产品简要
公司名称 :
Rockland Immunochemicals
产品类型 :
抗体
产品名称 :
α微管蛋白抗体
目录 :
200-301-880
规格 :
100微克
价格 :
298.00美元
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
17H11.F10
反应物种 :
人类
应用 :
免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫细胞化学
更多信息或购买 :
文章摘录数: 6
出版应用/物种/样本/稀释参考文献
  • 免疫细胞化学; 人类; 图 2
Reisen F, Sauty De Chalon A, Pfeifer M, Zhang X, Gabriel D, Selzer P. Linking phenotypes and modes of action through high-content screen fingerprints. Assay Drug Dev Technol. 2015;13:415-27 pubmed 出版商
  • 免疫印迹; 人类; 图 1
Laitem C, Zaborowska J, Isa N, Kufs J, Dienstbier M, Murphy S. CDK9 inhibitors define elongation checkpoints at both ends of RNA polymerase II-transcribed genes. Nat Struct Mol Biol. 2015;22:396-403 pubmed 出版商
Xie C, Lin X, Wu D, Tan Y, Cheng C, Zhang J. Cardiac glycoside bufalin blocks cancer cell growth by inhibition of Aurora A and Aurora B activation via PI3K-Akt pathway. Oncotarget. 2018;9:13783-13795 pubmed 出版商
Roy K, Jerman S, Jozsef L, McNamara T, Onyekaba G, Sun Z, et al. Palmitoylation of the ciliary GTPase ARL13b is necessary for its stability and its role in cilia formation. J Biol Chem. 2017;292:17703-17717 pubmed 出版商
Patel S, Blaauboer S, Tucker H, Mansouri S, Ruiz Moreno J, Hamann L, et al. The Common R71H-G230A-R293Q Human TMEM173 Is a Null Allele. J Immunol. 2017;198:776-787 pubmed 出版商
Chavez R, Coricor G, Perez J, Seo H, Serra R. SOX9 protein is stabilized by TGF-β and regulates PAPSS2 mRNA expression in chondrocytes. Osteoarthritis Cartilage. 2017;25:332-340 pubmed 出版商
产品信息
目录号 :
200-301-880
名称 :
抗甲-微管蛋白(小鼠)单克隆抗体-200-301
显示名称 :
α微管蛋白抗体
应用注释 :
Anti-Tubulin Antibody has been tested for use in ELISA, immunohistochemistry, immunofluorescence microscopy and western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band at ~50 kDa in size corresponding to alpha tubulin by western blotting in most cell lysates or extracts.
缓冲液 :
0.02 M钾磷酸盐, 0.15 M氯化钠, pH值7.2
克隆性 :
单克隆
克隆编号 :
17H11.F10
浓度值 :
1.00毫克/毫升
浓度定义 :
by UV absorbance at 280 nm
共轭 :
(None)
规格 :
100微克
默认单元 :
微克
ELISA稀释浓度 :
1:300,000
免疫组化稀释度 :
2.5微克/毫升
免疫荧光显微镜稀释浓度 :
0.1微克/毫升
免疫印迹稀释浓度 :
1:1,000
有效期 :
Expiration date is one (1) year from date of opening.
格式 :
IgG1
基因名称 :
TUBA1B
宿主动物 :
小鼠
一般免责声明 :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
免疫原 :
Anti-Tubulin Loading Control Antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues near the C terminal end of human alpha tubulin protein.
包装类型 :
干冰
物理状态 :
Liquid (sterile filtered)
防腐剂 :
0.01%(w/v)叠氮化钠
纯度和特异性 :
Anti-Tubulin Loading Control Antibody was purified Protein A chromatography. This Loading Control antibody is directed against alpha tubulin.  A BLAST analysis was used to suggest antibody reactivity with alpha tubulin from a wide range of organisms, including avian, mammalian aquatic, parasitic and alga sources based on 100% homology for the immunogen sequence. Cross reactivity will occur with all isoforms of alpha tubulin. Such broad reactivity makes this antibody useful as an excellent loading control.
物种活性 :
人类
储存 :
Store Anti-Tubulin Antibody at -20° C prior to opening. Aliquot Loading Control Antibody and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge Tubliin Loading Control Antibody if not completely clear after standing at room temperature. This Anti-Tubulin Loading Control Antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
子种类 :
IgG1 kappa
同义词 :
mouse anti-Alpha-Tubulin Antibody, Tubulin alpha-1B chain, Tubulin alpha-ubiquitous chain, Alpha-tubulin ubiquitous, Tubulin K-alpha-1, TUBA1B, tubulin loading control
靶物种 :
人类
背景 :
Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Tubulin itself is a globular protein consisting of two polypeptides (alpha and beta tubulin). Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22-nm diameter. Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton-associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation, which is characteristic of microtubules in neuronal cells and the mitotic spindle.  This antibody makes an excellent loading control.
低内毒素 :
No
样品尺寸 :
No
靶活性结构域 :
C末端
应用文本 :
酶联免疫吸附测定, 免疫组化, IF显微镜, 免疫印迹
其他 :
User Optimized
类别 :
一抗
共轭名称 :
未共轭
2020价格 :
298.00美元
UniProt数据库 :
P68363
NCBI :
17986283
最初图像名称 :
抗α微管蛋白单克隆抗体-免疫组化
更多信息或购买 :
公司信息
Rockland Immunochemicals
321 Jones Blvd
Pottstown, PA 19464
orders@rockland-inc.com
https://rockland-inc.com
800-656-7625
公司总部: 美国
Rockland Immunochemicals, Inc.位于宾夕法尼亚州的费城西部,为基础研究、应用研究及临床研究提供磷酸化位点特异性抗体及抗体相关研究工具。公司的技术平台是由一批经验丰富的科学家建立,利用In vitro及In vivo方法制备并纯化单抗及多抗,这些抗体应用于免疫印迹、ELISA、免疫组化、荧光检测、高通量筛选及诊断试剂盒生产等。常用一抗主要应用于以下研究:Akt通路、载脂蛋白、细胞凋亡/细胞周期、细胞因子、细胞信号、酶类、胞外基质、转录因子及泛素相关领域。