产品简要
公司名称 :
Rockland Immunochemicals
产品类型 :
抗体
产品名称 :
溴化去氧尿苷抗体
目录 :
200-301-H50
规格 :
100微克
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
29G6.E8
应用 :
免疫印迹, 酶联免疫吸附测定, 免疫组化, 流式细胞仪
更多信息或购买 :
文章摘录数: 2
参考文献
Silva C, Peyre E, Adhikari M, Tielens S, Tanco S, Van Damme P, et al. Cell-Intrinsic Control of Interneuron Migration Drives Cortical Morphogenesis. Cell. 2018;172:1063-1078.e19 pubmed 出版商
Wang Z, Shen W, Cheng F, Deng X, Engelhardt J, Yan Z, et al. Parvovirus Expresses a Small Noncoding RNA That Plays an Essential Role in Virus Replication. J Virol. 2017;91: pubmed 出版商
产品信息
目录号 :
200-301-H50
名称 :
抗溴化去氧尿苷(小鼠)单克隆抗体-200-301
显示名称 :
溴化去氧尿苷抗体
应用注释 :
Anti-BrdU Antibody has been tested as suitable for immunofluorescence and immunoblot assays. Antibody may be suitable for additional immunoassays including flow cytometry and immunohistochemistry. Specific conditions for reactivity should be optimized by the end user. Antibody will detect incorporated BrdU thymidine analog from replicated cells.
缓冲液 :
0.02 M钾磷酸盐, 0.15 M氯化钠, pH值7.2
克隆性 :
单克隆
克隆编号 :
29G6.E8
浓度值 :
1.0毫克/毫升
浓度定义 :
by UV absorbance at 280 nm
共轭 :
(None)
规格 :
100微克
默认单元 :
微克
ELISA稀释浓度 :
1:2000 - 1:10000
流式细胞仪稀释浓度 :
1:50-1:100
免疫组化稀释度 :
1:100-1:500
免疫荧光显微镜稀释浓度 :
1:500-1:3000
免疫印迹稀释浓度 :
1:2000 - 1:5000
有效期 :
Expiration date is one (1) year from date of opening.
格式 :
IgG
宿主动物 :
小鼠
一般免责声明 :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
免疫原 :
Anti-BrdU monoclonal antibody was produced in mice by repeated immunizations prepared via immunizations with BromodeoxyUridine-KLH followed by hybridoma development.
包装类型 :
干冰
物理状态 :
Liquid (sterile filtered)
防腐剂 :
0.01%(w/v)叠氮化钠
纯度和特异性 :
Anti-BrdU Monoclonal Antibody was purified from ascites fluid by Protein A chromatography. This antibody reacts strongly with BrdU. Cross-reactivity is observed with CldU and IdU.
物种活性 :
BrdU, IdU, CldU
储存 :
Store BrdU Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
子种类 :
IgG1 lambda
同义词 :
mouse anti-BrdU Antibody, Bromodeoxyuridine, 5-bromo-2'-deoxyuridine, BrdU
背景 :
Bromodeoxyuridine (5-bromo-2'-deoxyuridine, BrdU) is a synthetic thymidine nucleoside analog. BrdU is commonly used to allow the detection of growing or proliferating cells in living tissues. During the S-phase of cell division, DNA replication occurs, and BrdU can be incorporated into the newly synthesized DNA by substituting for naturally occurring thymidine. Antibodies specific for BrdU are subsequently used to detect the incorporated BrdU thymidine analog. This highlights cells that were actively replicating their DNA and is suggestive of actively growing cells. Antibody binding usually requires the DNA to be denatured, typically by exposing the cells to acid or heat.
免疫原类型 :
其他
低内毒素 :
No
样品尺寸 :
No
应用文本 :
酶联免疫吸附测定, 流式细胞仪, 免疫组化, IF显微镜, 免疫印迹
类别 :
一抗
共轭名称 :
未共轭
最初图像名称 :
免疫荧光显微镜-小鼠抗溴化去氧尿苷
更多信息或购买 :
公司信息
Rockland Immunochemicals
321 Jones Blvd
Pottstown, PA 19464
orders@rockland-inc.com
https://www.rockland.com/
800-656-7625
公司总部: 美国
Rockland Immunochemicals, Inc.位于宾夕法尼亚州的费城西部,为基础研究、应用研究及临床研究提供磷酸化位点特异性抗体及抗体相关研究工具。公司的技术平台是由一批经验丰富的科学家建立,利用In vitro及In vivo方法制备并纯化单抗及多抗,这些抗体应用于免疫印迹、ELISA、免疫组化、荧光检测、高通量筛选及诊断试剂盒生产等。常用一抗主要应用于以下研究:Akt通路、载脂蛋白、细胞凋亡/细胞周期、细胞因子、细胞信号、酶类、胞外基质、转录因子及泛素相关领域。