产品简要
公司名称 :
Rockland Immunochemicals
产品类型 :
抗体
产品名称 :
CD3荧光素抗体
目录 :
200-302-N14
规格 :
500微升
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
异硫氰酸荧光素
克隆名称 :
UCHT1
反应物种 :
人类
应用 :
免疫印迹, 免疫组化, 免疫沉淀, 流式细胞仪
更多信息或购买 :
产品信息
目录号 :
200-302-N14
名称 :
抗CD3(小鼠)荧光素共轭标签单克隆抗体-200-302
显示名称 :
CD3荧光素抗体
应用注释 :
Anti-CD3 is useful for Immunoprecipitation, Immunohistochemistry, and Flow Cytometry. Researchers should determine optimal titers for applications that are not stated.
缓冲液 :
0.01 M钠磷酸盐, 0.15 M氯化钠, pH值7.2
克隆性 :
单克隆
克隆编号 :
UCHT1
浓度值 :
0.2毫克/毫升
浓度定义 :
Sufficient to run approximately 100 tests
共轭 :
异硫氰酸荧光素
规格 :
500微升
默认单元 :
微升
流式细胞仪稀释浓度 :
5µL/test/1x10e5 to 1x10e8 cells
荧光蛋白值 :
2-8
免疫组化稀释度 :
User Optimized
免疫沉淀稀释度 :
User Optimized
免疫印迹稀释浓度 :
User Optimized
有效期 :
Expiration date is six (6) months from date of opening.
格式 :
IgG1
基因名称 :
CD3E
宿主动物 :
小鼠
一般免责声明 :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
免疫原 :
Anti-CD3 Antibody (Monoclonal) was produced by repeated immunizations with CD3 antigen.
包装类型 :
Wet Ice
物理状态 :
Liquid (sterile filtered)
防腐剂 :
0.09%(w/v)叠氮化钠
纯度和特异性 :
Fluorescein conjugated CD3 Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against human CD3. Reactivity is observed against human and chimpanzee. Cross reactivity with CD3 from other sources has not been tested. Anti-CD3 is conjugated with FITC under optimal conditions and the solution is free of unconjugated FITC.
物种活性 :
人类
稳定剂 :
0.1% 明胶
储存 :
Store vial at 4° C prior to opening.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  DO NOT FREEZE. This product is light sensitive.
子种类 :
IgG1 kappa
同义词 :
T-cell surface glycoprotein CD3 epsilon chain, T-cell surface antigen T3/Leu-4 epsilon chain, CD3e
靶物种 :
人类
背景 :
The UCHT1 antibody is specific for human CD3e, also known as CD3 epsilon, a 20 kDa subunit of the T cell receptor complex, along with CD3 gamma and CD3 delta. These integral membrane protein chains assemble with additional chains of the T cell receptor (TCR), as well as CD3 zeta chain, to form the T cell receptor – CD3 complex. Together with co-receptors CD4 or CD8, the complex serves to recognize antigens bound to MHC molecules on antigen-presenting cells. These interactions promote T cell receptor signaling (T cell activation), inducing cell proliferation, differentiation, production of cytokines or activation-induced cell death. CD3 is differentially expressed during thymocyte-to-T cell development and on all mature T cells.The UCHT1 antibody is a widely used phenotypic marker for human T cells. In addition, binding/cross-linking of UCHT1 antibody to CD3e can induce cell activation. A recent publication of the crystal structure of a CD3e- antibody complex provides insight as to the action of commonly used agonist antibodies, as well as specific epitope-binding data for the human CD3 antibodies UCHT1 and OKT3. UCHT1 antibody reacts with both surface-expressed and intracellular CD3e protein, in contrast to an alternative human CD3 clone, HIT3a, which will stain only the extracellular (membrane-expressed) CD3e protein. Also, the UCHT1 antibody is reported to be cross-reactive with chimpanzee and has been used for phenotypic analysis of expression by flow cytometry; however the antibody is reported to be unsuitable for induction of T cell activation in this species.
低内毒素 :
No
样品尺寸 :
No
应用文本 :
流式细胞仪, 免疫组化, 免疫沉淀, 免疫印迹
类别 :
一抗
共轭名称 :
荧光素(异硫氰酸荧光素)
UniProt数据库 :
P07766
NCBI :
NP_000724.1
最初图像名称 :
流式细胞仪-小鼠抗CD3异硫氰酸荧光素
更多信息或购买 :
公司信息
Rockland Immunochemicals
321 Jones Blvd
Pottstown, PA 19464
orders@rockland-inc.com
https://www.rockland.com/
800-656-7625
公司总部: 美国
Rockland Immunochemicals, Inc.位于宾夕法尼亚州的费城西部,为基础研究、应用研究及临床研究提供磷酸化位点特异性抗体及抗体相关研究工具。公司的技术平台是由一批经验丰富的科学家建立,利用In vitro及In vivo方法制备并纯化单抗及多抗,这些抗体应用于免疫印迹、ELISA、免疫组化、荧光检测、高通量筛选及诊断试剂盒生产等。常用一抗主要应用于以下研究:Akt通路、载脂蛋白、细胞凋亡/细胞周期、细胞因子、细胞信号、酶类、胞外基质、转录因子及泛素相关领域。