抗体

Hsp70/Hsc70抗体

200-301-F64

200微克

单克隆

小鼠

未共轭

BB70

人类, 小鼠, 大鼠, 牛

免疫印迹, 免疫组化, 免疫沉淀

抗Hsp70/Hsc70(小鼠)单克隆抗体-200-301

Hsp70/Hsc70抗体

Anti-Hsp70/Hsc70 Antibody is suitable for WB, IP, and IHC. Expect a band approximately ~72 and ~73kDa proteins corresponding to the molecular mass of inducible Hsp and Hsc on SDS PAGE immunoblots. This monoclonal antibody reacts with chicken Hsp70/Hsp90 complex. Specific conditions for reactivity should be optimized by the end user.

0.02 M钾磷酸盐, 0.15 M氯化钠, pH值7.2

单克隆

BB70

1毫克/毫升

by UV absorbance at 280 nm

(None)

200微克

微克

User Optimized

User Optimized

1μg/毫升

Expiration date is one (1) year from date of opening.

IgG2a

HSPA1A

小鼠

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Hsp70/Hsc70 Antibody was produced in mice by repeated immunizations raised against chicken Hsp70/Hsp90 complex.

干冰

Liquid (sterile filtered)

Anti-Hsp70/Hsc70 Antibody was purified by Protein G chromatography. A BLAST analysis was used to suggest cross-reactivity with Hsp70/Hsc70 from Human, Mouse, Rat, Sheep, Dog, Beluga, Bovine, Fish, Guinea pig, Scallop pig, Hamster, Rabbit, Chicken, Xenopus, Drosophila, Yeast sources based on 100% homology with the immunizing sequence. Cross-reactivity with Hsp70/Hsc70 from other sources has not been determined. Heat Shock research.

Human, Mouse, Rat, Sheep, Dog, Beluga, Bovine, Fish, Guinea pig, Scallop pig, Hamster, Rabbit, Chicken, Xenopus, Drosophila, Yeast

50%(v/v)甘油

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

IgG2a

Hsp70 1, Hsp70 2, Hsp70.1, Hsp72, Hsp73, HSPA1, HSPA1A, HSPA1B, Heat shock 70 kDa protein 1A/1B, Heat shock 70 kDa protein 1/2, HSP70-1/HSP70-2

鸡

Hsp70 genes encode abundant heat-inducible 70-kDa hsps (hsp70s). In most eukaryotes hsp70 genes exist as part of a multigene family. They are found in most cellular compartments of eukaryotes including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 5O% identity. The N-terminal two thirds of hsp70s are more conserved than the C-terminal third. Hsp70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides. When hsc70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44 kDa which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the C-terminal half. The structure of this ATP binding domain displays multiple features of nucleotide binding proteins. All hsp70s, regardless of location, bind proteins, particularly unfolded ones. The molecular chaperones of the hsp70 family recognize and bind to nascent polypeptide chains as well as partially folded intermediates of proteins preventing their aggregation and misfolding. The binding of ATP triggers a critical conformational change leading to the release of the bound substrate protein. The universal ability of hsp70s to undergo cycles of binding to and release from hydrophobic stretches of partially unfolded proteins determines their role in a great variety of vital intracellular functions such as protein synthesis, protein folding and oligomerization and protein transport.

其他

No

No

免疫组化, 免疫沉淀, 免疫印迹

一抗

未共轭

P0DMV8

NP_005336.3

Hsp70/Hsc 70 Immunohistochemistry