产品简要
公司名称 :
Rockland Immunochemicals
产品类型 :
抗体
产品名称 :
Hsp90抗体
目录 :
200-301-F68
规格 :
100微克
价格 :
369.00美元
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
D7A
反应物种 :
人类, 小鼠, 大鼠, ,
应用 :
免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫沉淀
更多信息或购买 :
产品信息
目录号 :
200-301-F68
名称 :
抗Hsp90(小鼠)单克隆抗体-200-301
显示名称 :
Hsp90抗体
应用注释 :
Anti-Hsp90 Antibody is suitable for WB, IP, ELISA, and IHC. Expect a band approximately 90kD proteins corresponding to the molecular mass of Hsp90 on SDS Page immunoblots. Specific conditions for reactivity should be optimized by the end user.
缓冲液 :
0.02 M钾磷酸盐, 0.15 M氯化钠, pH值7.2
克隆性 :
单克隆
克隆编号 :
D7A
浓度值 :
1毫克/毫升
浓度定义 :
by UV absorbance at 280 nm
共轭 :
(None)
规格 :
100微克
默认单元 :
微克
ELISA稀释浓度 :
1:200
免疫组化稀释度 :
User Optimized
免疫沉淀稀释度 :
5µg/20微升
免疫印迹稀释浓度 :
1:500
有效期 :
Expiration date is one (1) year from date of opening.
格式 :
IgG
基因名称 :
HSP90AA1
宿主动物 :
小鼠
一般免责声明 :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
免疫原 :
Hsp90 Antibody was produced in mice by repeated immunizations raised against full length protein Hsp90 purified from chicken brain.
包装类型 :
干冰
物理状态 :
Liquid (sterile filtered)
防腐剂 :
0.09%(w/v)叠氮化钠
纯度和特异性 :
Anti-Hsp90 Antibody was purified by Protein G chromatography. A BLAST analysis was used to suggest cross-reactivity with Hsp90 from Bovine, Chicken, Human, Mouse, Porcine, Rat, and Rabbit based on 100% homology with the immunizing sequence. Hsp90α specific for human samples. Can isolate complexes of Hsp90, Src kinase and cec37. Cross-reactivity with Hsp90 from other sources has not been determined. Heat Shock research.
物种活性 :
牛, 鸡, 人类, 小鼠, 猪(猪), 大鼠, 兔
稳定剂 :
50%(v/v)甘油
储存 :
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
子种类 :
IgG
同义词 :
Hsp84, Hsp90, Hsp90乙, Hsp90B, HspC2, HSPCB, 热休克蛋白HSP 90甲, HSP90AA1, HSPCA
靶物种 :
背景 :
Hsp90 is a highly conserved and essential stress protein that is expressed in all eukaryotic cells. From a functional perspective, hsp90 participates in the folding, assembly, maturation, and stabilization of specific proteins as an integral component of a chaperone complex. Despite its label of being a heat-shock protein, hsp90 is one of the most highly expressed proteins in unstressed cells (1–2% of cytosolic protein). It carries out a number of housekeeping functions – including controlling the activity, turnover, and trafficking of a variety of proteins. Most of the hsp90- regulated proteins that have been discovered to date are involved in cell signaling. The number of proteins now know to interact with Hsp90 is about 100. Target proteins include the kinases v-Src, Wee1, and c-Raf, transcriptional regulators such as p53 and steroid receptors, and the polymerases of the hepatitis B virus and telomerase.5 When bound to ATP, Hsp90 interacts with co-chaperones Cdc37, p23, and an assortment of immunophilin-like proteins, forming a complex that stabilizes and protects target proteins from proteasomal degradation. In most cases, hsp90-interacting proteins have been shown to co-precipitate with hsp90 when carrying out immunoadsorption studies, and to exist in cytosolic heterocomplexes with it. In a number of cases, variations in hsp90 expression or hsp90 mutation has been shown to degrade signaling function via the protein or to impair a specific function of the protein (such as steroid binding, kinase activity) in vivo. Ansamycin antibiotics, such as geldanamycin and radicicol, inhibit hsp90 function.
免疫原类型 :
其他
低内毒素 :
No
样品尺寸 :
No
应用文本 :
酶联免疫吸附测定, 免疫组化, 免疫沉淀, 免疫印迹
类别 :
一抗
共轭名称 :
未共轭
2020价格 :
369.00美元
UniProt数据库 :
P11501
NCBI :
NP_001103255.1
最初图像名称 :
Hsp90免疫组化
更多信息或购买 :
公司信息
Rockland Immunochemicals
321 Jones Blvd
Pottstown, PA 19464
orders@rockland-inc.com
https://rockland-inc.com
800-656-7625
公司总部: 美国
Rockland Immunochemicals, Inc.位于宾夕法尼亚州的费城西部,为基础研究、应用研究及临床研究提供磷酸化位点特异性抗体及抗体相关研究工具。公司的技术平台是由一批经验丰富的科学家建立,利用In vitro及In vivo方法制备并纯化单抗及多抗,这些抗体应用于免疫印迹、ELISA、免疫组化、荧光检测、高通量筛选及诊断试剂盒生产等。常用一抗主要应用于以下研究:Akt通路、载脂蛋白、细胞凋亡/细胞周期、细胞因子、细胞信号、酶类、胞外基质、转录因子及泛素相关领域。