抗体

雄激素受体抗体

200-301-H54

100微克

单克隆

小鼠

未共轭

156C135.2

免疫印迹, 免疫组化

抗雄激素受体(小鼠)单克隆抗体-200-301

雄激素受体抗体

Anti-Androgen Receptor Antibody is suitable for use in IHC (p) and WB. Expect a band approximately 110kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

0.02 M钾磷酸盐, 0.15 M氯化钠, pH值7.2

单克隆

156C135.2

1.0毫克/毫升

by UV absorbance at 280 nm

(None)

100微克

微克

User Optimized

1-4微克/毫升

Expiration date is one (1) year from date of opening.

IgG1

AR

小鼠

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Androgen Receptor Antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to amino acids near the n-terminus of the Androgen Receptor.

干冰

液体

0.05%(w/v)叠氮化钠

Anti-Androgen Receptor Antibody was purified by Protein G chromatography. A BLAST analysis was used to suggest cross-reactivity with Anti-Androgen Receptor from human and New World Monkey on 100% homology with the immunizing sequence. Cross-reactivity with Anti-Androgen Receptor from other sources has not been determined.

人类

0.05% BSA

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

IgG1 kappa

Androgen receptor, Dihydrotestosterone receptor, Nuclear receptor subfamily 3 group C member 4, AR, DHTR, NR3C4

人类

Anti-Androgen Receptor Antibody detects human Androgen receptor. The androgen receptor (AR) is an ~110 kDa androgen-dependent transcription factor that is a member of the steroid/nuclear receptor gene superfamily. The AR signaling pathway plays a key role in development and function of male reproductive organs, including the prostate and epididymis. AR also plays a role in nonreproductive organs, such as muscle, hair follicles, and brain. Abnormalities in the AR signaling pathway have been linked to a number of diseases, including prostate cancer, Kennedy's disease and male infertility. The PI3K/Akt signaling pathway plays an important role in regulating AR activity through phosphorylation of AR at Ser213/210 and Ser791/790. Growth factors or cytokines may induce phosphorylation of AR through the PI3K/Akt pathway. IGF-1 activates the phosphatidylinositol 3-kinase(PI3K)/AKT pathway in LNCap at high passage number and increases phosphorylation of of AR at Ser213/210 (see western blot) and Ser791/790. The western blot results also show that inhibition of the PI3K/Akt pathway by LY294002 prior to incubation with IGF-1 suppressed AR phosphorylation at Ser213/210. Activation of the PI3K/AKt pathway is thought to have a survival role in prostate cancer by protecting cells from apoptosis. Anti-Androgen Receptor Antibody is ideal for investigators involved in neuroscience, cytokines and growth factors, and transcription factors.

多肽

No

No

N末端

免疫组化, 免疫印迹

一抗

未共轭

P10275

NP_000035.2

雄激素受体免疫印迹