抗体

α微管蛋白抗体

200-301-880

100微克

单克隆

小鼠

未共轭

17H11.F10

免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫细胞化学

抗甲-微管蛋白(小鼠)单克隆抗体-200-301

α微管蛋白抗体

Anti-Tubulin Antibody has been tested for use in ELISA, immunohistochemistry, immunofluorescence microscopy and western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band at ~50 kDa in size corresponding to alpha tubulin by western blotting in most cell lysates or extracts.

0.02 M钾磷酸盐, 0.15 M氯化钠, pH值7.2

单克隆

17H11.F10

1.00毫克/毫升

by UV absorbance at 280 nm

(None)

100微克

微克

1:300,000

2.5微克/毫升

0.1微克/毫升

1:1,000

Expiration date is one (1) year from date of opening.

IgG1

TUBA1B

小鼠

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Anti-Tubulin Loading Control Antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues near the C terminal end of human alpha tubulin protein.

干冰

Liquid (sterile filtered)

0.01%(w/v)叠氮化钠

Anti-Tubulin Loading Control Antibody was purified Protein A chromatography. This Loading Control antibody is directed against alpha tubulin.  A BLAST analysis was used to suggest antibody reactivity with alpha tubulin from a wide range of organisms, including avian, mammalian aquatic, parasitic and alga sources based on 100% homology for the immunogen sequence. Cross reactivity will occur with all isoforms of alpha tubulin. Such broad reactivity makes this antibody useful as an excellent loading control.

人类

Store Anti-Tubulin Antibody at -20° C prior to opening. Aliquot Loading Control Antibody and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge Tubliin Loading Control Antibody if not completely clear after standing at room temperature. This Anti-Tubulin Loading Control Antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

IgG1 kappa

mouse anti-Alpha-Tubulin Antibody, Tubulin alpha-1B chain, Tubulin alpha-ubiquitous chain, Alpha-tubulin ubiquitous, Tubulin K-alpha-1, TUBA1B, tubulin loading control

人类

Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Tubulin itself is a globular protein consisting of two polypeptides (alpha and beta tubulin). Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22-nm diameter. Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton-associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation, which is characteristic of microtubules in neuronal cells and the mitotic spindle.  This antibody makes an excellent loading control.

No

No

C末端

酶联免疫吸附测定, 免疫组化, IF显微镜, 免疫印迹

User Optimized

一抗

未共轭

P68363

17986283

抗α微管蛋白单克隆抗体-免疫组化