产品简要
公司名称 :
Rockland Immunochemicals
产品类型 :
抗体
产品名称 :
CD27藻红蛋白抗体
目录 :
200-308-N65
规格 :
500微升
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
RPE
克隆名称 :
O323
反应物种 :
人类
应用 :
流式细胞仪
更多信息或购买 :
产品信息
目录号 :
200-308-N65
名称 :
抗CD27(小鼠)藻红蛋白共轭标签单克隆抗体-200-308
显示名称 :
CD27藻红蛋白抗体
应用注释 :
Anti-CD27 is useful for Flow Cytometry. Researchers should determine optimal titers for applications that are not stated.
缓冲液 :
0.01 M钠磷酸盐, 0.15 M氯化钠, pH值7.2
克隆性 :
单克隆
克隆编号 :
O323
浓度值 :
0.05毫克/毫升
浓度定义 :
Sufficient to run approximately 100 tests
共轭 :
RPE
规格 :
500微升
默认单元 :
微升
流式细胞仪稀释浓度 :
5µL/test/1x10e5 to 1x10e8 cells
荧光蛋白值 :
1-2
有效期 :
Expiration date is six (6) months from date of opening.
格式 :
IgG1
基因名称 :
CD27
宿主动物 :
小鼠
一般免责声明 :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
免疫原 :
Anti-CD27 Antibody (Monoclonal) was produced by repeated immunizations with CD27 antigen.
包装类型 :
Wet Ice
物理状态 :
Liquid (sterile filtered)
防腐剂 :
0.09%(w/v)叠氮化钠
纯度和特异性 :
Phycoerythrin conjugated CD27 Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against human CD27. Reactivity is observed against human CD27, Baboon, Cynomolgus, and Rhesus. Cross reactivity with CD27 from other sources has not been tested. Anti-CD27 is conjugated with PE under optimal conditions and the solution is free of unconjugated PE.
物种活性 :
人类
稳定剂 :
0.1% 明胶
储存 :
Store vial at 4° C prior to opening. Dilute only prior to immediate use. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis. Store reagent in the dark. DO NOT FREEZE.
子种类 :
IgG1 kappa
同义词 :
CD27 antigen, CD27L receptor, T-cell activation antigen CD27, T14, Tumor necrosis factor receptor superfamily member 7, CD27, TNFRSF7
靶物种 :
人类
背景 :
CD27 antibody reacts with human CD27 (TNFRSF7), a cell surface homodimer of 55 kDa subunits, which provides co-stimulatory signaling in support of the T cell (TCR) and B cell (BCR) receptors. By comparison with CD28, whose TCR co-stimulatory signal can trigger cell proliferation, CD27 signaling appears to promote cell survival and differentiation to effector / memory stages. Also in contrast with CD28, the CD27 receptor may be shed following interaction with its ligand CD70, which is typically expressed on activated dendritic cells, T cells and B cells. With respect to B cells, CD27 is considered to be a phenotypic marker for memory B cells. CD27 has been included within a group of phenotypic markers for identifying human B regulatory cells (Bregs), a cell type proposed to regulate CD4+ T cell proliferation and Foxp3 / CTLA-4 expression in Treg cells. The CD27 antibody may be used for analysis of CD27 expression on peripheral T cells, and is frequently used in combination with antibodies for IgD and IgM to distinguish naïve vs. memory B cell populations. For identification of Breg cells, this antibody has been used in combination with antibodies for CD25, CD27, CD1d, IL-10 and TGF-beta.
低内毒素 :
No
样品尺寸 :
No
应用文本 :
流式细胞仪
类别 :
一抗
共轭名称 :
R藻红蛋白(RPE)
UniProt数据库 :
P26842
NCBI :
NP_001233.1
最初图像名称 :
流式细胞仪-小鼠抗CD27 PE
更多信息或购买 :
公司信息
Rockland Immunochemicals
321 Jones Blvd
Pottstown, PA 19464
orders@rockland-inc.com
https://www.rockland.com/
800-656-7625
公司总部: 美国
Rockland Immunochemicals, Inc.位于宾夕法尼亚州的费城西部,为基础研究、应用研究及临床研究提供磷酸化位点特异性抗体及抗体相关研究工具。公司的技术平台是由一批经验丰富的科学家建立,利用In vitro及In vivo方法制备并纯化单抗及多抗,这些抗体应用于免疫印迹、ELISA、免疫组化、荧光检测、高通量筛选及诊断试剂盒生产等。常用一抗主要应用于以下研究:Akt通路、载脂蛋白、细胞凋亡/细胞周期、细胞因子、细胞信号、酶类、胞外基质、转录因子及泛素相关领域。