、免疫组化、荧光检测、高通量筛选及诊断试剂盒生产等。常用一抗主要应用于以下研究:Akt通路、载脂蛋白、细胞凋亡/细胞周期、细胞因子、细胞信号、酶类、胞外基质、转录因子及泛素相关领域。