产品简要
公司名称 :
Rockland Immunochemicals
产品类型 :
抗体
产品名称 :
毛细血管扩张性共济失调症突变蛋白磷酸化S1981抗体
目录 :
200-301-400
规格 :
100
价格 :
499.00美元
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
抗原修饰 :
磷酸化
克隆名称 :
10H11.E12
反应物种 :
人类, 小鼠, 大鼠
应用 :
免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫细胞化学, 免疫沉淀, 流式细胞仪, 免疫印迹基因敲除验证
更多信息或购买 :
文章摘录数: 71
出版应用/物种/样本/稀释参考文献
  • 免疫印迹; 小鼠; 1:1000; 图 s5m
Hansen R, Mund A, Poulsen S, Sandoval M, Klement K, Tsouroula K, et al. SCAI promotes DNA double-strand break repair in distinct chromosomal contexts. Nat Cell Biol. 2016;18:1357-1366 pubmed 出版商
  • 免疫细胞化学; 人类; 图 2c
Bakr A, Köcher S, Volquardsen J, Petersen C, Borgmann K, Dikomey E, et al. Impaired 53BP1/RIF1 DSB mediated end-protection stimulates CtIP-dependent end resection and switches the repair to PARP1-dependent end joining in G1. Oncotarget. 2016;7:57679-57693 pubmed 出版商
  • 免疫细胞化学; 人类; 1:500; 图 5
Qi Y, Qiu Q, Gu X, Tian Y, Zhang Y. ATM mediates spermidine-induced mitophagy via PINK1 and Parkin regulation in human fibroblasts. Sci Rep. 2016;6:24700 pubmed 出版商
  • 免疫印迹; 人类; 图 3h
Torres M, Pandita R, Kulak O, Kumar R, Formstecher E, Horikoshi N, et al. Role of the Exocyst Complex Component Sec6/8 in Genomic Stability. Mol Cell Biol. 2015;35:3633-45 pubmed 出版商
  • 免疫印迹; 小鼠; 图 s5
Hartlerode A, Morgan M, Wu Y, Buis J, Ferguson D. Recruitment and activation of the ATM kinase in the absence of DNA-damage sensors. Nat Struct Mol Biol. 2015;22:736-43 pubmed 出版商
  • 免疫细胞化学; 小鼠
Xiong J, Todorova D, Su N, Kim J, Lee P, Shen Z, et al. Stemness factor Sall4 is required for DNA damage response in embryonic stem cells. J Cell Biol. 2015;208:513-20 pubmed 出版商
  • 免疫印迹; 人类
Xue L, Furusawa Y, Okayasu R, Miura M, Cui X, Liu C, et al. The complexity of DNA double strand break is a crucial factor for activating ATR signaling pathway for G2/M checkpoint regulation regardless of ATM function. DNA Repair (Amst). 2015;25:72-83 pubmed 出版商
  • 免疫印迹; 人类; 1:500; 图 3a
Wei F, Ojo D, Lin X, Wong N, He L, Yan J, et al. BMI1 attenuates etoposide-induced G2/M checkpoints via reducing ATM activation. Oncogene. 2015;34:3063-75 pubmed 出版商
  • 免疫印迹; 人类; 图 s1
Smith C, Matheson T, Trombly D, Sun X, Campeau E, Han X, et al. A separable domain of the p150 subunit of human chromatin assembly factor-1 promotes protein and chromosome associations with nucleoli. Mol Biol Cell. 2014;25:2866-81 pubmed 出版商
  • 免疫细胞化学; 人类
Manguan García C, Pintado Berninches L, Carrillo J, Machado Pinilla R, Sastre L, Perez Quilis C, et al. Expression of the genetic suppressor element 24.2 (GSE24.2) decreases DNA damage and oxidative stress in X-linked dyskeratosis congenita cells. PLoS ONE. 2014;9:e101424 pubmed 出版商
  • 免疫印迹基因敲除验证; 小鼠; 图 2
Shamma A, Suzuki M, Hayashi N, Kobayashi M, Sasaki N, Nishiuchi T, et al. ATM mediates pRB function to control DNMT1 protein stability and DNA methylation. Mol Cell Biol. 2013;33:3113-24 pubmed 出版商
  • 免疫细胞化学; 人类
Birkelbach M, Ferraiolo N, Gheorghiu L, Pfäffle H, Daly B, Ebright M, et al. Detection of impaired homologous recombination repair in NSCLC cells and tissues. J Thorac Oncol. 2013;8:279-86 pubmed 出版商
  • 免疫细胞化学; 人类; 1:50
Wu L, Shao L, Li M, Zheng J, Wang J, Feng W, et al. BMS-345541 sensitizes MCF-7 breast cancer cells to ionizing radiation by selective inhibition of homologous recombinational repair of DNA double-strand breaks. Radiat Res. 2013;179:160-70 pubmed 出版商
  • 免疫印迹; 小鼠; 1:1000
Pusapati R, Rounbehler R, Hong S, Powers J, Yan M, Kiguchi K, et al. ATM promotes apoptosis and suppresses tumorigenesis in response to Myc. Proc Natl Acad Sci U S A. 2006;103:1446-51 pubmed
Zhai B, Li Y, Kotapalli S, Bacha J, Brown D, Steinø A, et al. Dianhydrogalactitol synergizes with topoisomerase poisons to overcome DNA repair activity in tumor cells. Cell Death Dis. 2020;11:577 pubmed 出版商
Zhu C, Rogers A, Asleh K, Won J, Gao D, Leung S, et al. Phospho-Ser784-VCP Is Required for DNA Damage Response and Is Associated with Poor Prognosis of Chemotherapy-Treated Breast Cancer. Cell Rep. 2020;31:107745 pubmed 出版商
Zhao J, Zhang L, Lu A, Han Y, Colangelo D, Bukata C, et al. ATM is a key driver of NF-κB-dependent DNA-damage-induced senescence, stem cell dysfunction and aging. Aging (Albany NY). 2020;12:4688-4710 pubmed 出版商
Kreis P, Gallrein C, Rojas Puente E, Mack T, Kroon C, Dinkel V, et al. ATM phosphorylation of the actin-binding protein drebrin controls oxidation stress-resistance in mammalian neurons and C. elegans. Nat Commun. 2019;10:486 pubmed 出版商
Li C, Mahon C, Sweeney N, Verschueren E, Kantamani V, Li D, et al. PPARγ Interaction with UBR5/ATMIN Promotes DNA Repair to Maintain Endothelial Homeostasis. Cell Rep. 2019;26:1333-1343.e7 pubmed 出版商
Carranza D, Torres Rusillo S, Ceballos Pérez G, Blanco Jimenez E, Muñoz Lopez M, Garcia Perez J, et al. Reconstitution of the Ataxia-Telangiectasia Cellular Phenotype With Lentiviral Vectors. Front Immunol. 2018;9:2703 pubmed 出版商
Fujiwara C, Muramatsu Y, Nishii M, Tokunaka K, Tahara H, Ueno M, et al. Cell-based chemical fingerprinting identifies telomeres and lamin A as modifiers of DNA damage response in cancer cells. Sci Rep. 2018;8:14827 pubmed 出版商
Zhai B, Steinø A, Bacha J, Brown D, Daugaard M. Dianhydrogalactitol induces replication-dependent DNA damage in tumor cells preferentially resolved by homologous recombination. Cell Death Dis. 2018;9:1016 pubmed 出版商
Sun J, Shi L, Kinomura A, Fukuto A, Horikoshi Y, Oma Y, et al. Distinct roles of ATM and ATR in the regulation of ARP8 phosphorylation to prevent chromosome translocations. elife. 2018;7: pubmed 出版商
Parisotto M, Grelet E, El Bizri R, Dai Y, Terzic J, Eckert D, et al. PTEN deletion in luminal cells of mature prostate induces replication stress and senescence in vivo. J Exp Med. 2018;215:1749-1763 pubmed 出版商
Vadnais C, Chen R, Fraszczak J, Yu Z, Boulais J, Pinder J, et al. GFI1 facilitates efficient DNA repair by regulating PRMT1 dependent methylation of MRE11 and 53BP1. Nat Commun. 2018;9:1418 pubmed 出版商
Göder A, Emmerich C, Nikolova T, Kiweler N, Schreiber M, Kühl T, et al. HDAC1 and HDAC2 integrate checkpoint kinase phosphorylation and cell fate through the phosphatase-2A subunit PR130. Nat Commun. 2018;9:764 pubmed 出版商
Chen L, Chen J, Huang Y, Gu Y, Qiu J, Qian H, et al. The Augmented R-Loop Is a Unifying Mechanism for Myelodysplastic Syndromes Induced by High-Risk Splicing Factor Mutations. Mol Cell. 2018;69:412-425.e6 pubmed 出版商
Michelini F, Pitchiaya S, Vitelli V, Sharma S, Gioia U, Pessina F, et al. Damage-induced lncRNAs control the DNA damage response through interaction with DDRNAs at individual double-strand breaks. Nat Cell Biol. 2017;19:1400-1411 pubmed 出版商
Chen Z, Cao Z, Zhang W, Gu M, Zhou Z, Li B, et al. LRRK2 interacts with ATM and regulates Mdm2-p53 cell proliferation axis in response to genotoxic stress. Hum Mol Genet. 2017;26:4494-4505 pubmed 出版商
Feng W, Jasin M. BRCA2 suppresses replication stress-induced mitotic and G1 abnormalities through homologous recombination. Nat Commun. 2017;8:525 pubmed 出版商
Gursoy Yuzugullu O, Carman C, Serafim R, Myronakis M, Valente V, Price B. Epigenetic therapy with inhibitors of histone methylation suppresses DNA damage signaling and increases glioma cell radiosensitivity. Oncotarget. 2017;8:24518-24532 pubmed 出版商
Higo T, Naito A, Sumida T, Shibamoto M, Okada K, Nomura S, et al. DNA single-strand break-induced DNA damage response causes heart failure. Nat Commun. 2017;8:15104 pubmed 出版商
Valdez B, Li Y, Murray D, Liu Y, Nieto Y, Champlin R, et al. The PARP inhibitor olaparib enhances the cytotoxicity of combined gemcitabine, busulfan and melphalan in lymphoma cells. Leuk Lymphoma. 2017;58:2705-2716 pubmed 出版商
Ashraf R, Hamidullah -, Hasanain M, Pandey P, Maheshwari M, Singh L, et al. Coumarin-chalcone hybrid instigates DNA damage by minor groove binding and stabilizes p53 through post translational modifications. Sci Rep. 2017;7:45287 pubmed 出版商
Zheng M, Zhu Z, Zhao Y, Yao D, Wu M, Sun G. Oridonin promotes G2/M arrest in A549 cells by facilitating ATM activation. Mol Med Rep. 2017;15:375-379 pubmed 出版商
Inoue S, Li W, Tseng A, Beerman I, Elia A, Bendall S, et al. Mutant IDH1 Downregulates ATM and Alters DNA Repair and Sensitivity to DNA Damage Independent of TET2. Cancer Cell. 2016;30:337-348 pubmed 出版商
Stankovic Valentin N, Drzewicka K, König C, Schiebel E, Melchior F. Redox regulation of SUMO enzymes is required for ATM activity and survival in oxidative stress. EMBO J. 2016;35:1312-29 pubmed 出版商
Sifford J, Stahl J, Salinas E, Forrest J. Murine Gammaherpesvirus 68 LANA and SOX Homologs Counteract ATM-Driven p53 Activity during Lytic Viral Replication. J Virol. 2015;90:2571-85 pubmed 出版商
Wang ondu R, Teal S, Park R, Heston L, Delecluse H, Miller G. DNA Damage Signaling Is Induced in the Absence of Epstein-Barr Virus (EBV) Lytic DNA Replication and in Response to Expression of ZEBRA. PLoS ONE. 2015;10:e0126088 pubmed 出版商
Modzelewski A, Hilz S, Crate E, Schweidenback C, Fogarty E, Grenier J, et al. Dgcr8 and Dicer are essential for sex chromosome integrity during meiosis in males. J Cell Sci. 2015;128:2314-27 pubmed 出版商
Valdez B, Li Y, Murray D, Ji J, Liu Y, Popat U, et al. Comparison of the cytotoxicity of cladribine and clofarabine when combined with fludarabine and busulfan in AML cells: Enhancement of cytotoxicity with epigenetic modulators. Exp Hematol. 2015;43:448-61.e2 pubmed 出版商
Broering T, Alavattam K, Sadreyev R, Ichijima Y, Kato Y, Hasegawa K, et al. BRCA1 establishes DNA damage signaling and pericentric heterochromatin of the X chromosome in male meiosis. J Cell Biol. 2014;205:663-75 pubmed 出版商
Mikawa T, Maruyama T, Okamoto K, Nakagama H, Lleonart M, Tsusaka T, et al. Senescence-inducing stress promotes proteolysis of phosphoglycerate mutase via ubiquitin ligase Mdm2. J Cell Biol. 2014;204:729-45 pubmed 出版商
Lim H, Xie L, Zhang W, Li R, Chen Z, Wu G, et al. Ribosomal S6 Kinase 2 (RSK2) maintains genomic stability by activating the Atm/p53-dependent DNA damage pathway. PLoS ONE. 2013;8:e74334 pubmed 出版商
Floyd S, Pacold M, Huang Q, Clarke S, Lam F, Cannell I, et al. The bromodomain protein Brd4 insulates chromatin from DNA damage signalling. Nature. 2013;498:246-50 pubmed 出版商
Daugherity E, Balmus G, Al Saei A, Moore E, Abi Abdallah D, Rogers A, et al. The DNA damage checkpoint protein ATM promotes hepatocellular apoptosis and fibrosis in a mouse model of non-alcoholic fatty liver disease. Cell Cycle. 2012;11:1918-28 pubmed 出版商
Hayashi M, Cesare A, Fitzpatrick J, Lazzerini Denchi E, Karlseder J. A telomere-dependent DNA damage checkpoint induced by prolonged mitotic arrest. Nat Struct Mol Biol. 2012;19:387-94 pubmed 出版商
Vadnais C, Davoudi S, Afshin M, Harada R, Dudley R, Clermont P, et al. CUX1 transcription factor is required for optimal ATM/ATR-mediated responses to DNA damage. Nucleic Acids Res. 2012;40:4483-95 pubmed 出版商
Vogel R, Seyffert M, Strasser R, de Oliveira A, Dresch C, Glauser D, et al. Adeno-associated virus type 2 modulates the host DNA damage response induced by herpes simplex virus 1 during coinfection. J Virol. 2012;86:143-55 pubmed 出版商
Sugimoto N, Yugawa T, Iizuka M, Kiyono T, Fujita M. Chromatin remodeler sucrose nonfermenting 2 homolog (SNF2H) is recruited onto DNA replication origins through interaction with Cdc10 protein-dependent transcript 1 (Cdt1) and promotes pre-replication complex formation. J Biol Chem. 2011;286:39200-10 pubmed 出版商
Zhang W, Peng G, Lin S, Zhang P. DNA damage response is suppressed by the high cyclin-dependent kinase 1 activity in mitotic mammalian cells. J Biol Chem. 2011;286:35899-905 pubmed 出版商
Fradet Turcotte A, Bergeron Labrecque F, Moody C, Lehoux M, Laimins L, Archambault J. Nuclear accumulation of the papillomavirus E1 helicase blocks S-phase progression and triggers an ATM-dependent DNA damage response. J Virol. 2011;85:8996-9012 pubmed 出版商
Ling S, Lin W. EDD inhibits ATM-mediated phosphorylation of p53. J Biol Chem. 2011;286:14972-82 pubmed 出版商
Al Rashid S, Harding S, Law C, Coackley C, Bristow R. Protein-protein interactions occur between p53 phosphoforms and ATM and 53BP1 at sites of exogenous DNA damage. Radiat Res. 2011;175:588-98 pubmed 出版商
Suzuki K, Yamauchi M, Oka Y, Suzuki M, Yamashita S. Creating localized DNA double-strand breaks with microirradiation. Nat Protoc. 2011;6:134-9 pubmed 出版商
Jurado S, Smyth I, van Denderen B, Tenis N, Hammet A, Hewitt K, et al. Dual functions of ASCIZ in the DNA base damage response and pulmonary organogenesis. PLoS Genet. 2010;6:e1001170 pubmed 出版商
Adeyemi R, Landry S, Davis M, Weitzman M, Pintel D. Parvovirus minute virus of mice induces a DNA damage response that facilitates viral replication. PLoS Pathog. 2010;6:e1001141 pubmed 出版商
Hurov K, Cotta Ramusino C, Elledge S. A genetic screen identifies the Triple T complex required for DNA damage signaling and ATM and ATR stability. Genes Dev. 2010;24:1939-50 pubmed 出版商
Larsen D, Poinsignon C, Gudjonsson T, Dinant C, Payne M, Hari F, et al. The chromatin-remodeling factor CHD4 coordinates signaling and repair after DNA damage. J Cell Biol. 2010;190:731-40 pubmed 出版商
Sato K, Nishikino M, Okano Y, Ohshima S, Hasegawa N, Ishino M, et al. ?-H2AX and phosphorylated ATM focus formation in cancer cells after laser plasma X irradiation. Radiat Res. 2010;174:436-45 pubmed 出版商
Boichuk S, Hu L, Hein J, Gjoerup O. Multiple DNA damage signaling and repair pathways deregulated by simian virus 40 large T antigen. J Virol. 2010;84:8007-20 pubmed 出版商
Efeyan A, Murga M, Martinez Pastor B, Ortega Molina A, Soria R, Collado M, et al. Limited role of murine ATM in oncogene-induced senescence and p53-dependent tumor suppression. PLoS ONE. 2009;4:e5475 pubmed 出版商
Sugimoto N, Yoshida K, Tatsumi Y, Yugawa T, Narisawa Saito M, Waga S, et al. Redundant and differential regulation of multiple licensing factors ensures prevention of re-replication in normal human cells. J Cell Sci. 2009;122:1184-91 pubmed 出版商
Sugimoto N, Kitabayashi I, Osano S, Tatsumi Y, Yugawa T, Narisawa Saito M, et al. Identification of novel human Cdt1-binding proteins by a proteomics approach: proteolytic regulation by APC/CCdh1. Mol Biol Cell. 2008;19:1007-21 pubmed
Jacquemont C, Taniguchi T. Proteasome function is required for DNA damage response and fanconi anemia pathway activation. Cancer Res. 2007;67:7395-405 pubmed
Ramirez T, Stopper H, Hock R, Herrera L. Prevention of aneuploidy by S-adenosyl-methionine in human cells treated with sodium arsenite. Mutat Res. 2007;617:16-22 pubmed
Bartkova J, Bakkenist C, Rajpert De Meyts E, Skakkebaek N, Sehested M, Lukas J, et al. ATM activation in normal human tissues and testicular cancer. Cell Cycle. 2005;4:838-45 pubmed
Bartkova J, Horejsi Z, Koed K, Krämer A, Tort F, Zieger K, et al. DNA damage response as a candidate anti-cancer barrier in early human tumorigenesis. Nature. 2005;434:864-70 pubmed
Falck J, Coates J, Jackson S. Conserved modes of recruitment of ATM, ATR and DNA-PKcs to sites of DNA damage. Nature. 2005;434:605-11 pubmed
Kitagawa R, Bakkenist C, McKinnon P, Kastan M. Phosphorylation of SMC1 is a critical downstream event in the ATM-NBS1-BRCA1 pathway. Genes Dev. 2004;18:1423-38 pubmed
Bakkenist C, Kastan M. DNA damage activates ATM through intermolecular autophosphorylation and dimer dissociation. Nature. 2003;421:499-506 pubmed
产品信息
目录号 :
200-301-400
名称 :
抗毛细血管扩张性共济失调症突变蛋白蛋白激酶pS1981(小鼠)单克隆抗体-200-301
显示名称 :
毛细血管扩张性共济失调症突变蛋白磷酸化S1981抗体
应用注释 :
Protein A Purified Mab anti-ATM has been tested by ELISA, Flow Cytometry, IF, and western blotting against both the native and recombinant forms of the protein. The antibody immunoprecipitates ATM from irradiated human and transfected mouse cells.  By immunofluorescence, foci are detected in irradiated human and mouse fibroblasts.  This antibody is not recommended for immunohistochemistry.  Instead, for IHC, use the clone 7C10D8 (p/n 200-301-500).
缓冲液 :
0.02 M钾磷酸盐, 0.15 M氯化钠, pH值7.2
克隆性 :
单克隆
克隆编号 :
10H11.E12
浓度值 :
1.0毫克/毫升
浓度定义 :
by UV absorbance at 280 nm
共轭 :
(None)
规格 :
100
默认单元 :
微克
ELISA稀释浓度 :
1:20, 000 - 1:100, 000
流式细胞仪稀释浓度 :
5微克/毫升
免疫组化稀释度 :
not recommended
免疫荧光显微镜稀释浓度 :
1:100 - 1:500
免疫沉淀稀释度 :
User Optimized
免疫印迹稀释浓度 :
1:200 - 1:2, 000
有效期 :
Expiration date is one (1) year from date of opening.
格式 :
IgG1
基因 :
毛细血管扩张性共济失调症突变蛋白
宿主动物 :
小鼠
一般免责声明 :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
免疫原 :
Anti-ATM phospho S1981 Antibody was produced from a synthetic peptide S-L-A-F-E-E-G-Sp-Q-S-T-T-I-S-S corresponding to aa 1974-1988 of human ATM.
其他性能数据 :
iFISH at 7.5 μg/ml
包装类型 :
干冰
物理状态 :
Liquid (sterile filtered)
防腐剂 :
0.01%(w/v)叠氮化钠
纯度和特异性 :
Anti-ATM phospho S1981 Monoclonal Antibody is directed against human ATM and is useful in determining its presence in various assays. This monoclonal anti-ATM antibody recognizes the phosphorylated epitope in native and over-expressed proteins found in various tissues and extracts.   By ELISA reactivity against SLAFEEGSpQSTTISS at a 1:1600 dilution shows an absorbance >3.000; whereas reactivity against SLAFEEGSQSTTISS shows and absorbance of 0.145.  Reactivity is observed against human ATM.  Cross reactivity with ATM from other mammalian sources has not been tested. The immunogen has 91% sequence homology with mouse ATM.
物种活性 :
H. sapiens (Human); Mus musculus (Mouse); Rattus (Rat)
储存 :
Store Anti-ATM phospho S1981 Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
子种类 :
IgG1 kappa
同义词 :
小鼠抗毛细血管扩张性共济失调症突变蛋白抗体, 小鼠抗ATMpS1981抗体, 小鼠抗毛细血管扩张性共济失调症突变蛋白pS1981抗体, DKFZp781A0353抗体, 人类磷脂酰肌醇类3激酶同系物抗体, MGC74674抗体, 丝氨酸蛋白激酶毛细血管扩张性共济失调症突变蛋白抗体, T细胞前淋巴细胞性白血病抗体
靶物种 :
人类
背景 :
Anti ATM pS1981 Antibody is a phospho site specific antibody and recognizes the product of the ATM gene that is mutated in the hereditary disease ataxia-telangiectasia. ATM codes for a protein kinase that acts as a master regulator of cellular responses to DNA double-strand breaks. ATM is normally inactive and the question of how it is activated in the event of DNA damage (due to ionizing radiation for instance) is central to understanding its function. ATM protein is now shown to be present in undamaged cells as an inactive dimer. Low doses of ionizing radiation, which induce only a few DNA breaks, activate at least half of the total ATM protein present, possibly in response to changes in chromatin structure.  The ATM gene encodes a 370-kDa protein that belongs to the phosphoinositide 3-kinase (PI(3)K) superfamily, but which phosphorylates proteins rather than lipids. The 350-amino-acid kinase domain at the carboxy terminus of this large protein is the only segment of ATM with an assigned function. Exposure of cells to IR triggers ATM kinase activity, and this function is required for arrests in G1, S and G2 phases of the cell cycle. Several substrates of the ATM kinase participate in these IR-induced cell-cycle arrests. These include p53, Mdm2 and Chk2 in the G1 checkpoint; Nbs1, Brca1, FancD2 and SMC1 in the transient IR-induced S-phase arrest; and Brca1 and hRad17 in the G2/M checkpoint. Ideal for Cancer, Cell Signaling, Chromatin, Neuroscience and Signal Transduction research.
免疫原类型 :
多肽
低内毒素 :
No
翻译后的修饰作用 :
磷酸化
样品尺寸 :
No
应用文本 :
酶联免疫吸附测定, 流式细胞仪, 免疫组化, IF显微镜, 免疫沉淀, 免疫印迹
其他 :
User Optimized
类别 :
一抗
共轭名称 :
未共轭
2022价格 :
499.00美元
UniProt数据库 :
Q13315
NCBI :
Q13315.3
最初图像名称 :
抗毛细血管扩张性共济失调症突变蛋白单克隆抗体-免疫荧光显微镜
更多信息或购买 :
公司信息
Rockland Immunochemicals
321 Jones Blvd
Pottstown, PA 19464
orders@rockland-inc.com
https://rockland-inc.com
800-656-7625
公司总部: 美国
Rockland Immunochemicals, Inc.位于宾夕法尼亚州的费城西部,为基础研究、应用研究及临床研究提供磷酸化位点特异性抗体及抗体相关研究工具。公司的技术平台是由一批经验丰富的科学家建立,利用In vitro及In vivo方法制备并纯化单抗及多抗,这些抗体应用于免疫印迹、ELISA、免疫组化、荧光检测、高通量筛选及诊断试剂盒生产等。常用一抗主要应用于以下研究:Akt通路、载脂蛋白、细胞凋亡/细胞周期、细胞因子、细胞信号、酶类、胞外基质、转录因子及泛素相关领域。