产品简要
公司名称 :
Rockland Immunochemicals
产品类型 :
抗体
产品名称 :
AKT磷酸化S473 DyLight系列染料549共轭标签抗体
目录 :
200-342-268
规格 :
100微克
价格 :
369.00美元
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
DyLight549
克隆名称 :
17F6.B11
反应物种 :
人类, 小鼠, 大鼠
应用 :
免疫印迹, 酶联免疫吸附测定, 流式细胞仪
更多信息或购买 :
产品信息
目录号 :
200-342-268
名称 :
Anti-AKT pS473 (MOUSE) Monoclonal Antibody DyLight™ 549 Conjugated - 200-342-268
显示名称 :
AKT磷酸化S473 DyLight系列染料549共轭标签抗体
应用注释 :
This monoclonal antibody is suitable for immunofluorescence microscopy, FLISA, flow cytometry, and western blotting. Expect a band approximately 56 kDa in size corresponding to phosphorylated AKT protein by western blotting in the appropriate cell lysate or extract. This phospho-specific monoclonal antibody reacts with human and mouse AKT pS473 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide. Specific conditions for reactivity should be optimized by the end user.
缓冲液 :
0.02 M钾磷酸盐, 0.15 M氯化钠, pH值7.2
克隆性 :
单克隆
克隆编号 :
17F6.B11
浓度值 :
1.0毫克/毫升
浓度定义 :
by UV absorbance at 280 nm
共轭 :
DL549
规格 :
100微克
默认单元 :
微克
ELISA稀释浓度 :
1:4, 000 - 1:20, 000
FLISA稀释浓度 :
>1:20,000
流式细胞仪稀释浓度 :
User Optimized
荧光蛋白值 :
4.0
免疫荧光显微镜稀释浓度 :
>1:5,000
免疫印迹稀释浓度 :
>1:10,000
有效期 :
Expiration date is one (1) year from date of opening.
格式 :
IgG1
基因名称 :
AKT1
宿主动物 :
小鼠
一般免责声明 :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
免疫原 :
This monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding S473 of human AKT1 protein.
包装类型 :
Ambient
物理状态 :
冻干
防腐剂 :
0.01%(w/v)叠氮化钠
纯度和特异性 :
This product was purified from concentrated tissue culture supernate by Protein A chromatography. This antibody is specific for human and mouse AKT protein phosphorylated at S473. A BLAST analysis was used to suggest cross-reactivity with AKT pS473 from human, mouse, rat and chimpanzee sources based on 100% homology with the immunizing sequence. Cross-reactivity with AKT from other sources has not been determined. Cross-reactivity with AKT2 and AKT3 has not been determined.
重组缓冲液 :
Restore with deionized water (or equivalent)
重组体积 :
100微升
物种活性 :
human, mouse, rat, monkey
稳定剂 :
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
储存 :
Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.
子种类 :
IgG1 kappa
同义词 :
mouse anti-AKT pS473 DyLight™ 549 conjugated Antibody, DyLight549 conjugated mouse anti-AKT pS473 Antibody, RAC-PK-alpha, Protein kinase B, PKB, C-AKT, RAC-alpha serine/threonine-protein kinase, Proto-oncogene c-Akt, AKT1, AKT 1, AKT-1
靶物种 :
人类
背景 :
AKT is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308. AKT is a cytoplasmic protein also known as AKT1, Protein Kinase B (PKB) and rac (related to A and C kinases). AKT is a key regulator of many signal transduction pathways. AKT Exhibits tight control over cell proliferation and cell viability. Overexpression or inappropriate activation of AKT is noted in many types of cancer. AKT mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. AKT has two main roles: (i) inhibition of apoptosis; (ii) promotion of proliferation.
免疫原类型 :
多肽
低内毒素 :
No
翻译后的修饰作用 :
磷酸化
样品尺寸 :
No
应用文本 :
酶联免疫吸附测定, FLISA, 流式细胞仪, IF显微镜, 免疫印迹
其他 :
User Optimized
类别 :
一抗
共轭名称 :
DyLight™ 549
2020价格 :
369.00美元
UniProt数据库 :
P31749
NCBI :
62241011
最初图像名称 :
抗微管蛋白和抗TNFa抗体-免疫印迹
更多信息或购买 :
公司信息
Rockland Immunochemicals
321 Jones Blvd
Pottstown, PA 19464
orders@rockland-inc.com
https://rockland-inc.com
800-656-7625
公司总部: 美国
Rockland Immunochemicals, Inc.位于宾夕法尼亚州的费城西部,为基础研究、应用研究及临床研究提供磷酸化位点特异性抗体及抗体相关研究工具。公司的技术平台是由一批经验丰富的科学家建立,利用In vitro及In vivo方法制备并纯化单抗及多抗,这些抗体应用于免疫印迹、ELISA、免疫组化、荧光检测、高通量筛选及诊断试剂盒生产等。常用一抗主要应用于以下研究:Akt通路、载脂蛋白、细胞凋亡/细胞周期、细胞因子、细胞信号、酶类、胞外基质、转录因子及泛素相关领域。