抗体

AKT抗体

200-301-401

100微克

单克隆

小鼠

未共轭

14E5.16C8.25F6

人类, 小鼠, 大鼠, 黑猩猩

免疫印迹, 酶联免疫吸附测定, 免疫组化, 流式细胞仪

抗AKT(小鼠)单克隆抗体-200-301

AKT抗体

Mouse Anti-AKT Antibody is suitable for ELISA, immunohistochemistry, immunoprecipitation and western blotting. Expect a band approximately 54 - 56 kDa in size corresponding to AKT protein by western blotting in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user. For immunohistochemistry we recommend the use of fresh frozen tissues. Attempts at staining paraffin-embedded formalin fixed tissues were negative. No pre-treatment of sample is required.

0.02 M钾磷酸盐, 0.15 M氯化钠, pH值7.2

单克隆

14E5.16C8.25F6

1.0毫克/毫升

by UV absorbance at 280 nm

(None)

100微克

微克

1:20,000

User Optimized

20微克/毫升

1:500 - 1:3, 000

Expiration date is one (1) year from date of opening.

IgG

AKT1, AKT2, AKT3

小鼠

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Anti-AKT Antibody was produced by repeated immunizations in mice with a synthetic peptide corresponding to residues internal to the human AKT1 protein.

干冰

Liquid (sterile filtered)

0.01%(w/v)叠氮化钠

Anti-AKT Antibody was purified by Protein A chromatography. This antibody is specific for human and mouse AKT protein.  A BLAST analysis was used to suggest cross-reactivity with AKT1, AKT2, and AKT3 from human, mouse, rat and chimpanzee sources based on 100% homology with the immunizing sequence.  Cross-reactivity with AKT1, 2, 3 was determined with Western Blot. Cross reactivity of AKT from other sources has not been determined.

人类, 小鼠, 大鼠, 黑猩猩

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

mouse anti-AKT Antibody, RAC-PK-alpha, Protein kinase B, PKB, C-AKT, RAC-alpha serine/threonine-protein kinase, Proto-oncogene c-Akt, AKT1, AKT 1, AKT-1

人类

AKT is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308. AKT is a cytoplasmic protein also known as AKT1, Protein Kinase B (PKB) and rac (related to A and C kinases). AKT is a key regulator of many signal transduction pathways. AKT exhibits tight control over cell proliferation and cell viability. Overexpression or inappropriate activation of AKT is noted in many types of cancer. AKT mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. AKT has two main roles: (i) inhibition of apoptosis; (ii) promotion of proliferation. Anti-AKT Antibody is ideal for investigators involved in Cell Signaling, Neuroscience, Signal Transduction research.

多肽

No

No

内部

酶联免疫吸附测定, 流式细胞仪, 免疫组化, 免疫印迹

User Optimized

一抗

未共轭

P31749

62241011

Western Blot of Mouse anti-AKT antibody