抗体

毛细血管扩张性共济失调症突变蛋白磷酸化S1981抗体

200-301-500

100微克

单克隆

小鼠

未共轭

7C10D8

人类, 小鼠

免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片

抗毛细血管扩张性共济失调症突变蛋白蛋白激酶pS1981(小鼠)单克隆抗体-200-301

毛细血管扩张性共济失调症突变蛋白磷酸化S1981抗体

This Anti-ATM Protein Kinase pS1981 (MOUSE) Monoclonal Antibody clone has been optimized for IHC, but may also be used for western blotting, flow cytometry, immunoprecipitation, or immunofluorescence microscopy. Indirect immunoperoxidase staining on formaldehyde-fixed, de-paraffinized tissue sections and/or formalin-fixed cultured cells are recommended when using this antibody as described in Bartkova et al 2005.  Product p/n 200-301-400 produced from clone 10H11.E12 has been optimized for WB, IP and IF.

0.02 M钾磷酸盐, 0.15 M氯化钠, pH值7.2

单克隆

7C10D8

1.0毫克/毫升

by UV absorbance at 280 nm

(None)

100微克

微克

1:10, 000 - 1:50, 000

User Optimized

1:100 - 1:500

1:500- 1:2, 000

Expiration date is one (1) year from date of opening.

IgG2a

毛细血管扩张性共济失调症突变蛋白

小鼠

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

This antibody was produced from a synthetic peptide S-L-A-F-E-E-G-Sp-Q-S-T-T-I-S-S corresponding to aa 1974-1988 of human ATM.

干冰

Liquid (sterile filtered)

0.01%(w/v)叠氮化钠

This Protein A Purified Mab antibody is directed against human ATM and is useful in determining its presence by immunohistochemistry. This monoclonal anti-ATM antibody recognizes the phosphorylated form of the protein in native and over-expressed proteins found in various tissues and extracts.  Reactivity is observed against human and mouse ATM.  Cross reactivity with ATM from other mammalian sources has not been tested.

人类, 小鼠

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

IgG2a

小鼠抗毛细血管扩张性共济失调症突变蛋白抗体, 小鼠抗ATMpS1981抗体, 小鼠抗毛细血管扩张性共济失调症突变蛋白pS1981抗体, DKFZp781A0353抗体, 人类磷脂酰肌醇类3激酶同系物抗体, MGC74674抗体, 丝氨酸蛋白激酶毛细血管扩张性共济失调症突变蛋白抗体, T细胞前淋巴细胞性白血病抗体

人类

Anti ATM pS1981 Antibody recognizes the product of the ATM gene that is mutated in the hereditary disease ataxia-telangiectasia. ATM codes for a protein kinase that acts as a master regulator of cellular responses to DNA double-strand breaks. ATM is normally inactive and the question of how it is activated in the event of DNA damage (due to ionizing radiation for instance) is central to understanding its function. ATM protein is now shown to be present in undamaged cells as an inactive dimer. Low doses of ionizing radiation, which induce only a few DNA breaks, activate at least half of the total ATM protein present, possibly in response to changes in chromatin structure.  The ATM gene encodes a 370-kDa protein that belongs to the phosphoinositide 3-kinase (PI(3)K) superfamily, but which phosphorylates proteins rather than lipids. The 350-amino-acid kinase domain at the carboxy terminus of this large protein is the only segment of ATM with an assigned function. Exposure of cells to IR triggers ATM kinase activity, and this function is required for arrests in G1, S and G2 phases of the cell cycle. Several substrates of the ATM kinase participate in these IR-induced cell-cycle arrests. These include p53, Mdm2 and Chk2 in the G1 checkpoint; Nbs1, Brca1, FancD2 and SMC1 in the transient IR-induced S-phase arrest; and Brca1 and hRad17 in the G2/M checkpoint. See Bakkenist, C. J. & Kastan, M. B. Nature 421, 499-506 (2003) for a complete presentation of this antibody's specificity and utility.

多肽

No

磷酸化

No

酶联免疫吸附测定, 流式细胞仪, 免疫组化, 免疫印迹

User Optimized

一抗

未共轭

Q13315

Q13315.3

抗毛细血管扩张性共济失调症突变蛋白单克隆抗体-免疫组化