产品简要
公司名称 :
Rockland Immunochemicals
产品类型 :
抗体
产品名称 :
磷酸甘油醛脱氢酶抗体
目录 :
200-301-A33
规格 :
100微克
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
33F2.C8.G6.E3
反应物种 :
domestic rabbit
应用 :
免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫沉淀
更多信息或购买 :
产品信息
目录号 :
200-301-A33
名称 :
抗甘油醛3脱氢酶(磷酸甘油醛脱氢酶)(小鼠)单克隆抗体-200-301
显示名称 :
磷酸甘油醛脱氢酶抗体
应用注释 :
Anti-GAPDH (Mouse) is suitable for use in Western Blotting. Specific conditions should be optimized by the end user. Expect a band of ~38kDa in size corresponding to Glyceraldehyde 3-Phosphate Dehydrogenase protein by Western blot in the appropriate cell lysate or extract.
缓冲液 :
0.1 M三羟甲基氨基甲烷氯化物, 0.5 M氯化钠, pH值8.0
克隆性 :
单克隆
克隆编号 :
33F2.C8.G6.E3
浓度值 :
1.0毫克/毫升
浓度定义 :
by UV absorbance at 280 nm
共轭 :
(None)
规格 :
100微克
默认单元 :
微克
ELISA稀释浓度 :
User Optimized
免疫组化稀释度 :
User Optimized
免疫荧光显微镜稀释浓度 :
1:100
免疫沉淀稀释度 :
User Optimized
免疫印迹稀释浓度 :
1:1,000
有效期 :
Expiration date is one (1) year from date of opening.
格式 :
IgM
基因名称 :
磷酸甘油醛脱氢酶
宿主动物 :
小鼠
一般免责声明 :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
免疫原 :
Anti-GAPDH Monoclonal Antibody was produced by repeated immunizations in mice with rabbit GAPDH protein.
包装类型 :
干冰
物理状态 :
Liquid (sterile filtered)
防腐剂 :
0.1%(w/v)叠氮化钠
纯度和特异性 :
Anti-GAPDH Monoclonal Antibody is directed against rabbit Glyceraldehyde-3-Phosphate Dehydrogenase. The antibody is a total IgM fraction prepared from ascites fluid by selective precipitation and tandem molecular sieve chromatography followed by extensive dialysis against the buffer stated above. Anti-GAPDH antibodies are expected to react with the following species based on 100% sequence homology: rabbit, human, and mouse. Reactivity against other sources is not known.
物种活性 :
兔, 人类, 小鼠
储存 :
Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.
菌株 :
Mouse - Mixed
子种类 :
IgM
同义词 :
mouse anti-GAPDH antibody, Loading Control Antibody, G3PDH, GAPD, Glyceraldehyde-3-phosphate dehydrogenase, Peptidyl-cysteine S-nitrosylase GAPDH
靶物种 :
背景 :
GAPDH Loading Control Monoclonal Antibody detects Glyceraldehyde-3-Phosphate Dehydrogenase. GAPDH is a metabolic enzyme responsible for catalyzing one step in the glycolytic pathway, the reversible oxidative phosphorylation of glyceraldehyde 3-phosphate. Because GAPDH is a protein expressed in large amounts and which is required at all times for important "house keeping" functions, levels of GAPDH mRNA are often measured and used as standards in studies of mRNA expression. Increasingly, scientists are making use of specific antibodies to GAPDH in comparable studies of levels of protein expression. Apart from a role in glycolysis, GAPDH may have other roles such as in the activation of transcription. GAPDH is reported to bind to a variety of other proteins, including the amyloid precursor protein, mutations in which cause some forms of Alzheimer's disease, and the polyglutamine tracts of Huntingtin, the protein product aberrant forms of which are causative of Huntington's disease. Associations with actin and tubulin have also been reported. The protein may also have a role in the regulation of apoptosis, and interestingly migrates from the cytoplasm into the nucleus when cells become apoptotic. Anti-GAPDH antibody is widely used as a loading control for western blotting experiments, allowing comparison between the level of this protein and others in a cell or tissue and is ideal for Neuroscience, Cell Signaling and Cancer Research.
免疫原类型 :
天然蛋白
低内毒素 :
No
样品尺寸 :
No
应用文本 :
酶联免疫吸附测定, 免疫组化, IF显微镜, 免疫沉淀, 免疫印迹
类别 :
一抗
共轭名称 :
未共轭
UniProt数据库 :
P46406
NCBI :
NP_001075722.1
最初图像名称 :
Immunofluorescence Microscopy of Mouse Anti-GAPDH antibody
更多信息或购买 :
公司信息
Rockland Immunochemicals
321 Jones Blvd
Pottstown, PA 19464
orders@rockland-inc.com
https://www.rockland.com/
800-656-7625
公司总部: 美国
Rockland Immunochemicals, Inc.位于宾夕法尼亚州的费城西部,为基础研究、应用研究及临床研究提供磷酸化位点特异性抗体及抗体相关研究工具。公司的技术平台是由一批经验丰富的科学家建立,利用In vitro及In vivo方法制备并纯化单抗及多抗,这些抗体应用于免疫印迹、ELISA、免疫组化、荧光检测、高通量筛选及诊断试剂盒生产等。常用一抗主要应用于以下研究:Akt通路、载脂蛋白、细胞凋亡/细胞周期、细胞因子、细胞信号、酶类、胞外基质、转录因子及泛素相关领域。