抗体

Zic-1 Antibody

200-401-159

100微克

多克隆

domestic rabbit

未共轭

人类, 小鼠

免疫印迹, 酶联免疫吸附测定, 免疫组化

Anti-Zic-1 (Mouse/Human) (RABBIT) Antibody - 200-401-159

Zic-1 Antibody

Anti Zic-1 Antibody is suitable for western blotting and for ELISA. Researchers should determine optimal titers for applications that are not stated below.

0.02 M钾磷酸盐, 0.15 M氯化钠, pH值7.2

多克隆

1.0毫克/毫升

by UV absorbance at 280 nm

(None)

100微克

微克

1:10, 000 - 1:50, 000

1:400

1:400

1:5,000

Expiration date is one (1) year from date of opening.

IgG

Zic1

兔

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

The whole rabbit serum used to produce this IgG fraction antibody was prepared by repeated immunizations with an 18 aa synthetic peptide from a region near the N-Terminus of mouse Zic-1. This domain is completely conserved in human ZIC-1.

干冰

Liquid (sterile filtered)

0.01%(w/v)叠氮化钠

This is an IgG preparation of whole rabbit antiserum purified by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  This antibody is directed against Zic-1 from mouse.  In general, this antibody also detects human Zic-1.  Cross-reactivity with other species is likely but has not been determined.

人类, 小鼠

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

rabbit anti-Zic-1 Antibody, Odd paired homolog Drosophila antibody, Zic 1 antibody, ZIC antibody, Zic family member 1 (odd-paired Drosophila homolog) antibody, Zic family member 1 antibody

小鼠

Anti Zic-1 Antibody recognizes the product of the Zic-1 gene, that encodes a zinc finger protein which is expressed in the developing or matured central nervous system in a highly restricted manner.  Zic-1 is expressed in granule cells that make synaptic contact with Purkinje cells.  Clearly Zic-1 is a gene critical to cerebellar pattern formation.  The expression of Zic genes is first detected at gastrulation and at neurulation, becomes restricted to the dorsal neural ectoderm and the dorsal paraxial mesoderm.  Zic-2 and Zic-3 are highly similar genes, especially in their product's zinc finger motif and by comparison of their genomic organization in that they share common exon-intron boundaries and belong to the same gene family.  By comparison in function, Zic-2 is essential for the formation of the brain and Zic-3 is important for right and left axis formation.  The Zic-1 gene has been mapped to chromosome 9 in mouse.  The 5' flanking region of the Zic-1 gene contains a region-specific enhancer determined to be essential in in vivo and in vitro deletion analysis.  The temporal profile of mRNA expression differs for each of the Zic gene products.  The Drosophila odd-paired gene is highly homologous to the Zic gene family.

多肽

No

No

酶联免疫吸附测定, 免疫组化, IF显微镜, 免疫印迹

User Optimized

一抗

未共轭

P46684

AAH60247.1

抗Zic1抗体-免疫印迹